Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,385,130 A→G 7.4% L110L (CTT→CTC)  PP_2986 ← oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,385,1300AG 7.4% 155.1 / 2.9 54L110L (CTT→CTC) PP_2986oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (29/21);  new base G (2/2);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

AGCGAGGATCTTGCTGCGGTAGTAGTCATCGGCGTCGGCGTCGGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATG  >  NC_002947/3384983‑3385277
                                                                                                                                                   |                                                                                                                                                   
aGCGAGGATCTTGCTGCGGTAGTAGTCATCGGCGTCGGCGTCGGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTTGGCCTGCCCAAg                                                                                                                                                   <  2:1013408/150‑1 (MQ=255)
   gAGGATCTTGCTGCGGTAGTAGTCATCGGCGTCGGCGTCGGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAg                                                                                                                                                <  1:862220/150‑1 (MQ=255)
           tgctgcCGTAGTAGTCATCGGCGTCGGCGTCCGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGACCCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCcgcg                                                                                                                                        <  1:1040704/150‑1 (MQ=255)
             ctgcGGTAGTAGTCATCGGCGTCGGCGTCGGCCCTCAGGTAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGgcgtc                                                                                                                                           <  1:44966/145‑3 (MQ=255)
             ctgcGGTAGTAGTCATCGGCGTCGGCGTCGGCCCTCAGGTAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGcgcc                                                                                                                                           >  2:44966/1‑145 (MQ=255)
              tgcGGTAGTAGTCATCGGCGTCGGCGTCGGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCaa                                                                                                                                                    <  2:82362/135‑1 (MQ=255)
              tgcGGTAGTAGTCATCGGCGTCGGCGTCGGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCaa                                                                                                                                                    >  1:82362/1‑135 (MQ=255)
                     gtagtCATCGGCGTCGGCGTCGGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGacac                                                                                                                              <  2:186917/150‑1 (MQ=255)
                           aTCGGCGTCGGCGTCGGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTAt                                                                                                                        <  2:528709/150‑1 (MQ=255)
                               gcgtcggcgtcgGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTAGTATGCCGGCTCGCTAGGCATGTCCAAGCAGGGGCCGCGTACCCGACACGTTTATCTGt                                                                                                                    >  2:345599/1‑150 (MQ=255)
                                 gtcggcgtcggcCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTc                                                                                                                  <  2:433535/150‑1 (MQ=255)
                                             ccTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTTGAGCAGgcct                                                                                                                                            >  2:965736/1‑110 (MQ=255)
                                             ccTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTTGAGCAGgcct                                                                                                                                            >  2:325235/1‑110 (MQ=255)
                                             ccTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTTGAGCAGgcct                                                                                                                                            <  1:965736/112‑3 (MQ=255)
                                             ccTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTTGAGCAGgcct                                                                                                                                            <  1:325235/112‑3 (MQ=255)
                                              cTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAg                                                                                                     <  1:345599/150‑1 (MQ=255)
                                               tCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCCATGCGTGAGGTAGGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGt                                                                                                    >  2:437601/1‑150 (MQ=255)
                                                   gCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGa                                                                                                                >  2:834839/1‑134 (MQ=255)
                                                   gCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGa                                                                                                                <  1:834839/134‑1 (MQ=255)
                                                   gCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCt                                                                                                >  2:29693/1‑150 (MQ=255)
                                                   gCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCt                                                                                                >  2:753806/1‑150 (MQ=255)
                                                     aGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTgc                                                                                              >  1:346150/1‑150 (MQ=255)
                                                          tcctcGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGccc                                                                                         >  1:1144723/1‑150 (MQ=255)
                                                           cctcGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCt                                                                                        >  1:1024163/1‑150 (MQ=255)
                                                           cctcGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGACCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCAATTGGCTTCCGCCCt                                                                                        >  2:714349/1‑150 (MQ=255)
                                                            ctcGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCtt                                                                                       >  1:1076426/1‑150 (MQ=255)
                                                             tcGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTg                                                                                      >  2:1098208/1‑150 (MQ=255)
                                                               gATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTa                                                                                    <  2:1144723/150‑1 (MQ=255)
                                                               gATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTa                                                                                    <  2:1076426/150‑1 (MQ=255)
                                                                       tGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATcgc                                                                            <  1:305489/150‑1 (MQ=255)
                                                                       tGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATcgc                                                                            >  2:644119/1‑150 (MQ=255)
                                                                        gCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATcgcg                                                                           >  2:390536/1‑150 (MQ=255)
                                                                                         gCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGa                                                                                                                >  1:4201/1‑96 (MQ=255)
                                                                                         gCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGa                                                                                                                <  2:4201/96‑1 (MQ=255)
                                                                                                aaTGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAacac                                                   >  1:570486/1‑150 (MQ=255)
                                                                                                 aTGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACAcc                                                  <  1:29693/150‑1 (MQ=255)
                                                                                                    gAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTa                                               <  1:753806/150‑1 (MQ=255)
                                                                                                    gAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTa                                               >  2:690574/1‑150 (MQ=255)
                                                                                                                      cgtcgtATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGAt                             >  1:1147032/1‑150 (MQ=255)
                                                                                                                       gtcgtATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGAtg                            >  1:857296/1‑150 (MQ=255)
                                                                                                                        tcgtATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGAtgc                           >  2:514403/1‑150 (MQ=255)
                                                                                                                                cGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGt                                                                                                                    >  1:32131/1‑53 (MQ=255)
                                                                                                                                cGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGt                                                                                                                    <  2:32131/53‑1 (MQ=255)
                                                                                                                                 ggCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTAt                                              >  2:1071941/1‑122 (MQ=255)
                                                                                                                                 ggCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTAt                                              <  1:1071941/122‑1 (MQ=255)
                                                                                                                                 ggCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGc                  >  1:194648/1‑150 (MQ=255)
                                                                                                                                   ccccGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGt                <  2:194648/150‑1 (MQ=255)
                                                                                                                                   ccccGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGt                <  1:714349/150‑1 (MQ=255)
                                                                                                                                   ccccGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGt                >  2:854166/1‑150 (MQ=255)
                                                                                                                                      cGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGa             <  1:1098208/150‑1 (MQ=255)
                                                                                                                                       gTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGAc            >  1:229950/1‑150 (MQ=255)
                                                                                                                                         aGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCt          >  1:1237645/1‑150 (MQ=255)
                                                                                                                                             cTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGAAGCTGTGCAAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGc      >  2:675998/1‑150 (MQ=255)
                                                                                                                                                 tCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTc               <  1:1257640/137‑1 (MQ=255)
                                                                                                                                                 tCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTc               >  2:1257640/1‑137 (MQ=255)
                                                                                                                                                 tCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATg  >  2:40921/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGCGAGGATCTTGCTGCGGTAGTAGTCATCGGCGTCGGCGTCGGCCCTCAGGCAGTTCTCCTCGATGGACGTGCCAGGTGCGCCGTTGAGCACAGCAATGGAAGACGTATGGATGAACCGTCGTATGCCGGCCCCGTAGGCCTGCTCAAGCAGGCGCCGCGTACCCGACACGTTTATCTGTTCGAGTTCTTTCCAGTGGCTGCCGCCCTTGTAGTTATCGCGAAAGAACGACGCTGTGTGAAACACCGTATCGCAACCTTGCAGCGATGCTGCGAATGCGTCGACCTCGGCCATG  >  NC_002947/3384983‑3385277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑