Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,641,930 C→A 8.0% G440C (GGC→TGC)  PP_3209 ← isoxanthopterin deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,641,9300CA 8.0% 151.5 / 2.4 50G440C (GGC→TGC) PP_3209isoxanthopterin deaminase
Reads supporting (aligned to +/- strand):  ref base C (23/23);  new base A (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCATGCCGACCTGCAGCGTGCCGATGGCGTC  >  NC_002947/3641785‑3642077
                                                                                                                                                 |                                                                                                                                                   
gcgcAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCt                                                                                                                                                 >  2:986372/1‑150 (MQ=255)
gcgcAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCt                                                                                                                                                 >  1:8487/1‑150 (MQ=255)
      ggCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGc                                                                                                                                           <  1:1276768/150‑1 (MQ=255)
       gCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCa                                                                                                                                          >  1:868427/1‑150 (MQ=255)
               cTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGc                                                                                                                                  >  2:666269/1‑150 (MQ=255)
                            cAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAAt                                                                                                                                        <  1:850847/131‑1 (MQ=255)
                            cAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAAt                                                                                                                                        >  2:850847/1‑131 (MQ=255)
                                tCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCAGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCAcc                                                                                                                 >  2:669214/1‑150 (MQ=255)
                                   tGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGcc                                                                                                              >  2:1135880/1‑150 (MQ=255)
                                      gcgTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAAc                                                                                                           <  1:75886/150‑1 (MQ=255)
                                      gcgTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCAGCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAAc                                                                                                           <  1:838787/150‑1 (MQ=255)
                                           cccccGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCAGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTg                                                                                                      <  1:669214/150‑1 (MQ=255)
                                              ccGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTgggg                                                                                                   <  2:592840/150‑1 (MQ=255)
                                              ccGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTgggg                                                                                                   <  1:793673/150‑1 (MQ=255)
                                              ccGCGGCCTGTTGCAGGCGAAATACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTgggg                                                                                                   >  1:837847/1‑150 (MQ=255)
                                                  ggCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGAt                                                                                               <  1:895898/150‑1 (MQ=255)
                                                    ccTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGc                                                                                             >  1:572292/1‑150 (MQ=255)
                                                     cTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGATGTCCAAGCCAGGAATCGTGTTGGTTTATAAGTTCAGCCGTCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGcc                                                                                            >  1:91179/1‑150 (MQ=255)
                                                      tGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGccc                                                                                           >  2:657971/1‑150 (MQ=255)
                                                       gTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCATCGTCTGACGGCCACCGCCAACCACTGGGGCGATGCCCa                                                                                          <  1:666269/150‑1 (MQ=255)
                                                           cAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATc                                                                                      <  1:1135880/150‑1 (MQ=255)
                                                            aGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCa                                                                                     >  2:613235/1‑150 (MQ=255)
                                                             ggCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCAt                                                                                    >  1:258932/1‑150 (MQ=255)
                                                                         ccTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGt                                                                        >  1:594270/1‑150 (MQ=255)
                                                                             gCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCGCTTCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCACAGATAATGCAGACCGAAGTAGCg                                                                    >  1:403968/1‑150 (MQ=255)
                                                                              cGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCgg                                                                   <  2:572292/150‑1 (MQ=255)
                                                                              cGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCgg                                                                   <  1:1204098/150‑1 (MQ=255)
                                                                               gTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCCATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCggg                                                                  <  1:1121618/150‑1 (MQ=255)
                                                                                     ggCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGAt                                                            <  1:92361/150‑1 (MQ=255)
                                                                                          cccAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAgg                                                       >  1:763465/1‑150 (MQ=255)
                                                                                           ccAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGc                                                      >  1:881089/1‑150 (MQ=255)
                                                                                             aGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGa                                                    <  1:305676/150‑1 (MQ=255)
                                                                                                     gAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGc                                            <  1:986372/150‑1 (MQ=255)
                                                                                                        gTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAg                                         <  2:815483/150‑1 (MQ=255)
                                                                                                           cAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACTGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTc                                      >  1:1031860/1‑150 (MQ=255)
                                                                                                            aaGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTcc                                     >  2:509114/1‑150 (MQ=255)
                                                                                                               ccAGGAAGCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGAAATGCACGCAGCGTCGGACGGCCCACGCCAACCACTGGGGCGAAGCCCGGATCATGCGGACCGAAGTAGCGGGGTGTATGAAGTCGAGAGATGGGCAGGgccgcc                                  >  1:826523/1‑150 (MQ=255)
                                                                                                                 aGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCcgccgc                                <  1:509114/150‑1 (MQ=255)
                                                                                                                         cATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCgg                                                                   <  2:119570/107‑1 (MQ=255)
                                                                                                                         cATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCgg                                                                   >  1:119570/1‑107 (MQ=255)
                                                                                                                         cATCGTTTTCCACGCTCAGGCGGCAGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCATg                            <  2:624549/146‑1 (MQ=255)
                                                                                                                         cATCGTTTTCCACGCTCAGGCGGCAGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCATg                            >  1:624549/1‑146 (MQ=255)
                                                                                                                           tCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCATGCCGAcc                      >  2:639740/1‑150 (MQ=255)
                                                                                                                              ttttCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCC‑CCGCCAACCACTGGGGCGATGccc                                                                                           <  1:653708/77‑1 (MQ=255)
                                                                                                                              ttttCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCC‑CCGCCAACCACTGGGGCGATGccc                                                                                           >  2:653708/1‑77 (MQ=255)
                                                                                                                               tttCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCATGCCGACCTGCa                  <  2:403968/150‑1 (MQ=255)
                                                                                                                                   cACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCATGCCGACCTGCAGCGt              <  2:258932/150‑1 (MQ=255)
                                                                                                                                     cGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCATGCCGACCTGCAGCGTGc            <  1:639740/150‑1 (MQ=255)
                                                                                                                                      gCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATg                                                                                   <  1:25953/78‑1 (MQ=255)
                                                                                                                                      gCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATg                                                                                   >  2:25953/1‑78 (MQ=255)
                                                                                                                                               gccgccTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCATGCCGACCTGCAGCGTGCCGATGGCGTc  >  2:1130635/1‑150 (MQ=255)
                                                                                                                                                 cgccTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCAt                             <  2:674210/121‑1 (MQ=255)
                                                                                                                                                 cgccTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCAt                             >  1:674210/1‑121 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCGCAGGGCCCGCACCTGCAGAGGCGTCCAGCTCATGCGCGTACCCCCGCGGCCTGTTGCAGGCGAAGTACAGCCTGGCGTGCCTGGCTGCCCAGTTCGGCGAGGTCCAAGCCAGGAATCGCATCGTTTTCCACGCTCAGGCGGCCGCCTACCAGCAATGCCCGCAGCGTCGGACGGCCACCGCCAACCACTGGGGCGATGCCCAGATCATGCAGACCGAAGTAGCGGGGTTGATCAAGGCGATAGATCGCCAGGTCCGCCGCCATGCCGACCTGCAGCGTGCCGATGGCGTC  >  NC_002947/3641785‑3642077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑