Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,762,208 T→C 6.9% L505L (TTG→CTG)  PP_3325 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,762,2080TC 6.9% 167.5 / 2.4 58L505L (TTG→CTG) PP_3325ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (28/26);  new base C (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CAGGAAAAGGACGTTGCCACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCGAGAGCGTGGCGGCGGGCGAAGCG  >  NC_002947/3762067‑3762342
                                                                                                                                             |                                                                                                                                      
cAGGAAAAGGACGTTGCCACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTAc                                                                                                                                <  2:64499/150‑1 (MQ=255)
 aGGAAAAGGACGTTGCCACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACg                                                                                                                               >  2:964565/1‑150 (MQ=255)
      aaGGACGTTGCCACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGAcc                                                                                                                          <  2:508313/150‑1 (MQ=255)
            gTTGCCACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCCTgg                                                                                                                                     >  1:850187/1‑133 (MQ=255)
            gTTGCCACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCCTgg                                                                                                                                     <  2:850187/133‑1 (MQ=255)
            gTTGCCACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCCTgg                                                                                                                                     <  2:208424/133‑1 (MQ=255)
            gTTGCCACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCCTgg                                                                                                                                     >  1:208424/1‑133 (MQ=255)
                ccACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGc                                                                                                                >  1:341692/1‑150 (MQ=255)
                 cacaCCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCt                                                                                                               >  1:51507/1‑150 (MQ=255)
                  acacCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTc                                                                                                                    >  2:823744/1‑144 (MQ=255)
                  acacCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTc                                                                                                                    <  1:823744/144‑1 (MQ=255)
                     ccGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCCTCCACTTCGTTCACACCCAAAACCCTCGGAACCCCGGGCCAGGTGTACATGCCGGAGAATGGCTTGGGTTACGAGACCGGAGTCAAGCTCGAc                                                                                                           <  2:787686/150‑1 (MQ=255)
                     ccGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGAc                                                                                                           >  2:362989/1‑150 (MQ=255)
                          cATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTtct                                                                                                      <  2:837519/150‑1 (MQ=255)
                           aTCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCtt                                                                                                                                       >  1:133064/1‑116 (MQ=255)
                           aTCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCtt                                                                                                                                       <  2:133064/116‑1 (MQ=255)
                                ggTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTtctc                                                                                                     >  1:1195594/1‑145 (MQ=255)
                                ggTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTtctc                                                                                                     <  2:1195594/145‑1 (MQ=255)
                                      gtACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCt                                                                                          >  1:52789/1‑150 (MQ=255)
                                       tACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTg                                                                                         >  1:446005/1‑150 (MQ=255)
                                                     gCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTg                                                                                                                                      <  2:1003636/91‑1 (MQ=255)
                                                     gCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTg                                                                                                                                      >  1:1003636/1‑91 (MQ=255)
                                                        aGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCt                                                                        <  1:37164/150‑1 (MQ=255)
                                                                 gtTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCatat                                                               <  1:182657/150‑1 (MQ=255)
                                                                  ttCGCCAGCGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTTAAAAGGGCTTGGGTTACGAGCCCGGACTCAAGCTCGACCTTCTCGACAGCCGCCTGGGTGCGAACATCGCGCTGTTCCATATc                                                              <  2:636901/150‑1 (MQ=255)
                                                                  ttCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATc                                                              <  1:362989/150‑1 (MQ=255)
                                                                     gCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGa                                                                                                                       >  1:211219/1‑90 (MQ=255)
                                                                     gCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGa                                                                                                                       <  2:211219/90‑1 (MQ=255)
                                                                       cAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACaa                                                         <  2:446005/150‑1 (MQ=255)
                                                                         aCGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAgg                                                       >  1:258629/1‑150 (MQ=255)
                                                                              tCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAAc                                                  >  1:210124/1‑150 (MQ=255)
                                                                                      gTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGtcatca                                           <  2:749501/149‑1 (MQ=255)
                                                                                      gTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGtcatca                                           >  1:749501/1‑149 (MQ=255)
                                                                                       ttCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATcacc                                         >  1:693003/1‑150 (MQ=255)
                                                                                                aaCACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCaa                                                                                                                  <  2:841037/68‑1 (MQ=255)
                                                                                                aaCACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCaa                                                                                                                  <  2:840466/68‑1 (MQ=255)
                                                                                                aaCACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCaa                                                                                                                  >  1:841037/1‑68 (MQ=255)
                                                                                                aaCACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCaa                                                                                                                  >  1:840466/1‑68 (MQ=255)
                                                                                                aaCACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGcc                                >  1:473646/1‑150 (MQ=255)
                                                                                                      aTCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGc                          >  1:127482/1‑150 (MQ=255)
                                                                                                      aTCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGc                          >  1:1073626/1‑150 (MQ=255)
                                                                                                      aTCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGc                          <  2:51507/150‑1 (MQ=255)
                                                                                                      aTCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGc                          <  2:693003/150‑1 (MQ=255)
                                                                                                        cGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCga                        >  1:703250/1‑150 (MQ=255)
                                                                                                            aCCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGCGACAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCGAGAGc                    >  1:732534/1‑150 (MQ=255)
                                                                                                            aCCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCGAGAGc                    <  2:473646/150‑1 (MQ=255)
                                                                                                              ccAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCat                                                                 <  2:1214724/103‑1 (MQ=255)
                                                                                                              ccAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCat                                                                 >  1:1214724/1‑103 (MQ=255)
                                                                                                               cAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCGAGAGCGTg                 <  1:964565/150‑1 (MQ=255)
                                                                                                                aGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCGAGAGCGTgg                >  1:597216/1‑150 (MQ=255)
                                                                                                                       ggTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCGAGAGCGTGGCggcggg         <  2:210124/150‑1 (MQ=255)
                                                                                                                           tACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGAc                                   <  2:1257147/120‑1 (MQ=255)
                                                                                                                           tACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGCCAAGCTCGACCTTCTCGACAGACGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGAc                                   >  1:1257147/1‑120 (MQ=255)
                                                                                                                              aaGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAGGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACACGGATTACTGCATCACCACCGACGCCCTTGGCGAGAGCGTGGCGGCGGGCGAAgcg  >  1:1043029/1‑150 (MQ=255)
                                                                                                                                gCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAg                                                        <  2:653684/94‑1 (MQ=255)
                                                                                                                                gCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAg                                                        >  1:653684/1‑94 (MQ=255)
                                                                                                                                 ccTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATcacca                                        <  1:858812/109‑1 (MQ=255)
                                                                                                                                 ccTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATcacca                                        >  2:858812/1‑109 (MQ=255)
                                                                                                                                       aaGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCGAGAGCGTGGcggcgg          <  1:118918/133‑1 (MQ=255)
                                                                                                                                       aaGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCGAGAGCGTGGcggcgg          >  2:118918/1‑133 (MQ=255)
                                                                                                                                             |                                                                                                                                      
CAGGAAAAGGACGTTGCCACACCGCGCATCGGGGTGTTGTACCAACTGACCCCGCAAGTCGGCGTGTTCGCCAACGCATCCACTTCGTTCAAACCCAACACCATCGGCACCCAGGGCCAGGTGTACAAGCCTGAAAAGGGCTTGGGTTACGAGACCGGAGTCAAGCTCGACCTTCTCGACAGCCGCCTGGGCGCGACCATCGCCCTGTTCCATATCGACAAGGAGAACGTCATCACCACCGACGCCCTTGGCGAGAGCGTGGCGGCGGGCGAAGCG  >  NC_002947/3762067‑3762342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑