Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,960,565 A→T 12.2% intergenic (‑137/‑192) mnxG ← / → PP_3491 manganese‑oxidizing multicopper oxidase/enoly‑coenzyme A hydratase/isomerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,960,5650AT12.2% 149.0 / 11.4 56intergenic (‑137/‑192)mnxG/PP_3491manganese‑oxidizing multicopper oxidase/enoly‑coenzyme A hydratase/isomerase family protein
Reads supporting (aligned to +/- strand):  ref base A (23/26);  new base T (3/4);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CGGCGTCGACATGCTGCACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAA  >  NC_002947/3960417‑3960710
                                                                                                                                                    |                                                                                                                                                 
cggcgTCGACATGCTGCACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAAt                                                                                                                                                  >  2:968597/1‑150 (MQ=255)
   cgTCGACATGCTGCACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCgg                                                                                                                                               <  2:476827/150‑1 (MQ=255)
    gTCGACATGCTGCACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGATTCggg                                                                                                                                              <  1:14127/150‑1 (MQ=255)
    gTCGACATGCTGCACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCggg                                                                                                                                              <  2:1171745/150‑1 (MQ=255)
        aCATGCTGCACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGAc                                                                                                                                          <  2:1265163/150‑1 (MQ=255)
         cATGCTGCACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAACAGTTCAATATTAATCACTAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGATTCGGTGGACg                                                                                                                                         <  2:403936/150‑1 (MQ=255)
           tgctgcACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGa                                                                                                                                       >  1:711137/1‑150 (MQ=255)
                cACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATccc                                                                                                                                  >  2:1053604/1‑150 (MQ=255)
                   cTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGATTCGGGGGaa                                                                                                                                          >  1:858769/1‑138 (MQ=255)
                   cTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAACGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGATTCGGGGGaa                                                                                                                                          <  2:858769/139‑2 (MQ=255)
                   cTCCGGGACAAAGGATCTAGGCGGGGAGCTAGAGCAAGGTGTGTGAGAGAAGAAAAGTTCAATATTAATCAATAACGTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTg                                                                                                                               <  2:224114/150‑1 (MQ=255)
                    tCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCgg                                                                                                                                               <  1:1201105/133‑1 (MQ=255)
                    tCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCgg                                                                                                                                               >  2:1201105/1‑133 (MQ=255)
                        ggACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGc                                                                                                                          <  2:693209/150‑1 (MQ=255)
                           cacaGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCaaa                                                                                                                       <  1:1053604/150‑1 (MQ=255)
                            acaGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAg                                                                                                                      >  2:528365/1‑150 (MQ=255)
                              aGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGgc                                                                                                                    >  1:167513/1‑150 (MQ=255)
                                   ctctGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTc                                                                                                               <  1:968597/150‑1 (MQ=255)
                                      tGCGGGGAGCTGGAGAAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCcac                                                                                                            <  1:653802/150‑1 (MQ=255)
                                        cGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACaa                                                                                                          <  2:1167563/150‑1 (MQ=255)
                                                tGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAgg                                                                                                                     >  1:1061135/1‑131 (MQ=255)
                                                tGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAgg                                                                                                                     <  2:1061135/131‑1 (MQ=255)
                                                tGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGgctg                                                                                                                  <  2:867557/134‑1 (MQ=255)
                                                tGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGgctg                                                                                                                  >  1:867557/1‑134 (MQ=255)
                                                     cAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATc                                                                                                                                                 >  2:803373/1‑98 (MQ=255)
                                                     cAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATc                                                                                                                                                 <  1:803373/98‑1 (MQ=255)
                                                       aGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTc                                                                                           <  1:889002/150‑1 (MQ=255)
                                                              tgCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAg                                                                                    <  2:167513/150‑1 (MQ=255)
                                                                  agaagaAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTgggg                                                                                                                            <  2:406610/106‑1 (MQ=255)
                                                                  agaagaAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTgggg                                                                                                                            >  1:406610/1‑106 (MQ=255)
                                                                  agaagaAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTaa                                                                                >  2:1126845/1‑150 (MQ=255)
                                                                  agaagaAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTaa                                                                                >  2:484184/1‑150 (MQ=255)
                                                                            ttCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACgg                                                                      >  1:1024443/1‑150 (MQ=255)
                                                                              cAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTc                                                                                                               >  2:1075279/1‑107 (MQ=255)
                                                                              cAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTc                                                                                                               <  1:1075279/107‑1 (MQ=255)
                                                                               aaTATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTAc                                                                   <  2:711137/150‑1 (MQ=255)
                                                                                           tAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAAtt                                                       <  2:725474/150‑1 (MQ=255)
                                                                                                tGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGa                                                  <  1:528365/150‑1 (MQ=255)
                                                                                                      aTCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGc                                            >  1:638683/1‑150 (MQ=255)
                                                                                                        cAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCaa                                          <  1:579633/150‑1 (MQ=255)
                                                                                                            cGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCGAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCa                                      >  1:845223/1‑150 (MQ=255)
                                                                                                                        gggAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGcc                          >  2:660155/1‑150 (MQ=255)
                                                                                                                            gcaTCCCCCAGCCGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAAGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTTCACAATAGTCGATTGTGCACGACAAATTCGCATGCCAGGa                      >  2:214638/3‑150 (MQ=255)
                                                                                                                                 ccccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGc                                                             <  2:730347/106‑1 (MQ=255)
                                                                                                                                 ccccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGc                                                             >  1:730347/1‑106 (MQ=255)
                                                                                                                                      gcagGTTCCCCCGAATCGGCGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGAtc            >  1:388786/1‑150 (MQ=255)
                                                                                                                                        tccgtCCCCCGATTCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGAtcct          >  1:1212684/5‑150 (MQ=255)
                                                                                                                                         ggTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGAtcctc         <  1:660155/150‑1 (MQ=255)
                                                                                                                                         ggTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGAtcctc         >  1:1101335/1‑150 (MQ=255)
                                                                                                                                         ggTTCCCCCGAATCGGGGGACGGAATCCCGTGCGATTAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGAtcctc         <  2:622007/150‑1 (MQ=255)
                                                                                                                                           gtCCCCCGATTCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTg                 >  1:447640/2‑140 (MQ=255)
                                                                                                                                           gtCCCCCGATTCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTg                 <  2:447640/139‑1 (MQ=255)
                                                                                                                                               cccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGa                      <  2:555907/131‑1 (MQ=255)
                                                                                                                                               cccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGa                      >  1:555907/1‑131 (MQ=255)
                                                                                                                                                ccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGaa  >  2:534594/1‑150 (MQ=255)
                                                                                                                                                ccGAATCGGGGGACGGAATCCCGGGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACCCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCACCCGCTaa  <  2:894095/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGGCGTCGACATGCTGCACCTCCGGGACACAGGATCTCTGCGGGGAGCTGGAGCAAGGTGTGTGCCAGAAGAAAAGTTCAATATTAATCAATAACTTGTACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAA  >  NC_002947/3960417‑3960710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑