| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 247,393 | A→G | 6.0% | Q524R (CAG→CGG) | yheS → | ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 247,393 | 0 | A | G | 6.0% | 198.2 / 2.3 | 66 | Q524R (CAG→CGG) | yheS | ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (35/27); new base G (2/2); total (37/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
TTGCCGGTGCAGTGGTGGTGGTGTCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGAGCTCAACCA > NC_002947/247249‑247534 | ttGCCGGTGCAGTGGTGGTGGTGTCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCa < 1:48757/150‑1 (MQ=255) gCCGGTGCAGTGGTGGTGGTGTCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGAATTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGt > 1:48439/1‑150 (MQ=255) gtggtggtgTCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGt > 1:192355/1‑150 (MQ=255) gtCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCGGCGCACTGCGCTGGcggtac < 1:447583/143‑6 (MQ=255) gtCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCGGCGCACTGCGCTGGcggtac > 2:447583/1‑138 (MQ=255) tCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCg < 1:501397/150‑1 (MQ=255) ccACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGc > 2:870939/1‑150 (MQ=255) ccACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGc < 1:1273397/150‑1 (MQ=255) ccACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGc > 2:1279526/1‑150 (MQ=255) ccACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGc > 1:809030/1‑150 (MQ=255) ccACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGc < 2:1005066/150‑1 (MQ=255) cACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCa > 2:943867/1‑136 (MQ=255) cACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCa < 1:943867/136‑1 (MQ=255) tttGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACaa > 2:479368/1‑150 (MQ=255) tCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCTACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACAGCCAGCGCAGTGCGCCGGCCAGTACTTCACCGGCCAACCCGGACAAGACCg > 2:154558/1‑150 (MQ=255) tCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAgc < 2:176427/105‑1 (MQ=255) tCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAgc > 1:176427/1‑105 (MQ=255) tCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCg > 2:1210215/1‑150 (MQ=255) aaGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCg < 2:1279206/138‑1 (MQ=255) aaGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCg > 1:1279206/1‑138 (MQ=255) gCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGa > 2:536045/1‑150 (MQ=255) caccacTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTCCTGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGaa > 1:152403/1‑150 (MQ=255) cacTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCGGCGCACTGCGCTGGcgg > 1:924591/1‑108 (MQ=255) cacTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTg > 2:790095/1‑101 (MQ=255) cacTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTg < 1:790095/101‑1 (MQ=255) cacTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGc > 1:693040/1‑150 (MQ=255) cacTCATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTCCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCGGCGCACTGCGCTGGcgg < 2:924591/110‑3 (MQ=255) acTGATGACTTCCTGCTGGTGGCCGACGGCAAGGACTACACCTTCGACGGTGACCTGCAGGACTACACCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGcc > 1:930256/1‑150 (MQ=255) tgatgaCTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCa < 2:693040/150‑1 (MQ=255) gatgaCTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAg < 2:511069/150‑1 (MQ=255) atgaCTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAgc > 1:502150/1‑150 (MQ=255) cTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCa > 1:717303/1‑150 (MQ=255) ttCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAg < 2:809030/150‑1 (MQ=255) ttCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAg > 1:660471/1‑150 (MQ=255) tgctgGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCa > 1:364138/1‑84 (MQ=255) tgctgGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCa < 2:364138/84‑1 (MQ=255) tgctgGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTg < 2:723098/87‑1 (MQ=255) tgctgGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTg > 1:723098/1‑87 (MQ=255) tgctgGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAAccc < 2:1068883/115‑1 (MQ=255) tgctgGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAAccc > 1:1068883/1‑115 (MQ=255) gCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGa > 1:217233/1‑110 (MQ=255) gCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGa < 2:217233/110‑1 (MQ=255) cGACGGCAAGGTCGCCACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACACGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCtt > 2:604542/1‑150 (MQ=255) ggTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTcagca < 2:502150/150‑1 (MQ=255) tCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGt < 1:479368/150‑1 (MQ=255) ccaacacCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGt < 2:930256/147‑1 (MQ=255) cTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCAcc > 2:1285569/1‑150 (MQ=255) ttCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCg > 1:1129880/1‑150 (MQ=255) cGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGAAGTTGGCACCGca < 2:660471/150‑1 (MQ=255) ccTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGc < 2:1129880/150‑1 (MQ=255) cTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCt > 2:196348/1‑150 (MQ=255) aCTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGACAGCCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAGGGCTGCCGACa > 1:288754/1‑150 (MQ=255) tACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGaaa < 2:247671/84‑1 (MQ=255) tACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGaaa > 1:247671/1‑84 (MQ=255) aCAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGc < 1:870939/150‑1 (MQ=255) aGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCg > 1:538644/1‑54 (MQ=255) aGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCg < 2:538644/54‑1 (MQ=255) aGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTg < 1:536045/150‑1 (MQ=255) tgcGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGCCACCGCAAGAGAAGGCGGGCGAGGAGCTgg > 2:73703/3‑150 (MQ=255) gcttgTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGa > 2:638717/1‑150 (MQ=255) ctggTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGAg < 1:196348/150‑1 (MQ=255) aGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTg < 1:1249505/92‑1 (MQ=255) aGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTg > 2:1249505/1‑92 (MQ=255) gTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGAGCTCAACCa > 2:1150178/1‑150 (MQ=255) gCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACaagaa < 1:1170948/112‑1 (MQ=255) gCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACaagaa > 2:1170948/1‑112 (MQ=255) | TTGCCGGTGCAGTGGTGGTGGTGTCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGCCGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGAGCTCAACCA > NC_002947/247249‑247534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |