Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,166,099	G→C	12.7%	G163A (GGC→GCC)	PP_2780 →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,166,099	0	G	C	12.7%	169.5 / 14.4	61	G163A (GGC→GCC)	PP_2780	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (25/28);  new base C (5/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGAC  >  NC_002947/3165950‑3166244                                                                                                                                                       |                                                                                                                                                  tCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCACTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATgg                                                                                                                                                   >  2:346172/1‑150 (MQ=255)       tttGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCgg                                                                                                                                             <  2:1111813/150‑1 (MQ=255)           cAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGt                                                                                                                                         >  2:1204186/1‑150 (MQ=255)              gACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGaa                                                                                                                                      <  2:564596/150‑1 (MQ=255)               aCATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAg                                                                                                                                     <  2:901812/150‑1 (MQ=255)                       ctCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTg                                                                                                                             >  2:411939/1‑150 (MQ=255)                         cAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGTCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGaa                                                                                                                                      <  2:683823/139‑1 (MQ=255)                         cAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGTCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGaa                                                                                                                                      >  1:683823/1‑139 (MQ=255)                            ccTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCaa                                                                                                                        >  1:1163779/1‑150 (MQ=255)                             ctagCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAAt                                                                                                                       <  1:346172/147‑1 (MQ=255)                              tGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATg                                                                                                                      >  2:1031983/1‑150 (MQ=255)                                 cGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTca                                                                                                                   <  2:1267314/150‑1 (MQ=255)                                    aTAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTcagca                                                                                                                <  2:134430/150‑1 (MQ=255)                                        cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                            >  2:1071598/1‑150 (MQ=255)                                        cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                            >  1:42176/1‑150 (MQ=255)                                         ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGcc                                                                                                           >  1:851150/1‑150 (MQ=255)                                         ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGcc                                                                                                           >  1:1133710/1‑150 (MQ=255)                                         ccGGCGCAGGCGCCGTCCTGGGCTCAATGGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGcc                                                                                                           >  1:1278087/1‑150 (MQ=255)                                                aGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCgg                                                                                                    <  1:1071598/150‑1 (MQ=255)                                                  gcgcCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGa                                                                                                  <  2:1163779/150‑1 (MQ=255)                                                  gcgcCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGa                                                                                                  <  1:1204186/150‑1 (MQ=255)                                                  gcgcCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGa                                                                                                  <  2:670347/150‑1 (MQ=255)                                                    gcCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGc                                                                                                <  1:1031983/150‑1 (MQ=255)                                                           tGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAg                                                                                         <  1:1259287/150‑1 (MQ=255)                                                                tCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCACCGACCGCCTTGCGCGGGTGATTGCCGATGCCATc                                                                                                                                               >  2:829402/1‑91 (MQ=255)                                                                tCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCAt                                                                                    >  2:1072985/1‑150 (MQ=255)                                                                tCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATc                                                                                                                                               <  1:829402/91‑1 (MQ=255)                                                                tCAACTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCAt                                                                                    >  2:122508/1‑150 (MQ=255)                                                                   aTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCgg                                                                                 >  2:166487/1‑150 (MQ=255)                                                                      gATGGCGCGGCAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGTAGCGCTTGCGCGGGAGCGTCCCAGGCCATCGGCaa                                                                              <  1:566693/150‑1 (MQ=255)                                                                         ggCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGc                                                                           >  1:92155/1‑150 (MQ=255)                                                                            gcgACAGGCGCAGGTCGATCCTGACAGTTCCCTGGCGT‑AGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAtt                                                                        >  1:304026/1‑150 (MQ=255)                                                                              gACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCgg                                                                                                    >  1:224581/1‑120 (MQ=255)                                                                              gACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCgg                                                                                                    <  2:224581/120‑1 (MQ=255)                                                                                                cTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCgcggcg                                                    >  1:568075/1‑150 (MQ=255)                                                                                                 tGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATcc                                                             <  1:1193617/140‑1 (MQ=255)                                                                                                 tGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATcc                                                             >  2:1193617/1‑140 (MQ=255)                                                                                                  gACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAAt                                                                             <  1:1221446/123‑1 (MQ=255)                                                                                                  gACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAAt                                                                             >  2:1221446/1‑123 (MQ=255)                                                                                                      gTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGGCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCg                                              >  2:1079542/1‑150 (MQ=255)                                                                                                       ttCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGa                                             >  2:701918/1‑150 (MQ=255)                                                                                                       ttCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGGCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGa                                             >  1:145826/1‑150 (MQ=255)                                                                                                        tCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGGCGCGTCTAGGTCGAg                                            <  2:1278087/150‑1 (MQ=255)                                                                                                         cACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGa                                                                                                                            >  1:374214/1‑69 (MQ=255)                                                                                                         cACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGa                                                                                                                            <  2:374214/69‑1 (MQ=255)                                                                                                         cACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGc                                           <  1:122508/150‑1 (MQ=255)                                                                                                           cT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGAAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTg                                         <  1:426694/150‑1 (MQ=255)                                                                                                                      gCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCg                               <  2:851150/150‑1 (MQ=255)                                                                                                                       cAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCgg                              <  2:1133710/150‑1 (MQ=255)                                                                                                                        agccaCCGCCCTGCGCGTATAATTGCCGATACCATCGTCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGCCCATTGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCggg                             <  2:568075/146‑1 (MQ=255)                                                                                                                        aaCGACCGCCTTGCGCGGGTGATTGCCGATGGCATCCGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCggg                             <  2:304026/150‑1 (MQ=255)                                                                                                                         aCGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGGCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCgggg                            <  1:1079542/150‑1 (MQ=255)                                                                                                                                  ttGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGAtt                   <  1:166487/150‑1 (MQ=255)                                                                                                                                           tGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTa          <  1:701918/150‑1 (MQ=255)                                                                                                                                            gATTGCCGATGGCATCGGCAGTGAAGGACCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAACGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGCTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAg         >  2:411302/1‑150 (MQ=255)                                                                                                                                             actGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGc        >  2:1246492/3‑150 (MQ=255)                                                                                                                                             actGCCGATGCCATCGGCAATGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                     >  2:296624/3‑89 (MQ=255)                                                                                                                                             actGCCGATGCCATCGGCAATGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                     <  1:296624/87‑1 (MQ=255)                                                                                                                                              ttGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGcc       >  1:827211/1‑150 (MQ=255)                                                                                                                                              ctGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGcc       >  1:1088904/2‑150 (MQ=255)                                                                                                                                                gCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTg     <  2:92155/150‑1 (MQ=255)                                                                                                                                                   gATGCCATCGGCAGTGAAGGCCCGGTGCGGAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGac  >  1:358906/1‑150 (MQ=255)                                                                                                                                                       gCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGa   <  1:314555/145‑1 (MQ=255)                                                                                                                                                       gCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGa   >  2:314555/1‑145 (MQ=255)                                                                                                                                                       |                                                                                                                                                  TCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACT‑GCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGAC  >  NC_002947/3165950‑3166244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑