Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,241,017 A→G 8.1% I242I (ATT→ATC) ‡ PP_3718 ← aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,241,0170AG 8.1% 173.3 / 4.9 62I242I (ATT→ATC) ‡PP_3718aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (25/31);  new base G (3/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

GTGATGATGTCTGGGGTGACGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTT  >  NC_002947/4240868‑4241166
                                                                                                                                                     |                                                                                                                                                     
gtgATGATGTCTGGGGTGACGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGa                                                                                                                                                       >  1:277321/1‑150 (MQ=255)
 tgatgaTGTCTGGGGTGACGCCAAACAGTTCCTCGCTGGAGAAGAAGAAGCCCAGACGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGaa                                                                                                                                                      <  2:144681/150‑1 (MQ=255)
         tCTGGGGTGACGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCAcc                                                                                                                                              >  2:1086054/1‑150 (MQ=255)
                 gACGCCAAACAGTTCCTCGCTGGCGCAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACCGGCTGTCGTAGGTCTGGCACTGCTTTCACGTTCTCTTGAATTAGCCCTCGGGCGGAATGATCACCCCGCCCGa                                                                                                                                      <  1:543258/150‑1 (MQ=255)
                   cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGGGTGATCATTCCGCCCGa                                                                                                                                      >  2:823261/1‑148 (MQ=255)
                   cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGGGTGATCATTCCGCCCGa                                                                                                                                      <  1:823261/148‑1 (MQ=255)
                   cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAgg                                                                                                                                    <  2:707260/150‑2 (MQ=255)
                   cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGc                                                                                                                                    <  2:590972/150‑1 (MQ=255)
                   cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGc                                                                                                                                    <  1:971430/150‑1 (MQ=255)
                    gCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGcc                                                                                                                                   >  1:338129/1‑150 (MQ=255)
                     ccAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGccc                                                                                                                                  >  1:680510/1‑150 (MQ=255)
                     ccAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGccc                                                                                                                                  <  1:1166817/150‑1 (MQ=255)
                       aaaCAGTTCCTTGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGGACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCAt                                                                                                                                >  2:1130915/1‑150 (MQ=255)
                           aGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAtc                                                                                                                            <  2:1240997/150‑1 (MQ=255)
                                cTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAtcggctc                                                                                                                       <  1:1258360/150‑1 (MQ=255)
                                  cGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAtcggctcgg                                                                                                                     <  1:1236259/150‑1 (MQ=255)
                                    cTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGACCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCg                                                                                                                   >  2:1113290/1‑150 (MQ=255)
                                       gCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCCACCCGCCCGAGCTCGTGATCGGCTCGGCgaag                                                                                                                >  1:706917/1‑150 (MQ=255)
                                         gaagaaGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCgaagaa                                                                                                              >  2:529244/1‑150 (MQ=255)
                                             aagGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                          <  1:630118/150‑1 (MQ=255)
                                               ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGGGTGATCATTCCGCCCGAg                                                                                                                                     >  1:1085446/1‑121 (MQ=255)
                                               ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGGGTGATCATTCCGCCCGAg                                                                                                                                     <  2:1085446/121‑1 (MQ=255)
                                               ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgc                                                                                                        >  2:1141067/1‑150 (MQ=255)
                                               ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                          <  2:661575/148‑1 (MQ=255)
                                               ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTAGGCGAAGAATtcc                                                                                                          >  1:661575/1‑148 (MQ=255)
                                               ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCATCGGTGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgc                                                                                                        >  1:622505/1‑150 (MQ=255)
                                                   cccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACcccgccc                                                                                                                                        >  2:671396/1‑114 (MQ=255)
                                                   cccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACcccgccc                                                                                                                                        <  1:671396/114‑1 (MQ=255)
                                                    ccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgcc                                                                                                       >  2:699331/1‑146 (MQ=255)
                                                    ccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgcc                                                                                                       <  1:699331/146‑1 (MQ=255)
                                                      aGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTg                                                                                                 >  2:43207/1‑150 (MQ=255)
                                                         cgcCCGAATGAGGTCCTGACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACCTGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCg                                                                                              <  2:277321/150‑1 (MQ=255)
                                                          gcCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGACGAATGCCGCCACGTTGTCgg                                                                                             >  2:399324/1‑150 (MQ=255)
                                                             cGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCAc                                                                                          <  1:1164695/150‑1 (MQ=255)
                                                                  gAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACACAGc                                                                                     <  1:1086054/150‑1 (MQ=255)
                                                                      tCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                          >  2:596654/1‑125 (MQ=255)
                                                                      tCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                          <  1:596654/125‑1 (MQ=255)
                                                                        accacTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAgg                                                                               >  1:1011943/1‑150 (MQ=255)
                                                                              tcgtcgGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTg                                                                         <  1:879559/150‑1 (MQ=255)
                                                                                  cgGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGCCCt                                                                     <  2:680510/150‑1 (MQ=255)
                                                                                   ggCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTc                                                                    <  1:529244/150‑1 (MQ=255)
                                                                                        cgaACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACt                                                               <  1:426846/150‑1 (MQ=255)
                                                                                          aaCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCg                                                             <  2:46230/150‑1 (MQ=255)
                                                                                          aaCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCg                                                             <  1:556923/150‑1 (MQ=255)
                                                                                           aCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCCCTCGCATCAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCgg                                                            <  1:726109/150‑1 (MQ=255)
                                                                                               gATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGccac                                                        >  2:1125518/1‑150 (MQ=255)
                                                                                                     gTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                          >  2:121485/1‑94 (MQ=255)
                                                                                                     gTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                          <  1:121485/94‑1 (MQ=255)
                                                                                                     gTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGaa                                                  <  2:323387/150‑1 (MQ=255)
                                                                                                      tAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAACCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgc                                                                                                           <  2:842221/92‑1 (MQ=255)
                                                                                                      tAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAACCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgc                                                                                                           >  1:842221/1‑92 (MQ=255)
                                                                                                           cTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAg                                            <  1:43207/150‑1 (MQ=255)
                                                                                                                acaGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACt                                       >  1:1035069/1‑150 (MQ=255)
                                                                                                                    cTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgcc                                                                                                       >  2:579342/1‑82 (MQ=255)
                                                                                                                    cTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgcc                                                                                                       <  1:579342/82‑1 (MQ=255)
                                                                                                                    cTGCCACATGCGCTGGAAGTAGCCCTAGGGCGGCATGATCACCCCGCGCGAGCCCATGATCAGCTCGGCTGAGTATGCCGCCACGTTATCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAATCGAGGGACTACGc                                   >  1:414580/1‑150 (MQ=255)
                                                                                                                       ccACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTc                                >  1:762190/1‑150 (MQ=255)
                                                                                                                           aTGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTcc                            <  2:762190/150‑1 (MQ=255)
                                                                                                                                       tAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGgc                <  2:1035069/150‑1 (MQ=255)
                                                                                                                                           gcTCGGGCGGGGTGATCATTCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGgcgcgc            >  2:104696/2‑150 (MQ=255)
                                                                                                                                            cTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCt                   >  2:308045/1‑142 (MQ=255)
                                                                                                                                            cTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCt                   <  1:308045/142‑1 (MQ=255)
                                                                                                                                                ggCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTAc       >  1:491431/1‑150 (MQ=255)
                                                                                                                                                   ggAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCAGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACggg    >  1:1048850/1‑150 (MQ=255)
                                                                                                                                                     aaTGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGGCGACACGTTGTCGGCACCCAGCGAGAGGATCTTGTCATCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggtt  >  1:615123/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGATGATGTCTGGGGTGACGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTT  >  NC_002947/4240868‑4241166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑