Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,260,665 C→G 6.5% G714A (GGC→GCC)  PP_3734 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,260,6650CG 6.5% 190.9 / 3.4 61G714A (GGC→GCC) PP_3734ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (34/23);  new base G (2/2);  total (36/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

TCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAGGCGCGCCTGCTCGA  >  NC_002947/4260516‑4260809
                                                                                                                                                     |                                                                                                                                                
tCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCCGAATCGCCAGCAGTAGGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGc                                                                                                                                                  <  1:520553/150‑1 (MQ=255)
       tGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCcg                                                                                                                                           >  2:188489/1‑150 (MQ=255)
              aggcaccaggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTgg                                                                                                                                    <  2:775763/150‑1 (MQ=255)
               ggcaccaggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGc                                                                                                                                   >  1:491995/1‑150 (MQ=255)
                             aaCAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAgg                                                                                                                     >  2:303261/1‑150 (MQ=255)
                              aCAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGt                                                                                                                    >  1:492178/1‑150 (MQ=255)
                               cAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCTCCGCTGCGCCTGGCCCAGGGTCAGCAGGtt                                                                                                                   >  1:73/1‑150 (MQ=255)
                                           aagcGCCAGCAGTACGGTGAAGGCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGc                                                                                                       <  1:346103/147‑1 (MQ=255)
                                            aTCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCAAAGCGGGGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGcc                                                                                                      >  1:12309/1‑150 (MQ=255)
                                               gCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGATGTCGCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCcaca                                                                                                   >  1:622674/1‑150 (MQ=255)
                                                ccAGCAGTAGGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCacac                                                                                                  >  2:494196/1‑150 (MQ=255)
                                                ccAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCacac                                                                                                  >  1:1140039/1‑150 (MQ=255)
                                                 cagcagTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACAcc                                                                                                 >  2:1049246/1‑150 (MQ=255)
                                                   gcagTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCgg                                                                                               <  1:722340/150‑1 (MQ=255)
                                                       tACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCTCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGCCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCAc                                                                                           >  1:726367/1‑150 (MQ=255)
                                                            tGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCATTCGCATGAGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACCCCGGCCACGCCaa                                                                                      >  2:441154/1‑150 (MQ=255)
                                                              aaGCCGCGGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAgg                                                                                    <  2:219600/150‑1 (MQ=255)
                                                                  cGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTaa                                                                                >  2:615686/1‑150 (MQ=255)
                                                                   gCTGAGCATGAGCGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAg                                                                               >  2:318381/1‑150 (MQ=255)
                                                                         cATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGtt                                                                         <  2:492178/150‑1 (MQ=255)
                                                                          aTGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTg                                                                        >  1:692050/1‑150 (MQ=255)
                                                                           tGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTga                                                                       <  2:692050/150‑1 (MQ=255)
                                                                                 tCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTg                                                                 >  1:708870/1‑150 (MQ=255)
                                                                                 ccTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTg                                                                 >  2:255043/2‑150 (MQ=255)
                                                                                     ccccAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGt                                                             >  2:986442/1‑150 (MQ=255)
                                                                                        cAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTggc                                                          >  2:100520/1‑150 (MQ=255)
                                                                                          ggCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTCCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTggcgg                                                        <  1:494196/150‑1 (MQ=255)
                                                                                               aaCCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAg                                                   <  1:1049246/150‑1 (MQ=255)
                                                                                                accagaccagTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAgg                                                  <  1:318381/150‑1 (MQ=255)
                                                                                                    gaccagTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCg                                              <  2:708870/150‑1 (MQ=255)
                                                                                                     accagTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCgg                                             <  2:1140039/150‑1 (MQ=255)
                                                                                                     accagTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCgg                                             >  1:153611/1‑150 (MQ=255)
                                                                                                       cagTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGtt                                           <  1:188489/150‑1 (MQ=255)
                                                                                                              aTGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCCCGCCAAGGGTAAGGCCGTTGAGAGCTGCGGTGGCCGCAAAGGTGCGGTTGAAGACg                                    >  2:199993/1‑150 (MQ=255)
                                                                                                              aTGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTATGGCTGtt                                                                         >  2:1144977/1‑113 (MQ=255)
                                                                                                              aTGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTATGGCTGtt                                                                         >  2:1144888/1‑113 (MQ=255)
                                                                                                              aTGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTATGGCTGtt                                                                         <  1:1144977/113‑1 (MQ=255)
                                                                                                              aTGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTATGGCTGtt                                                                         <  1:1144888/113‑1 (MQ=255)
                                                                                                              aTGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCg                                                               >  1:129237/1‑123 (MQ=255)
                                                                                                              aTGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCg                                                               <  2:129237/123‑1 (MQ=255)
                                                                                                              aTGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACg                                    >  2:1251239/1‑150 (MQ=255)
                                                                                                                  gccgcACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCgg                                             <  2:250827/137‑1 (MQ=255)
                                                                                                                  gccgcACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCgg                                             >  1:250827/1‑137 (MQ=255)
                                                                                                                       aCCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGAc                           >  2:310769/1‑150 (MQ=255)
                                                                                                                         ccccAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCa                         >  1:625472/1‑150 (MQ=255)
                                                                                                                           ccAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTgag                                                                      >  2:826316/1‑103 (MQ=255)
                                                                                                                           ccAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTgag                                                                      <  1:826316/103‑1 (MQ=255)
                                                                                                                                  ccACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAg                >  2:858980/1‑150 (MQ=255)
                                                                                                                                   cacaGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAg                                                   >  1:63058/1‑114 (MQ=255)
                                                                                                                                   cacaGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAg                                                   <  2:63058/114‑1 (MQ=255)
                                                                                                                                      aGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTCCAGgcgc            <  2:961630/150‑1 (MQ=255)
                                                                                                                                        cGGTGCTAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAGgcgcgc          <  1:441154/150‑1 (MQ=255)
                                                                                                                                            gCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAGGCGCGCCTGc      <  1:100520/150‑1 (MQ=255)
                                                                                                                                              cAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAGGCGCGCCTGCTc    >  1:484828/1‑150 (MQ=255)
                                                                                                                                              cAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAGGCGCGCCTGCTc    <  1:303261/150‑1 (MQ=255)
                                                                                                                                              cAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGAAGGCGGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAGGCGCGCCTGCTc    <  2:622674/150‑1 (MQ=255)
                                                                                                                                               aaCTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAGGCGCGCCTGCTCg   >  2:1062347/1‑150 (MQ=255)
                                                                                                                                                ccTGGGCCAGGCGCGGCTGGGCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTc                  <  2:1227709/133‑1 (MQ=255)
                                                                                                                                                ccTGGGCCAGGCGCGGCTGGGCCAGGGTCAGCAGGTTGATCAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTc                  >  1:1227709/2‑134 (MQ=255)
                                                                                                                                                aCTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAGGCGCGCCTGCTCGa  >  2:1214695/1‑150 (MQ=255)
                                                                                                                                                  tGGGCCAGGCGCGGCTGGGCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGa                            >  2:413438/1‑122 (MQ=255)
                                                                                                                                                  tGGGCCAGGCGCGGCTGGGCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCGGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGa                            <  1:413438/122‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGCGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGACCAGCGTTTCAGGCGCGCCTGCTCGA  >  NC_002947/4260516‑4260809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑