| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,311,359 | T→A | 11.1% | F12Y (TTT→TAT) ‡ | PP_3784 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,311,359 | 0 | T | A | 11.1% | 150.9 / 10.8 | 54 | F12Y (TTT→TAT) ‡ | PP_3784 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (25/23); new base A (3/3); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01 | |||||||||||
ACACAACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAGTGAGC > NC_002947/4311211‑4311507 | acacAACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAttt > 1:26486/1‑150 (MQ=255) aaCCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGt > 2:550231/1‑150 (MQ=255) aaCCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTACCTCCGGGGGCAACGCATTTGCGt > 1:95086/1‑150 (MQ=255) ccGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTg > 1:862144/1‑150 (MQ=255) gTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGTATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCt < 1:67350/150‑1 (MQ=255) gTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCt > 2:67350/1‑150 (MQ=255) tCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGc > 1:1021755/1‑136 (MQ=255) tCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGc < 2:1021755/136‑1 (MQ=255) tCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCg > 2:570116/1‑150 (MQ=255) tCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGtcat > 2:1011033/1‑150 (MQ=255) gCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAAtt > 1:1124725/1‑150 (MQ=255) gCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAttt > 2:602872/1‑113 (MQ=255) gCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAttt < 1:602872/113‑1 (MQ=255) tttAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTg < 2:1213127/150‑1 (MQ=255) tttAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTg > 2:818869/1‑150 (MQ=255) aaCCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCa < 2:1044779/150‑1 (MQ=255) aaCCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCa < 2:862144/150‑1 (MQ=255) aaCCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAAATGCGTTGcc > 1:717524/1‑115 (MQ=255) aaCCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAAATGCGTTGcc < 2:717524/115‑1 (MQ=255) aCCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCAc > 1:147312/1‑150 (MQ=255) ccGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATtcatca > 1:515941/1‑142 (MQ=255) ccGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATtcatca < 2:515941/142‑1 (MQ=255) cGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACgt < 1:668653/150‑1 (MQ=255) cGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGt < 2:374623/121‑1 (MQ=255) cGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGt > 1:374623/1‑121 (MQ=255) gTAACGCTCTGGCACCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTACCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCt < 2:95086/150‑1 (MQ=255) aaCGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGa < 1:512315/150‑1 (MQ=255) gCCCCCTAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGTCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAg < 2:644201/150‑1 (MQ=255) cccGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGa < 1:550231/150‑1 (MQ=255) cccGAAGCGATTTCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGCCTCTCGTCAGCAATTCATCAGTGCCCCGTTGACGCCTGACACCTTCAGCgc > 2:119915/1‑149 (MQ=255) aTTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTc < 2:1124725/150‑1 (MQ=255) gcCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACACCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCa > 1:1221566/1‑150 (MQ=255) cccGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTgcc > 1:1260072/1‑150 (MQ=255) aaaGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGc > 1:3812/1‑150 (MQ=255) gATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACAtt > 2:418755/1‑106 (MQ=255) gATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACAtt < 1:418755/106‑1 (MQ=255) gATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCt < 2:336972/118‑1 (MQ=255) gATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAAc > 2:399213/1‑150 (MQ=255) gATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAAc < 2:1270894/150‑1 (MQ=255) gATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGACACTGCCt > 1:336972/1‑118 (MQ=255) tcatcaATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAg > 2:1138736/1‑150 (MQ=255) gcgaaCAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGCCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACtt < 1:1240882/147‑1 (MQ=255) aaCAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACCCCTGACAAATTCAGCCACTGCTTGTCGCAATCATTGGCGTGATGCTGCAACGAACAGGCACCACTTGGc < 2:147312/150‑1 (MQ=255) aGCATTCCCTCCTGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGtg > 2:776972/1‑150 (MQ=255) aGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGtg > 2:723830/1‑150 (MQ=255) gCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGtgc < 1:818869/150‑1 (MQ=255) cATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTc < 2:1057101/102‑1 (MQ=255) cATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTc > 1:1057101/1‑102 (MQ=255) ttCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGtgctgc > 1:123051/1‑150 (MQ=255) ccTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTc < 1:1011033/150‑1 (MQ=255) cTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCt > 1:469947/1‑150 (MQ=255) gaagggCAACGCAAATGCGTTGCCCCTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATcc < 2:968775/109‑1 (MQ=255) gaagggCAACGCAAATGCGTTGCCCCTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATcc > 1:968775/4‑112 (MQ=255) gCAACGCAAATGCGTTGCCCTTCGCCGGCTCTCGtcatc < 2:224197/39‑1 (MQ=21) gCAACGCAAATGCGTTGCCCTTCGCCGGCTCTCGtcatc > 1:224197/1‑39 (MQ=21) cGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAGt < 1:399213/150‑1 (MQ=255) aTTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAGTGAg < 1:723830/150‑1 (MQ=255) tttGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAGTGAGc < 1:1138736/150‑1 (MQ=255) | ACACAACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAGTGAGC > NC_002947/4311211‑4311507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |