| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,143,926 | T→A | 10.5% | intergenic (+35/‑88) | PP_3647 → / → PP_3648 | oxidoreductase/carboxymuconolactone decarboxylase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,143,926 | 0 | T | A | 10.5% | 157.6 / 10.5 | 57 | intergenic (+35/‑88) | PP_3647/PP_3648 | oxidoreductase/carboxymuconolactone decarboxylase family protein |
| Reads supporting (aligned to +/- strand): ref base T (26/25); new base A (4/2); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 | |||||||||||
GCGATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTGGGCGAGGACTA > NC_002947/4143787‑4144075 | cgatctaTTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTGGGCATCTTC‑GCGGGCAAGCCCGCGaa < 2:223679/144‑1 (MQ=255) gATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCAAGCCCGCGAAGa > 1:501264/1‑150 (MQ=255) aTCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCAAGCCCGCGAAGAt < 2:501264/150‑1 (MQ=255) aTTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGcc < 2:206782/150‑1 (MQ=255) ttGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGccc > 2:1130363/1‑150 (MQ=255) ttGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCAGATGACGCAAATCGTGTACCACAAGGGCAAGCTCGTCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTGGGCATCTTC‑GCGGGCAAGCCCGCGAAGagatcg > 1:223679/1‑145 (MQ=255) gcgcGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGttt > 2:146379/1‑150 (MQ=255) cgGTGAAGAACCTGGTGTGACCTGAGCAGCGGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCa < 2:1257804/150‑1 (MQ=255) tGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTAGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAggg > 1:962956/1‑150 (MQ=255) gaagaaCCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAgggg > 2:1038532/1‑150 (MQ=255) aagaaCCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGt < 2:760148/150‑1 (MQ=255) gaaCCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GAGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTcg > 2:769582/1‑150 (MQ=255) ccTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCgcgc < 1:146379/150‑1 (MQ=255) cTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCt > 1:529787/1‑150 (MQ=255) gtTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCg > 2:97119/1‑150 (MQ=255) ttGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGccc > 2:269780/1‑124 (MQ=255) ttGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGccc < 1:269780/124‑1 (MQ=255) tGCCTGAGCTGCAGATGACCCCTATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTCAGCCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGtc < 1:265541/150‑1 (MQ=255) cTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCa > 2:957087/1‑150 (MQ=255) cTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCa > 1:991408/1‑150 (MQ=255) cagcCGATGACCCCGATGGTGTACCACCAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTc > 1:997664/1‑150 (MQ=255) agcCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCg < 2:962956/150‑1 (MQ=255) ccGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGt > 2:124996/1‑150 (MQ=255) aTGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGc < 1:124996/150‑1 (MQ=255) aCCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GAGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGc < 1:769582/150‑1 (MQ=255) cccGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGccc > 2:411373/1‑150 (MQ=255) ccGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCgg < 2:1070802/140‑1 (MQ=255) ccGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCgg > 1:1070802/1‑140 (MQ=255) ccGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGcccc > 1:201667/1‑150 (MQ=255) ggTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCttttt < 2:1153074/150‑1 (MQ=255) tgtACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGAt < 2:605653/92‑1 (MQ=255) tgtACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGAt > 1:605653/1‑92 (MQ=255) gtACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTAt > 2:1023944/1‑150 (MQ=255) tACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATg > 1:970069/1‑150 (MQ=255) cacaAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCg < 1:1038532/150‑1 (MQ=255) cacaAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCg > 2:1025087/1‑150 (MQ=255) cacaAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCg > 1:850056/1‑150 (MQ=255) acaAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCgg > 1:657824/1‑150 (MQ=255) caAGTGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGa < 2:529787/150‑1 (MQ=255) caAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGa < 2:850056/150‑1 (MQ=255) caAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGa < 1:957087/150‑1 (MQ=255) gggCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTTCAGCCCCTTTTTTATGCCCTAGTa > 1:346785/1‑150 (MQ=255) cAAGCTCGGCGCCCTGCCGCCAATTGGCATATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTg > 1:998718/1‑150 (MQ=255) ggCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGa < 1:97119/150‑1 (MQ=255) cccTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCaa < 1:1025087/150‑1 (MQ=255) ccTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTACGGTGATCTTG‑CCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAAt < 2:997664/150‑1 (MQ=255) aCTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATg > 2:261760/1‑118 (MQ=255) aCTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATg < 1:261760/118‑1 (MQ=255) cTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACg > 2:644765/1‑150 (MQ=255) gCTGATACATCTTGACTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGaaaa < 1:408412/150‑1 (MQ=255) tGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAgg < 2:131933/150‑1 (MQ=255) aTACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGc < 2:201667/150‑1 (MQ=255) ttGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAgg < 2:408769/141‑1 (MQ=255) ttGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAgg > 1:408769/1‑141 (MQ=255) cATCTTC‑GCGGGCAAGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTAAGGAAAGTGCCTGAAAATCCGCACCCAGGTAc > 2:802534/1‑150 (MQ=255) ttC‑GCGGGCAAGCACGAGCAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTggg > 2:633538/1‑150 (MQ=255) cgGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAACTCCGCACCCAGGTACTGGGCGAg < 2:1244482/150‑1 (MQ=255) ttGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTGGGCGAGGACTa > 1:67391/1‑150 (MQ=255) | GCGATCATTGTCGGCGCGGTGAAGAACCTGGTGTTGCCTGAGCAGCCGATGACCCCGATGGTGTACCACAAGGGCAAGCTCGGCGCCCTGCCGCCACTTGGCTGATACATCTTGAGTCGATGTTGGCATCTTC‑GCGGGCTTGCCCGCGAAGATGCCCCTGTTTTCAGGGGTCGCGCTAATCGTCTCACCCTCGGTTGCGGCCCCTTTTTTATGCCGGAGTACTGCACATGAGCAATGAAAAGTACGAAAAGGGCCTGAAAATCCGCACCCAGGTACTGGGCGAGGACTA > NC_002947/4143787‑4144075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |