Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,241,018 A→G 8.1% I242T (ATT→ACT) ‡ PP_3718 ← aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,241,0180AG 8.1% 174.8 / 5.0 62I242T (ATT→ACT) ‡PP_3718aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (26/31);  new base G (3/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

TGATGATGTCTGGGGTGACGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTG  >  NC_002947/4240869‑4241167
                                                                                                                                                     |                                                                                                                                                     
tgatgaTGTCTGGGGTGACGCCAAACAGTTCCTCGCTGGAGAAGAAGAAGCCCAGACGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGaa                                                                                                                                                       <  2:144681/150‑1 (MQ=255)
        tCTGGGGTGACGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCAcc                                                                                                                                               >  2:1086054/1‑150 (MQ=255)
                gACGCCAAACAGTTCCTCGCTGGCGCAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACCGGCTGTCGTAGGTCTGGCACTGCTTTCACGTTCTCTTGAATTAGCCCTCGGGCGGAATGATCACCCCGCCCGa                                                                                                                                       <  1:543258/150‑1 (MQ=255)
                  cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGGGTGATCATTCCGCCCGa                                                                                                                                       >  2:823261/1‑148 (MQ=255)
                  cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGGGTGATCATTCCGCCCGa                                                                                                                                       <  1:823261/148‑1 (MQ=255)
                  cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAgg                                                                                                                                     <  2:707260/150‑2 (MQ=255)
                  cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGc                                                                                                                                     <  2:590972/150‑1 (MQ=255)
                  cGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGc                                                                                                                                     <  1:971430/150‑1 (MQ=255)
                   gCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGcc                                                                                                                                    >  1:338129/1‑150 (MQ=255)
                    ccAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGccc                                                                                                                                   >  1:680510/1‑150 (MQ=255)
                    ccAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGccc                                                                                                                                   <  1:1166817/150‑1 (MQ=255)
                      aaaCAGTTCCTTGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGGACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCAt                                                                                                                                 >  2:1130915/1‑150 (MQ=255)
                          aGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAtc                                                                                                                             <  2:1240997/150‑1 (MQ=255)
                               cTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAtcggctc                                                                                                                        <  1:1258360/150‑1 (MQ=255)
                                 cGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGAtcggctcgg                                                                                                                      <  1:1236259/150‑1 (MQ=255)
                                   cTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGACCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCg                                                                                                                    >  2:1113290/1‑150 (MQ=255)
                                      gCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCCACCCGCCCGAGCTCGTGATCGGCTCGGCgaag                                                                                                                 >  1:706917/1‑150 (MQ=255)
                                        gaagaaGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCgaagaa                                                                                                               >  2:529244/1‑150 (MQ=255)
                                            aagGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                           <  1:630118/150‑1 (MQ=255)
                                              ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGGGTGATCATTCCGCCCGAg                                                                                                                                      <  2:1085446/121‑1 (MQ=255)
                                              ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGGGTGATCATTCCGCCCGAg                                                                                                                                      >  1:1085446/1‑121 (MQ=255)
                                              ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgc                                                                                                         >  2:1141067/1‑150 (MQ=255)
                                              ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                           <  2:661575/148‑1 (MQ=255)
                                              ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTAGGCGAAGAATtcc                                                                                                           >  1:661575/1‑148 (MQ=255)
                                              ggTGCCCAGGCGCCCGAACGAGGTCACCACTTCATCGGTGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgc                                                                                                         >  1:622505/1‑150 (MQ=255)
                                                  cccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACcccgccc                                                                                                                                         >  2:671396/1‑114 (MQ=255)
                                                  cccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACcccgccc                                                                                                                                         <  1:671396/114‑1 (MQ=255)
                                                   ccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgcc                                                                                                        >  2:699331/1‑146 (MQ=255)
                                                   ccAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgcc                                                                                                        <  1:699331/146‑1 (MQ=255)
                                                     aGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTg                                                                                                  >  2:43207/1‑150 (MQ=255)
                                                        cgcCCGAATGAGGTCCTGACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACCTGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCg                                                                                               <  2:277321/150‑1 (MQ=255)
                                                         gcCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGACGAATGCCGCCACGTTGTCgg                                                                                              >  2:399324/1‑150 (MQ=255)
                                                            cGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCAc                                                                                           <  1:1164695/150‑1 (MQ=255)
                                                                 gAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACACAGc                                                                                      <  1:1086054/150‑1 (MQ=255)
                                                                     tCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                           <  1:596654/125‑1 (MQ=255)
                                                                     tCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                           >  2:596654/1‑125 (MQ=255)
                                                                       accacTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAgg                                                                                >  1:1011943/1‑150 (MQ=255)
                                                                             tcgtcgGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTg                                                                          <  1:879559/150‑1 (MQ=255)
                                                                                 cgGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGCCCt                                                                      <  2:680510/150‑1 (MQ=255)
                                                                                  ggCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTc                                                                     <  1:529244/150‑1 (MQ=255)
                                                                                       cgaACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACt                                                                <  1:426846/150‑1 (MQ=255)
                                                                                         aaCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCg                                                              <  1:556923/150‑1 (MQ=255)
                                                                                         aaCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCg                                                              <  2:46230/150‑1 (MQ=255)
                                                                                          aCAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCCCTCGCATCAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCgg                                                             <  1:726109/150‑1 (MQ=255)
                                                                                              gATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGccac                                                         >  2:1125518/1‑150 (MQ=255)
                                                                                                    gTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                           <  1:121485/94‑1 (MQ=255)
                                                                                                    gTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgcc                                                                                                           >  2:121485/1‑94 (MQ=255)
                                                                                                    gTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGaa                                                   <  2:323387/150‑1 (MQ=255)
                                                                                                     tAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAACCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgc                                                                                                            <  2:842221/92‑1 (MQ=255)
                                                                                                     tAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAACCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgc                                                                                                            >  1:842221/1‑92 (MQ=255)
                                                                                                          cTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAg                                             <  1:43207/150‑1 (MQ=255)
                                                                                                               acaGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACt                                        >  1:1035069/1‑150 (MQ=255)
                                                                                                                   cTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgcc                                                                                                        >  2:579342/1‑82 (MQ=255)
                                                                                                                   cTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATgccgcc                                                                                                        <  1:579342/82‑1 (MQ=255)
                                                                                                                   cTGCCACATGCGCTGGAAGTAGCCCTAGGGCGGCATGATCACCCCGCGCGAGCCCATGATCAGCTCGGCTGAGTATGCCGCCACGTTATCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAATCGAGGGACTACGc                                    >  1:414580/1‑150 (MQ=255)
                                                                                                                      ccACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTc                                 >  1:762190/1‑150 (MQ=255)
                                                                                                                          aTGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTcc                             <  2:762190/150‑1 (MQ=255)
                                                                                                                                      tAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGgc                 <  2:1035069/150‑1 (MQ=255)
                                                                                                                                          gcTCGGGCGGGGTGATCATTCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGgcgcgc             >  2:104696/2‑150 (MQ=255)
                                                                                                                                           cTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCt                    >  2:308045/1‑142 (MQ=255)
                                                                                                                                           cTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCt                    <  1:308045/142‑1 (MQ=255)
                                                                                                                                               ggCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTAc        >  1:491431/1‑150 (MQ=255)
                                                                                                                                                  ggAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCAGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACggg     >  1:1048850/1‑150 (MQ=255)
                                                                                                                                                    aaTGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGGCGACACGTTGTCGGCACCCAGCGAGAGGATCTTGTCATCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggtt   >  1:615123/1‑150 (MQ=255)
                                                                                                                                                     aTGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggttg  >  1:525983/1‑150 (MQ=255)
                                                                                                                                                     aTGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACgggttg  <  2:706917/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGATGATGTCTGGGGTGACGCCAAACAGTTCCTCGCTGGCGAAGAAGGTGCCCAGGCGCCCGAACGAGGTCACCACTTCGTCGGCGACGAACAGGATGTCGTAGGTCTGGCACAGCTGCCACATGCGCTGGAAGTAGCCCTCGGGCGGAATGATCACCCCGCCCGAGCCCATGATCGGCTCGGCGAAGAATGCCGCCACGTTGTCGGCACCCAGCGAGAGGATCTTGTCCTCGAACTCGGCCACCAGGAAGTCGAGGAACTCCGCCTCGTCCATGTCGTCTGGCGCGCGGTACGGGTTG  >  NC_002947/4240869‑4241167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑