Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 240,536 G→C 8.5% G277G (GGG→GGC)  PP_0188 → uroporphyrin‑III C‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947240,5360GC 8.5% 139.8 / 5.5 47G277G (GGG→GGC) PP_0188uroporphyrin‑III C‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base C (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

CCCGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGCAGACAAC  >  NC_002947/240387‑240680
                                                                                                                                                     |                                                                                                                                                
cccGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCggg                                                                                                                                                  <  1:1069194/150‑1 (MQ=255)
 ccGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGCTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGgc                                                                                                                                                 <  2:861435/150‑1 (MQ=255)
       tCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGCCCGGCCAGCAAc                                                                                                                                           <  2:143065/150‑1 (MQ=255)
         gACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTg                                                                                                                                         >  1:1063503/1‑150 (MQ=255)
                aCGCCTATGCGCTTGGCGCCCTGCACGCGGACGGTGCTGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  <  2:851603/150‑1 (MQ=255)
                               gcgcCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGa                                                                                                                   <  2:302508/150‑1 (MQ=255)
                                 gcCTTGACCGCCGACGGTGATGGTGCCACCAGGCTGTCGCAGTGGTGGGCAGAACTATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGc                                                                                                                 >  2:1228009/1‑150 (MQ=255)
                                   ccTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCt                                                                                                               >  2:213945/1‑150 (MQ=255)
                                   ccTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGAAAGTTAACCAGCTGCGCCTGGCCCTGAGCCt                                                                                                               <  1:554708/150‑1 (MQ=255)
                                     tGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGGGGGGGGCAGAAATATCCAAGTACTTCCAGATCGACGTCAATGACGATGCAAACGTACGTCCCTTGCTGGCCGGGACCCAAGTGAACCACCTGCGCCTCGCCCTGAGCCCGa                                                                                                             >  2:245260/1‑150 (MQ=255)
                                      gACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGcca                                                                                                                      >  2:680216/1‑139 (MQ=255)
                                      gACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGcca                                                                                                                      <  1:680216/140‑2 (MQ=255)
                                        ccgccgACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGACAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTa                                                                                                          >  2:380586/1‑150 (MQ=255)
                                               cGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCa                                                                                                   <  1:213945/150‑1 (MQ=255)
                                                 gTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAgg                                                                                                 >  2:1100957/1‑150 (MQ=255)
                                                            agcagGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGggcg                                                                                      >  2:855536/1‑150 (MQ=255)
                                                            agcagGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGggcg                                                                                      >  1:954/1‑150 (MQ=255)
                                                                    gTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCaa                                                                              <  1:1100957/150‑1 (MQ=255)
                                                                      cGCAGTGGTGGACAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATg                                                                            <  1:380586/150‑1 (MQ=255)
                                                                       gCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  <  1:626689/95‑1 (MQ=255)
                                                                       gCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  >  2:626689/1‑95 (MQ=255)
                                                                        cAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGt                                                                          <  2:1063503/150‑1 (MQ=255)
                                                                                 gCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAg                                                                 <  2:682989/150‑1 (MQ=255)
                                                                                   aGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGt                                                               >  2:277397/1‑150 (MQ=255)
                                                                                      aaTATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGAGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTa                                                            <  2:1142909/150‑1 (MQ=255)
                                                                                             aaGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  <  2:1235759/73‑1 (MQ=255)
                                                                                             aaGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  >  1:1235759/1‑73 (MQ=255)
                                                                                                   ttCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTg                                               >  2:23235/1‑150 (MQ=255)
                                                                                                   ttCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTg                                               <  1:277397/150‑1 (MQ=255)
                                                                                                     ccAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCAAAGGTGTACACGCAGGCGCTGga                                             >  1:390450/1‑150 (MQ=255)
                                                                                                      cAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGgac                                            <  2:390450/150‑1 (MQ=255)
                                                                                                        gATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGgacga                                          <  1:411557/150‑1 (MQ=255)
                                                                                                        gATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGgacga                                          >  2:1239755/1‑150 (MQ=255)
                                                                                                         aTCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGCGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCGCAAGGGTGATGCCAAGGTTTACACGCAGGCGCTGgacgac                                         <  1:1228009/150‑1 (MQ=255)
                                                                                                               ttCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGAGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGc                                   >  1:1040786/1‑150 (MQ=255)
                                                                                                                 cAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAg                                 <  2:708782/150‑1 (MQ=255)
                                                                                                                        gATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCGGGGTCAGTATGCGGCGCTCAATGGTGATGCAACGGTTTACACACAGACGCTGGACTACGCCCGCAGCGTActg                          >  1:542579/1‑150 (MQ=255)
                                                                                                                          tGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATTGGTCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTActgct                        >  1:730706/1‑150 (MQ=255)
                                                                                                                              aaCGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGcc                    >  1:741041/1‑150 (MQ=255)
                                                                                                                                 gTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAAc                 <  2:954/150‑1 (MQ=255)
                                                                                                                                  tGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACt                >  2:170582/1‑150 (MQ=255)
                                                                                                                                     ttcagTTGCTGCCCGGCCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCa             >  2:1058578/5‑150 (MQ=255)
                                                                                                                                        agTTGCTGCCCGGCCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATg          >  1:26610/2‑150 (MQ=255)
                                                                                                                                            gCTGCCCGGCCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGCAGa      <  1:1058578/150‑1 (MQ=255)
                                                                                                                                                gCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGccc                                     >  2:616601/1‑115 (MQ=255)
                                                                                                                                                gCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGccc                                     <  1:616601/115‑1 (MQ=255)
                                                                                                                                                gCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCATGCCTCAAGGTCAGGTCCAGTGGGCGGCGCTCCATGGTTATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGCAGACAAc  <  1:926699/150‑1 (MQ=255)
                                                                                                                                                  cGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGggcgg                                                                                     <  2:12480/65‑1 (MQ=255)
                                                                                                                                                  cGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGggcgg                                                                                     >  1:12480/1‑65 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CCCGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGCAGACAAC  >  NC_002947/240387‑240680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑