Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,528,609 T→A 6.7% L128Q (CTG→CAG)  dnaEB → error‑prone DNA polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,528,6090TA 6.7% 180.8 / 4.6 60L128Q (CTG→CAG) dnaEBerror‑prone DNA polymerase
Reads supporting (aligned to +/- strand):  ref base T (31/25);  new base A (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

GACGGGCCCAAGCTGGTGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTGCAACGGTTGCGGGCACTGGCGG  >  NC_002947/3528467‑3528758
                                                                                                                                               |                                                                                                                                                     
gACGGGCCCAAGCTGGGGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGTGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGcc                                                                                                                                                <  2:541169/150‑1 (MQ=255)
     gCCCAAGCTGGTGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTtgtg                                                                                                                                           <  1:712689/150‑1 (MQ=255)
      cccAAGCTGGTGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTgtgg                                                                                                                                          <  2:1189545/150‑1 (MQ=255)
      cccAAGCTGGTGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCAGCAGGCCTtggtg                                                                                                                                          <  2:51539/150‑4 (MQ=255)
       ccAAGCTGGTGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CCACAAGGCCAGCAGGCCTtggtgg                                                                                                                                         >  1:964898/1‑146 (MQ=255)
        cAAGCTGGTGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCCGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTgtgggt                                                                                                                                        >  2:63161/1‑150 (MQ=255)
        cAAGCTGGTGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTgtgggt                                                                                                                                        >  2:549753/1‑150 (MQ=255)
        cAAGCTGGTGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTgtgggt                                                                                                                                        <  2:1106183/150‑1 (MQ=255)
                  ctgctgGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CA‑CAAGGCCAGCAGGCCTtggtggt                                                                                                                                        <  1:599077/139‑6 (MQ=255)
                  ctgctgGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CA‑CAAGGCCAGCAGGCCTtggtggt                                                                                                                                        >  2:599077/1‑134 (MQ=255)
                      tgGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCctg                                                                                                                                                      >  1:1001780/1‑122 (MQ=255)
                      tgGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCctg                                                                                                                                                      <  2:1001780/122‑1 (MQ=255)
                       ggTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTtgt                                                                                                                                            >  1:1160336/1‑131 (MQ=255)
                       ggTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTtgt                                                                                                                                            <  2:1160336/131‑1 (MQ=255)
                           gAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGAt                                                                                                                     >  1:206986/1‑150 (MQ=255)
                                 cTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCt                                                                                                               >  2:271690/1‑150 (MQ=255)
                                            ccAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCct                                                                                                                                                       <  2:1084041/99‑1 (MQ=255)
                                            ccAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCct                                                                                                                                                       >  1:1084041/1‑99 (MQ=255)
                                               gAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCt                                                                                                 >  2:716836/1‑150 (MQ=255)
                                                 aCCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTACAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGCCGCGTGGCTgc                                                                                               >  2:154078/1‑150 (MQ=255)
                                                      tgCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAgt                                                                                          <  1:271690/150‑1 (MQ=255)
                                                          cgcTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGGCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAgtgtgt                                                                                      >  1:235554/1‑150 (MQ=255)
                                                           gcTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACAGGCGCGTGAATGCGCAGGGTGtt                                                                                     >  2:913609/1‑150 (MQ=255)
                                                               aTCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCt                                                                                 <  1:913609/150‑1 (MQ=255)
                                                                tCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTg                                                                                >  2:149981/1‑150 (MQ=255)
                                                                 cACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACAAGCTGTGTCGTGATGACTTGCTGCTGCAC‑AACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGa                                                                               <  2:465758/150‑1 (MQ=255)
                                                                  aCCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAg                                                                              <  2:206986/150‑1 (MQ=255)
                                                                        gCCCGCCGCCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCtg                                                                        >  1:1151957/1‑150 (MQ=255)
                                                                          ccGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTtgt                                                                                                                                            <  2:857203/80‑1 (MQ=255)
                                                                          ccGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTtgt                                                                                                                                            >  1:857203/1‑80 (MQ=255)
                                                                                gcggGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGtggctggc                                                                <  1:716836/150‑1 (MQ=255)
                                                                                  gggCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTtgt                                                                                                                                            <  1:1167245/72‑1 (MQ=255)
                                                                                  gggCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTtgt                                                                                                                                            >  2:1167245/1‑72 (MQ=255)
                                                                                     cAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTgg                                                                                                   <  1:610372/110‑1 (MQ=255)
                                                                                     cAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTgg                                                                                                   >  2:610372/1‑110 (MQ=255)
                                                                                               ggCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCg                                                 >  1:317064/1‑150 (MQ=255)
                                                                                                gCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCgg                                                <  2:1151957/150‑1 (MQ=255)
                                                                                                  gTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGgcgc                                                                                                       >  1:186929/1‑93 (MQ=255)
                                                                                                  gTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGgcgc                                                                                                       <  2:186929/93‑1 (MQ=255)
                                                                                                     ccAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGcc                                                               >  2:1226241/1‑130 (MQ=255)
                                                                                                     ccAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGcc                                                               <  1:1226241/130‑1 (MQ=255)
                                                                                                     ccAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAg                                           <  1:3904/150‑1 (MQ=255)
                                                                                                      cAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAgt                                                                                          >  2:1210923/1‑102 (MQ=255)
                                                                                                      cAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAgt                                                                                          <  1:1210923/102‑1 (MQ=255)
                                                                                                      cAGCTGTTTCGTGATGACTTGCTACT‑CACAGACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGt                                          >  1:674835/1‑150 (MQ=255)
                                                                                                         cTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGAc                                       <  2:759358/150‑1 (MQ=255)
                                                                                                         cTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGAc                                       >  1:759358/1‑150 (MQ=255)
                                                                                                                gTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCg                                <  1:63161/150‑1 (MQ=255)
                                                                                                                   atgaCTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTgc                             >  1:58712/1‑150 (MQ=255)
                                                                                                                     gaCTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGa                                                                               <  2:1034027/98‑1 (MQ=255)
                                                                                                                     gaCTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGa                                                                               >  1:1034027/1‑98 (MQ=255)
                                                                                                                     gaCTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGtggctgg                                                                 >  2:454478/1‑112 (MQ=255)
                                                                                                                     gaCTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGtggctgg                                                                 <  1:454478/112‑1 (MQ=255)
                                                                                                                        ttGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTg                        >  2:81953/1‑150 (MQ=255)
                                                                                                                        ttGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTg                        >  1:387095/1‑150 (MQ=255)
                                                                                                                             gctgcAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTACTAACGGCGCGTGGCTGCACAGGTTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTGCAACg                   >  1:155821/1‑150 (MQ=255)
                                                                                                                              ctgcAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTGCAACgg                  >  1:21569/1‑150 (MQ=255)
                                                                                                                               tgcAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTGCAACGGt                 >  1:127567/1‑150 (MQ=255)
                                                                                                                                  ac‑caccAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGtgg                                                                     >  2:377349/1‑95 (MQ=255)
                                                                                                                                  ac‑caccAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGtgg                                                                     <  1:377349/95‑1 (MQ=255)
                                                                                                                                     caccaAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCCGCAACGGTTGCgg            <  1:628634/150‑1 (MQ=255)
                                                                                                                                       ccaAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTg                                                            >  2:415502/1‑100 (MQ=255)
                                                                                                                                       ccaAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTg                                                            <  1:415502/100‑1 (MQ=255)
                                                                                                                                        caAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTCAGCCAGCGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTGCAACGGTTGCGGGCa         >  2:364644/1‑150 (MQ=255)
                                                                                                                                               tgctgGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTGCAACGGTTGCGGGCACTggcgg  >  1:572225/1‑150 (MQ=255)
                                                                                                                                               tgctgGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTGCAACGGTTGCGGGCACTggcgg  <  1:1143893/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GACGGGCCCAAGCTGGTGCTGCTGGTCGAAAACCTGACGGGTTACCAGAACCTGTGCGCGCTGATCACCCGTGCCCGGCGGCGGGCAGAGAAGGGGGCGTACCAGCTGTTTCGTGATGACTTGCTGCTGCAC‑CACCAAGGCCTGCTGGCCTTGTGGGTGGCCGCTGACAGTGGCGATACTGCTACCGGCGCGTGGCTGCGCAGTGTGTTCGCTGAGCGCCTGTGGCTGGCCGTGCACCTGCATCGGGGCAGTGACGATGCCGTGCGCCTGCAACGGTTGCGGGCACTGGCGG  >  NC_002947/3528467‑3528758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑