Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 271,282 C→T 7.1% Q395* (CAG→TAG)  PP_0218 → sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947271,2820CT 7.1% 159.0 / 2.1 56Q395* (CAG→TAG) PP_0218sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (26/26);  new base T (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

TGCCGAGCAGTTGCTGCGCAATGCCGACTCGGCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGTGAGTTCATCCCGATTGCCGAACGCAC  >  NC_002947/271134‑271424
                                                                                                                                                    |                                                                                                                                              
tGCCGAGCAGTTGCTGCGCAATGCCGACTCGGCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCa                                                                                                                                               <  1:1176680/150‑1 (MQ=255)
tGCCGAGCAGTTGCTGCGCAATGCCGACTCGGCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCACAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCa                                                                                                                                               >  1:1192580/1‑150 (MQ=255)
    gAGCAGTTGCTGCGCAATGCCGACTCGGCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGAt                                                                                                                                           <  1:728774/150‑1 (MQ=255)
          ttGCTGCGCAATGCCGACTCGGCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTg                                                                                                                                     >  2:443203/1‑150 (MQ=255)
            gctgcGCAATGCCGACTCGGCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCg                                                                                                                                   >  2:1272347/1‑150 (MQ=255)
                    aTGCCGACTCGGCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCgg                                                                                                                                  >  1:1214780/1‑143 (MQ=255)
                    aTGCCGACTCGGCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCgg                                                                                                                                  <  2:1214780/143‑1 (MQ=255)
                        cgagccGGCATTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCa                                                                                                                       <  2:1035936/145‑1 (MQ=255)
                              ggCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAg                                                                                                                      >  1:899640/1‑145 (MQ=255)
                              ggCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAg                                                                                                                      <  2:899640/145‑1 (MQ=255)
                               gCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGTAGGATGAACTGCGGGTGTACTTCCAGCCGGTa                                                                                                                >  1:430192/1‑150 (MQ=255)
                               gCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGTAGGATGAACTGCGGGTGTACTTCCAGCCGGTa                                                                                                                >  1:430207/1‑150 (MQ=255)
                               gCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTa                                                                                                                <  1:991923/150‑1 (MQ=255)
                                 gTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTaca                                                                                                              >  1:267773/1‑150 (MQ=255)
                                     tACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGAc                                                                                                          >  1:307958/1‑150 (MQ=255)
                                          ggCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGc                                                                                                     <  1:1272347/150‑1 (MQ=255)
                                            ccAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGgt                                                                                                                                >  1:98776/1‑121 (MQ=255)
                                            ccAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGgt                                                                                                                                <  2:98776/121‑1 (MQ=255)
                                            ccAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCACAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGt                                                                                                   <  2:1192580/150‑1 (MQ=255)
                                             ctaaaGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGACCACGCCCAGCATCGGGTCGAGACCGCCGATGACTTGTGTCGGGCCCTTTATCATGATATACGGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTc                                                                                                  <  1:666151/148‑1 (MQ=255)
                                             cAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTac                                                                                                               <  2:1216659/137‑1 (MQ=255)
                                             cAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTac                                                                                                               >  1:1216659/1‑137 (MQ=255)
                                             cAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTc                                                                                                  >  1:11019/1‑150 (MQ=255)
                                             cAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTc                                                                                                  >  2:158447/1‑150 (MQ=255)
                                              aaaaGCAACGGTCGGGCCTGCTATGCACTGTACACCGAAGAGCTGACCACCCACGCCCAGCATCGGGGCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGGCa                                                                                                 >  2:339496/1‑150 (MQ=255)
                                                aaGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCaag                                                                                               >  2:666151/1‑148 (MQ=255)
                                                  gCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACTGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGcc                                                                                             <  1:595561/150‑1 (MQ=255)
                                                        ggcgggcCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAgg                                                                                       >  2:551334/1‑150 (MQ=255)
                                                         gcgggcCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGt                                                                                      >  2:338601/1‑150 (MQ=255)
                                                             gcCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCggg                                                                                  <  1:937858/150‑1 (MQ=255)
                                                              ccTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCgggg                                                                                 >  2:399646/1‑150 (MQ=255)
                                                              ccTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCgggg                                                                                 >  1:519870/1‑150 (MQ=255)
                                                               cTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGt                                                                                >  1:969485/1‑150 (MQ=255)
                                                                       cgcTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGc                                                                        <  2:11019/150‑1 (MQ=255)
                                                                        gcTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCCACATGTCGTGGTCGAGACGCt                                                                       <  2:1017570/150‑1 (MQ=255)
                                                                            tACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGgt                                                                                                                                <  2:578379/89‑1 (MQ=255)
                                                                            tACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGgt                                                                                                                                >  1:578379/1‑89 (MQ=255)
                                                                            tACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGgt                                                                                                                                <  2:578231/89‑1 (MQ=255)
                                                                            tACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGgt                                                                                                                                >  1:578231/1‑89 (MQ=255)
                                                                                      agCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGTGGTTACTTCCAGCCGGTACACGACCTGGGGTCAGGCCGACAGGTCGGGGGCGAGAGGCTGGTGCGCTGgcagc                                                         >  1:86068/1‑150 (MQ=255)
                                                                                        cTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCAc                                                       >  2:762286/1‑150 (MQ=255)
                                                                                           aCCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCAcccc                                                    >  2:1119649/1‑150 (MQ=255)
                                                                                                ccacgcccaGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGgcg                                               >  2:347819/1‑150 (MQ=255)
                                                                                                      ccaGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCt                                         <  1:443203/150‑1 (MQ=255)
                                                                                                       caGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTg                                        <  1:551334/150‑1 (MQ=255)
                                                                                                          cATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGTAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTa                                     <  2:430207/150‑1 (MQ=255)
                                                                                                          cATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGTAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTa                                     <  2:430192/150‑1 (MQ=255)
                                                                                                                 tCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCgg                              >  2:1047368/1‑150 (MQ=255)
                                                                                                                   gagaCCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGt                            <  2:519870/150‑1 (MQ=255)
                                                                                                                   gagaCCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGt                            >  2:956982/1‑150 (MQ=255)
                                                                                                                      aCCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGTGAg                         <  1:338601/150‑1 (MQ=255)
                                                                                                                       ccgccgCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGTGAGt                        <  1:134954/150‑1 (MQ=255)
                                                                                                                                   tGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGTGAGTTCATCCCGATTg            <  2:938342/150‑1 (MQ=255)
                                                                                                                                         gggCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACTAAACTGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGTGAGTTCATCCCGATTGCCGAAc      <  1:347819/150‑1 (MQ=255)
                                                                                                                                             ccTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGTGAGTTCATCCCGATTGCCGAACGCAc  <  2:307958/150‑1 (MQ=255)
                                                                                                                                             ccTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGTGAGTTCATCCCGATTGCCGAACGCAc  <  1:1047368/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TGCCGAGCAGTTGCTGCGCAATGCCGACTCGGCGTTGTACAAGGCCAAAAGCAACGGGCGGGCCTGCTATGCGCTGTACACCGAAGAGCTGACCGCCCACGCCCAGCATCGGGTCGAGACCGCCGCTGAATTGCGTCGGGCCCTGGAGCAGGATGAACTGCGGGTGTACTTCCAGCCGGTACACGACCTGGCGTCAGGCCGACAGGTCGGGGTCGAGACGCTGGTGCGCTGGCAGCACCCCAGGCGCGGTCTGGTACCGCCGGGTGAGTTCATCCCGATTGCCGAACGCAC  >  NC_002947/271134‑271424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑