| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,763,549 | C→T | 8.2% | intergenic (+26/+1649) | PP_3326 → / ← PP_3328 | hypothetical protein/ring‑cleaving dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,763,549 | 0 | C | T | 8.2% | 168.6 / 5.0 | 61 | intergenic (+26/+1649) | PP_3326/PP_3328 | hypothetical protein/ring‑cleaving dioxygenase |
| Reads supporting (aligned to +/- strand): ref base C (25/31); new base T (3/2); total (28/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
CGCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCACTTGCATCACA > NC_002947/3763401‑3763698 | cGCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACg < 1:208032/150‑1 (MQ=255) aTGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCg < 1:16521/150‑1 (MQ=255) gAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCGTCATGGCCGAg < 2:1058816/150‑1 (MQ=255) aaGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCGTCATGGCCGAgg < 2:476825/150‑1 (MQ=255) aGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGt > 2:1000787/1‑150 (MQ=255) aCATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGt > 1:593190/1‑150 (MQ=255) catcatCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGtt < 1:1141043/150‑1 (MQ=255) atcatcGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCATCATAGCTTGTGCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTg < 2:495457/150‑1 (MQ=255) catcGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTTTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGa > 2:941768/1‑150 (MQ=255) atcGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAg < 1:570059/150‑1 (MQ=255) gCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTc < 1:455105/150‑1 (MQ=255) cccAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCg < 1:170735/150‑1 (MQ=255) aCCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGt < 2:99481/150‑1 (MQ=255) ccTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGt < 1:1076642/148‑1 (MQ=255) ccTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGt > 2:1076642/1‑148 (MQ=255) ccTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTCAGTATGCCATACCGCTAGTAAACTAAGCCACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGc < 1:797043/150‑1 (MQ=255) tGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCt < 2:823517/150‑1 (MQ=255) tGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCt < 2:257400/150‑1 (MQ=255) tGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCt > 1:823517/1‑150 (MQ=255) tGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGACGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCt > 1:269937/1‑150 (MQ=255) ggCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCa < 1:376657/150‑1 (MQ=255) cGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATcc < 1:178998/150‑1 (MQ=255) gTGGCCCAGCAGCGGCTGTTCCGTTCATCGTCACCCCGCGGTAAGCCGTTTCACTAGTAGACTATGCTACATACCTGGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGAc < 2:141269/150‑1 (MQ=255) gTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGAc < 2:503336/150‑1 (MQ=255) ccAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTg > 2:967386/1‑150 (MQ=255) cagcagAGCCTGTTAAGTTCCCTGTCGTTCCTGGGTAAGACGCACCACGAGTAAACTACGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTgg < 2:269937/150‑1 (MQ=255) gagCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACaa > 1:454665/1‑150 (MQ=255) gagCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACaa > 2:567995/1‑150 (MQ=255) tGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCg > 1:49536/1‑150 (MQ=255) cttcaCTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCg < 1:600201/149‑1 (MQ=255) caCTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGACTGCGTGCCTGCAACCAGCATCCACACCATCACCATTTGCACAAGCCCGGCTGCggag > 2:600201/1‑148 (MQ=255) aCTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCca > 2:832732/1‑150 (MQ=255) cTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCcac > 2:164496/1‑150 (MQ=255) gggTAAGCCGCACAAAGAGTAAACTAAGCGACATAGCTCGGCCATGACGGCCGAGGTATGACAGTTGCAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGa > 2:308551/1‑150 (MQ=255) ggTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCAt > 2:868921/1‑99 (MQ=255) ggTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCAt < 1:868921/99‑1 (MQ=255) cGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATg < 2:199772/150‑1 (MQ=255) gCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATgg > 2:663912/1‑150 (MQ=255) gAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCAtccc < 1:567995/150‑4 (MQ=255) gAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTc > 1:561075/1‑150 (MQ=255) gAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTc < 1:967386/150‑1 (MQ=255) aaCTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAac > 1:930874/1‑131 (MQ=255) aaCTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAac < 2:930874/131‑1 (MQ=255) tAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACCCGGCAATCTAAGGCGATGGGCATGTCGATTCCCt < 2:49536/150‑1 (MQ=255) tAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCt > 1:562029/1‑150 (MQ=255) caactgccATACCTCGGCCGTCATGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCt > 2:519378/8‑150 (MQ=39) gCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCa < 2:562029/150‑1 (MQ=255) cGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCat > 2:176279/1‑150 (MQ=255) gACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCatg > 2:641755/1‑150 (MQ=255) aCATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCatga > 1:1279799/1‑150 (MQ=255) aCATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGAGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCAtt < 2:107782/86‑1 (MQ=255) aCATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGAGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCAtt > 1:107782/1‑86 (MQ=255) aCCTCGGCCGTCATGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTAACTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATggg > 2:816107/1‑150 (MQ=255) aCCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGc > 1:158422/1‑85 (MQ=255) aCCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGc < 2:158422/85‑1 (MQ=255) aCCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATggg < 1:832732/150‑1 (MQ=255) ccTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGc < 1:941768/150‑1 (MQ=255) cTCGGCCGTCATGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCa > 1:480684/1‑150 (MQ=255) gCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCa < 2:367644/68‑1 (MQ=255) gCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCa > 1:367644/1‑68 (MQ=255) aTGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCAGTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCACTTGCAt < 1:663912/150‑1 (MQ=255) cGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCACTTGCATcaca < 1:164496/150‑1 (MQ=255) | CGCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCACTTGCATCACA > NC_002947/3763401‑3763698 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |