Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,166,392 C→A 8.0% L261I (CTC→ATC) ‡ PP_2780 → 3‑oxoacyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,166,3920CA 8.0% 156.0 / 2.7 50L261I (CTC→ATC) ‡PP_27803‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base A (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

CATGACCGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGCGCCGCAAC  >  NC_002947/3166247‑3166539
                                                                                                                                                 |                                                                                                                                                   
cATGACCGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGc                                                                                                                                                 >  1:90730/1‑150 (MQ=255)
     ccGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAg                                                                                                                                            <  1:305444/150‑1 (MQ=255)
           tGTATCTGCTCGGCGCCGCCAGCAGCGAACAACGTTGGGGCACCGAACTGTGTCGGCCGATCGATCGCCATCGAAGCGGCCAAAACGGAGCGGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCaa                                                                                                                                      >  1:1254126/1‑150 (MQ=255)
            gTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAAc                                                                                                                                     <  1:411302/150‑1 (MQ=255)
            gTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAAc                                                                                                                                     >  1:319195/1‑150 (MQ=255)
               tCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGcc                                                                                                                                  >  1:623184/1‑150 (MQ=255)
               tCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGcc                                                                                                                                  >  1:192332/1‑150 (MQ=255)
                  gCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTa                                                                                                                               <  2:42176/150‑1 (MQ=255)
                  gCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTa                                                                                                                               <  1:1152169/150‑1 (MQ=255)
                    tCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGAGCGCCCgttcga                                                                                                                                         >  2:1137311/1‑133 (MQ=255)
                    tCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGAGCGCCCgttcga                                                                                                                                         <  1:1137311/138‑6 (MQ=255)
                             ccAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGc                                                                                                                    >  2:473262/1‑150 (MQ=255)
                             ccAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGc                                                                                                                    <  1:163622/150‑1 (MQ=255)
                               agcagcGAACAGCGTTGGGGCACCGGACTGTGTCAGCCGTTCGATCGCCATCGCAGTGGCTGGATTGCTGGGGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTg                                                                                                                  >  1:731575/1‑150 (MQ=255)
                               agcagcGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTg                                                                                                                  <  1:1072985/150‑1 (MQ=255)
                                  agcGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGt                                                                                                               >  1:457839/1‑150 (MQ=255)
                                   gcGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGAGCGCCCgttcgag                                                                                                                                        <  2:458492/124‑7 (MQ=255)
                                   gcGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGAGCGCCCgttcgag                                                                                                                                        >  1:458492/1‑118 (MQ=255)
                                     gAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTc                                                                                                            <  1:760710/150‑1 (MQ=255)
                                       aCAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCgg                                                                                                          <  2:1119143/150‑1 (MQ=255)
                                            gTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGgcagca                                                                                                     >  1:1236288/1‑150 (MQ=255)
                                                gggCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCt                                                                                                 <  2:1254126/150‑1 (MQ=255)
                                                     ccGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATg                                                                                            <  2:623184/150‑1 (MQ=255)
                                                      cGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTg                                                                                                                  >  1:806079/1‑127 (MQ=255)
                                                      cGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTg                                                                                                                  <  2:806079/127‑1 (MQ=255)
                                                      cGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGc                                                                                           >  2:376374/1‑150 (MQ=255)
                                                         aCTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTa                                                                                        >  1:172863/1‑150 (MQ=255)
                                                                 ggCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCa                                                                                <  2:465963/150‑1 (MQ=255)
                                                                  gCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGAt                                                                                             >  1:1065238/1‑136 (MQ=255)
                                                                  gCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGAt                                                                                             <  2:1065238/136‑1 (MQ=255)
                                                                          aTCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCgcccc                                                                       >  1:1021722/1‑149 (MQ=255)
                                                                             gCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCacccgacc                                                                    >  1:200833/1‑150 (MQ=255)
                                                                              ccATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTAc                                                                                       >  1:169983/1‑130 (MQ=255)
                                                                              ccATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTAc                                                                                       <  2:169983/130‑1 (MQ=255)
                                                                              ccATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCacccgaccc                                                                   >  2:232230/1‑150 (MQ=255)
                                                                                        ggCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCgg                                                         <  2:192332/150‑1 (MQ=255)
                                                                                         gCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCt                                                                                                 >  2:195646/1‑109 (MQ=255)
                                                                                         gCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCt                                                                                                 <  1:195646/109‑1 (MQ=255)
                                                                                           ttGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGgc                                                      >  2:241654/1‑150 (MQ=255)
                                                                                              aTTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGcgcg                                                   >  2:152811/1‑150 (MQ=255)
                                                                                                 gCGGGTGAGGGCGGTGGTTTTGTCGTGCTCTAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTg                                                <  2:262230/150‑1 (MQ=255)
                                                                                                     gTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTgg                                            <  1:241654/150‑1 (MQ=255)
                                                                                                          ggCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATg                                       >  1:1169321/1‑150 (MQ=255)
                                                                                                          ggCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATg                                       >  2:588319/1‑150 (MQ=255)
                                                                                                               tggtTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGAAGCAGCCTGGATGCGTACAAAATCACCGCACACGGCTCGCAGGGCCGGTGCGCGGTGCTGGCGATGCAggc                                  >  1:332503/1‑150 (MQ=255)
                                                                                                                 gtgtggTCGTGCTCTAAACTCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAggcgg                                <  2:1236288/145‑1 (MQ=255)
                                                                                                                             tCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTAGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATg                    >  1:350995/1‑150 (MQ=255)
                                                                                                                                    ccTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCt             <  1:588319/150‑1 (MQ=255)
                                                                                                                                    ccTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCt             <  1:376374/150‑1 (MQ=255)
                                                                                                                                     cTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTg            >  2:1122702/1‑150 (MQ=255)
                                                                                                                                               cgcTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGCGCCGCAAc  <  2:90730/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CATGACCGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGCGCCGCAAC  >  NC_002947/3166247‑3166539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑