Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,463,079 G→C 8.3% A441G (GCC→GGC)  PP_3078 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,463,0790GC 8.3% 145.7 / 4.4 48A441G (GCC→GGC) PP_3078ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (21/23);  new base C (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

GATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAACTCGCCCGCATCCGGCAACTGGCGAGCGGCCA  >  NC_002947/3462931‑3463223
                                                                                                                                                    |                                                                                                                                                
gATCTGGGGCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGcagggccagggc                                                                                                                                                 >  2:373962/1‑150 (MQ=255)
      ggCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCaa                                                                                                                                           <  2:1202137/150‑1 (MQ=255)
      ggCCAGGTTGCGCGTCCACGGGAGCAGCAGGGGTTCAAGGCCCGCCTGGCCATTGAGGAACGGGAGCGCCAGCGGCGGGCCCTGGCGATCGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCaa                                                                                                                                           <  2:620111/150‑1 (MQ=255)
       gCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAAc                                                                                                                                          >  1:545278/1‑150 (MQ=255)
         cAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAActg                                                                                                                                        <  1:431087/150‑1 (MQ=255)
            gTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAActgc                                                                                                                                       <  2:795377/148‑1 (MQ=255)
            gTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAActgc                                                                                                                                       >  1:795377/1‑148 (MQ=255)
               gcgcTTCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTg                                                                                                                                  >  2:1286854/1‑150 (MQ=255)
                    tCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGga                                                                                                                             <  1:373962/150‑1 (MQ=255)
                     ccACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGgat                                                                                                                            <  2:1192954/150‑1 (MQ=255)
                     ccACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGCCCTGGCCCTGct                                                                                                                                            >  2:701478/1‑133 (MQ=255)
                     ccACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGCCCTGGCCCTGct                                                                                                                                            <  1:701478/134‑2 (MQ=255)
                       aCGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGgatga                                                                                                                          >  2:64563/1‑150 (MQ=255)
                          gcagcagcaGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCTTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATcc                                                                                                                       <  1:52346/150‑1 (MQ=255)
                            agcagcagGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATcccc                                                                                                                     <  2:161789/150‑1 (MQ=255)
                              cagcagGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGa                                                                                                                   >  2:14400/1‑150 (MQ=255)
                              cagcagGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGa                                                                                                                   <  1:64563/150‑1 (MQ=255)
                                  agGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCa                                                                                                               >  2:27902/1‑150 (MQ=255)
                                   ggCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCAt                                                                                                              >  1:1229073/1‑150 (MQ=255)
                                            ggCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCg                                                                                                     <  1:1016369/150‑1 (MQ=255)
                                             gCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGCCCTGGCCCTGctc                                                                                                                                           <  2:410298/111‑3 (MQ=255)
                                             gCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGCCCTGGCCCTGctc                                                                                                                                           >  1:410298/1‑109 (MQ=255)
                                                  ccTCGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAg                                                                                               <  1:1286854/150‑1 (MQ=255)
                                                        cATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCg                                                                                                            <  1:113300/131‑1 (MQ=255)
                                                        cATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCg                                                                                                            >  2:113300/1‑131 (MQ=255)
                                                             aGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCgtg                                                                                    <  2:1229073/150‑1 (MQ=255)
                                                                   gTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAac                                                                              <  1:62370/150‑1 (MQ=255)
                                                                         gCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCa                                                                        <  1:27902/150‑1 (MQ=255)
                                                                           cAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATc                                                                      >  1:2249/1‑150 (MQ=255)
                                                                            aGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCt                                                                     <  1:1127604/150‑1 (MQ=255)
                                                                                  gTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACt                                                               >  2:356190/1‑150 (MQ=255)
                                                                                       cTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCAt                                                                                                              <  1:9995/98‑1 (MQ=255)
                                                                                       cTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCAt                                                                                                              >  2:9995/1‑98 (MQ=255)
                                                                                       cTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGcc                                                          >  2:911910/1‑150 (MQ=255)
                                                                                        tGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGcagggccagggc                                                                                                                                                 >  2:434540/1‑62 (MQ=255)
                                                                                        tGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGcagggccagggc                                                                                                                                                 <  1:434540/62‑1 (MQ=255)
                                                                                              ttGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAg                                                   <  1:533410/150‑1 (MQ=255)
                                                                                                 aCCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCACAAgggg                                                >  2:22382/1‑150 (MQ=255)
                                                                                                  ccAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAggggg                                               >  1:1194419/1‑150 (MQ=255)
                                                                                                   cAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGc                                              <  1:911910/150‑1 (MQ=255)
                                                                                                   cAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGc                                              <  1:1063894/150‑1 (MQ=255)
                                                                                                              cTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCa                                   >  1:527822/1‑150 (MQ=255)
                                                                                                               ttCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCcgc                                                        <  1:408981/128‑1 (MQ=255)
                                                                                                               ttCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCcgc                                                        >  2:408981/1‑128 (MQ=255)
                                                                                                                       gCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAaca                                                                                         <  1:1164390/87‑1 (MQ=255)
                                                                                                                       gCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAaca                                                                                         >  2:1164390/1‑87 (MQ=255)
                                                                                                                        ccAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAAc                                 >  1:317264/1‑142 (MQ=255)
                                                                                                                        ccAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAAc                                 <  2:317264/142‑1 (MQ=255)
                                                                                                                                  tGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAACTCGCCCGCATCCGGCAAc               >  2:1229672/1‑150 (MQ=255)
                                                                                                                                   gTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAACTCGCCCGCATCCGGCAACt              >  1:365655/1‑150 (MQ=255)
                                                                                                                                    ttCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAACTCGCCCGCATCCGGCAACTg             >  1:946640/1‑150 (MQ=255)
                                                                                                                                               ccagggccTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAACTCGACCGCATCCGGCAACTGGCGAGCGGCCa  >  1:961/1‑150 (MQ=255)
                                                                                                                                               ccagggccTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAATGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAACTCGcc                            <  2:1017232/124‑1 (MQ=255)
                                                                                                                                               ccagggccTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAATGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAACTCGcc                            >  1:1017232/1‑124 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GATCTGGGCCAGGTTGCGCTTCCACGGCAGCAGCAGGCGTTCAAGGCCCGCCTGGCCATTGAGGAACGTGAACGCCAGCGGCGTGCCCTGGCTATTGACCAGGCGGTCGCCTTCGGGGTGCCAGCCGGCTTGTTCGAGCAGGGCCAGGGCCTGCAACTGCTGTTGACGGATGATCCCCGAGCCATCGGTGACCGGGGCAGTGAACACCGTGGTGAACACTTCATCTGGCACTTGGCCGCGCAAGGGGGCGAGCAGCTTCAACTCGCCCGCATCCGGCAACTGGCGAGCGGCCA  >  NC_002947/3462931‑3463223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑