Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,160,705 G→C 8.0% intergenic (+27/+94) PP_3662 → / ← PP_3663 hypothetical protein/GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,160,7050GC 8.0% 154.0 / 5.4 51intergenic (+27/+94)PP_3662/PP_3663hypothetical protein/GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (23/23);  new base C (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

ACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGCCCC  >  NC_002947/4160560‑4160853
                                                                                                                                                 |                                                                                                                                                    
aCGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATg                                                                                                                                                  >  1:985855/1‑150 (MQ=255)
   gTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGccc                                                                                                                                               <  1:1095045/150‑1 (MQ=255)
   gTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGccc                                                                                                                                               <  2:1116558/150‑1 (MQ=255)
    tGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGacggcga                                                                                                                                              <  2:561068/150‑7 (MQ=255)
         gcgcCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGTcctc                                                                                                                                         <  1:648522/150‑4 (MQ=255)
           gcCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAAcg                                                                                                                                       >  1:1252430/1‑150 (MQ=255)
             cGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGAATGCCCGTCGAAcgcg                                                                                                                                     >  1:411005/1‑150 (MQ=255)
                    gACCCGCTAAGCCGGCTGCTGCAGGCATTCGTCGACGAGCACTGGATGAAGCTGCCCTGCGGCTCGGCTTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCg                                                                                                                              >  2:531875/1‑150 (MQ=255)
                       ccGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGt                                                                                                                           <  2:907300/150‑1 (MQ=255)
                               ccGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAg                                                                                                                   >  1:964747/1‑150 (MQ=255)
                               ccGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAg                                                                                                                   >  2:370609/1‑150 (MQ=255)
                                        gcAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTAc                                                                                                          <  1:673123/150‑1 (MQ=255)
                                         cAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACg                                                                                                         >  2:293258/1‑150 (MQ=255)
                                          aGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACgg                                                                                                        >  1:98403/1‑150 (MQ=255)
                                                    cgACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGAATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCcaca                                                                                              <  2:411005/150‑1 (MQ=255)
                                                     gACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACAt                                                                                             <  2:964747/150‑1 (MQ=255)
                                                      aCCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACTGGCATCCATGCCCGTc                                                                                                                                            <  2:826493/102‑1 (MQ=255)
                                                      aCCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGTc                                                                                                                                            >  1:826493/1‑102 (MQ=255)
                                                      aCCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTCATCTACTCCCGACGGGAATTTAGTCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATg                                                                                            <  1:1120944/150‑1 (MQ=255)
                                                         aGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCgg                                                                                                                             >  1:433861/1‑114 (MQ=255)
                                                         aGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCgg                                                                                                                             <  2:433861/114‑1 (MQ=255)
                                                              cGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCcaca                                                                                              <  1:78209/140‑1 (MQ=255)
                                                              cGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCcaca                                                                                              >  2:78209/1‑140 (MQ=255)
                                                                      gCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTc                                                                            >  2:130991/1‑150 (MQ=255)
                                                                      gCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTc                                                                            >  2:1075629/1‑150 (MQ=255)
                                                                            cggcggCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCAc                                                                      <  1:293258/150‑1 (MQ=255)
                                                                            accctgcgcggcgTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACACACATGGGTTACATGAAAGCTCCCCCAc                                                                      >  1:44277/6‑150 (MQ=255)
                                                                             ggcggcGCGGCGTATGTACCGTGCTATCAGGTGGCAACCCGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCAcc                                                                     <  1:8355/150‑1 (MQ=255)
                                                                              gcggcgcggcGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGAc                                                                                                                        <  1:111818/98‑1 (MQ=255)
                                                                              gcggcgcggcGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGAc                                                                                                                        >  2:111818/1‑98 (MQ=255)
                                                                                 gcgcggcgTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAgg                                                                 <  2:985855/150‑1 (MQ=255)
                                                                                     ggcgTATGTACCGTGCTATCAGGTGGCGACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGAc                                                             <  2:91413/150‑1 (MQ=255)
                                                                                           tGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATg                                                                                                                                                      <  1:321299/55‑1 (MQ=255)
                                                                                           tGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATg                                                                                                                                                      >  2:321299/1‑55 (MQ=255)
                                                                                                       tCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACt                                                    >  1:3827/1‑141 (MQ=255)
                                                                                                       tCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACt                                                    <  2:3827/141‑1 (MQ=255)
                                                                                                         aGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCa                                         >  2:367162/1‑150 (MQ=255)
                                                                                                          ggTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCaa                                        <  1:367162/150‑1 (MQ=255)
                                                                                                            tGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAacc                                      <  1:370609/150‑1 (MQ=255)
                                                                                                            tGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAacc                                      <  2:733161/150‑1 (MQ=255)
                                                                                                               cAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAaccacc                                   <  1:531875/150‑1 (MQ=255)
                                                                                                                aaCCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGaaa                                                                                >  2:1230116/1‑104 (MQ=255)
                                                                                                                aaCCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGaaa                                                                                <  1:1230116/104‑1 (MQ=255)
                                                                                                                 aCCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCAccgc                                 >  1:1123080/1‑150 (MQ=255)
                                                                                                                          gctgctTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGaa                        >  1:234073/1‑150 (MQ=255)
                                                                                                                             gctTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGaaaaa                     >  2:80424/1‑150 (MQ=255)
                                                                                                                                  aCTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACa                                                                                     >  1:216895/1‑81 (MQ=255)
                                                                                                                                  aCTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACa                                                                                     <  2:216895/81‑1 (MQ=255)
                                                                                                                                    tGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCca                                                                                                <  1:117265/68‑1 (MQ=255)
                                                                                                                                    tGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCca                                                                                                >  2:117265/1‑68 (MQ=255)
                                                                                                                                        gACGGGCATCCATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGt          >  2:1266941/1‑150 (MQ=255)
                                                                                                                                               aTGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGccc   >  1:204826/1‑150 (MQ=255)
                                                                                                                                                tGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTCGCTGCCGCTCTCACTGCAGGCGTTGAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGcccc  <  2:44277/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGCCCC  >  NC_002947/4160560‑4160853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑