Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,375,490 T→G 17.3% R30R (CGA→CGC)  PP_5637 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,375,4900TG17.3% 101.6 / 16.6 51R30R (CGA→CGC) PP_5637hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base G (6/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑GGCACCAATACTGTATAAAGAT  >  NC_002947/4375352‑4375639
                                                                                                                                          |                                                                                                                                                      
atatAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGGTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAgg                                                                                                                                             <  2:858771/150‑1 (MQ=255)
  ataGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGTCTGGGTCGAGTCGCGACCCaggcctg                                                                                                                                           <  2:44452/150‑6 (MQ=255)
     gCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGc                                                                                                                                        <  2:1061463/150‑1 (MQ=255)
            ttagACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATg                                                                                                                                 <  1:908656/147‑1 (MQ=255)
                         aCCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTgg                                                                                                                    >  1:551280/1‑150 (MQ=255)
                           cccGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGAc                                                                                                                  <  1:546451/150‑1 (MQ=255)
                                 gatgttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCcgc                                                                                                            <  1:823455/150‑1 (MQ=255)
                                    gttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCcgcgcg                                                                                                         >  1:808730/1‑150 (MQ=255)
                                             gAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTg                                                                                                >  1:1028656/1‑150 (MQ=255)
                                                     ccATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTcc                                                                                        >  2:631026/1‑150 (MQ=255)
                                                        tGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTa                                                                                     <  2:828854/150‑1 (MQ=255)
                                                          ccGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAgg                                                                                   <  2:551280/150‑1 (MQ=255)
                                                           cGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGCGGGGCTGGACTTTAAGTAggg                                                                                  >  2:780603/1‑149 (MQ=255)
                                                               gACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTc                                                                              >  1:1185310/1‑150 (MQ=255)
                                                                 ccTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTg                                                                            >  1:9881/1‑150 (MQ=255)
                                                                  cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGa                                                                           >  2:631178/1‑150 (MQ=255)
                                                                        aTCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCaa                                                                     >  2:113201/1‑150 (MQ=255)
                                                                         tCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAg                                                                    <  2:808730/150‑1 (MQ=255)
                                                                           gTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGc                                                                  <  1:629153/150‑1 (MQ=255)
                                                                            tGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGcc                                                                 >  1:96037/1‑150 (MQ=255)
                                                                              cAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCAc                                                               <  2:620157/150‑1 (MQ=255)
                                                                                gCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGc                                                             >  1:70017/1‑150 (MQ=255)
                                                                                   gggggATAATGTCGGGCCCAGGCGTAGGGTTAAACAGCCAGGCCTGGGTCGCGGCGCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCAt                                                          >  2:718725/1‑150 (MQ=255)
                                                                                    ggggATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGa                                                                                              <  1:318705/113‑1 (MQ=255)
                                                                                    ggggATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGa                                                                                              >  2:318705/1‑113 (MQ=255)
                                                                                       gATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCATGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTc                                                      <  1:780603/150‑1 (MQ=255)
                                                                                              tCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAAt                                                                                                                        >  1:1267059/1‑77 (MQ=255)
                                                                                              tCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAAt                                                                                                                        <  2:1267059/77‑1 (MQ=255)
                                                                                               cGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTc                                              <  1:1183779/150‑1 (MQ=255)
                                                                                                gggCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTACGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCg                                             >  2:1216829/1‑150 (MQ=255)
                                                                                                  gCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGaa                                           >  1:626142/1‑150 (MQ=255)
                                                                                                   cccAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAAc                                          <  2:754169/150‑1 (MQ=255)
                                                                                                      aGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACgag                                       <  2:70017/150‑1 (MQ=255)
                                                                                                               gTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTa                              <  1:113201/150‑1 (MQ=255)
                                                                                                                    acacCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGc                         <  2:1028656/150‑1 (MQ=255)
                                                                                                                    acacCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGc                         >  2:456934/1‑150 (MQ=255)
                                                                                                                    acacCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGc                         <  1:631026/150‑1 (MQ=255)
                                                                                                                      acCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑gg                      >  2:219108/1‑150 (MQ=255)
                                                                                                                        ccAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑GGCa                    >  2:1246590/1‑150 (MQ=255)
                                                                                                                         agcctcTGGGTCGAGTCGCGACCCAGGGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGcc                                                            >  2:155157/6‑110 (MQ=255)
                                                                                                                         agcctcTGGGTCGAGTCGCGACCCAGGGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGcc                                                            <  1:155157/105‑1 (MQ=255)
                                                                                                                         agcctcTGGGTCGAGTCGCGACCCAGGGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑GGCAc                   >  1:210887/6‑150 (MQ=255)
                                                                                                                            ctcTGGGTCGAGTCGCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCa                                                                <  2:97934/101‑1 (MQ=255)
                                                                                                                            ctcTGGGTCGAGTCGCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCa                                                                >  1:97934/3‑103 (MQ=255)
                                                                                                                            ctcTGGGTCGAGTCGCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑GGCACCaa                >  2:1285606/3‑150 (MQ=255)
                                                                                                                            ctcTGGGTCGAGTCGCGACCCAGAGGCTAGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAGGGAAGGAC‑TGCGGGCACCaa                >  2:614568/3‑150 (MQ=255)
                                                                                                                                  gTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑GGCACCAATACTGt          >  2:1033303/1‑150 (MQ=255)
                                                                                                                                    cgcgACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCTGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑GGCACCAATACTGtat        >  2:149110/1‑150 (MQ=255)
                                                                                                                                     gcgACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAg                                                                    >  2:1011359/1‑90 (MQ=255)
                                                                                                                                     gcgACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAg                                                                    <  1:1011359/90‑1 (MQ=255)
                                                                                                                                     gcgACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑GGCACCAATACTGtata       <  1:117251/150‑1 (MQ=255)
                                                                                                                                          tCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑GGCACCAATACTGTATAAAGAt  >  1:763729/1‑150 (MQ=255)
                                                                                                                                          |                                                                                                                                                      
ATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGC‑GGCACCAATACTGTATAAAGAT  >  NC_002947/4375352‑4375639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑