Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,394,578 T→A 6.3% V146V (GTA→GTT)  PP_3869 ← sheath protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,394,5780TA 6.3% 186.7 / 4.3 63V146V (GTA→GTT) PP_3869sheath protein
Reads supporting (aligned to +/- strand):  ref base T (27/32);  new base A (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

GAGGCCAGCAGGAGTCAACTCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTGATGGTCACCTTGCCGGCCGCCTTGGTA  >  NC_002947/4394431‑4394725
                                                                                                                                                    |                                                                                                                                                   
gAGGCCAGCAGGAGTCACCTCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTAc                                                                                                                                                   <  2:1071956/150‑1 (MQ=255)
 aGGCCAGCAGGAGTCAACTCGCCGTTCCTATTACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTAcc                                                                                                                                                  >  1:997742/1‑150 (MQ=255)
   gCCAGCAGGAGTCAACTCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTAccgc                                                                                                                                                >  1:356903/1‑150 (MQ=255)
   gCCAGCAGGAGTCAACTCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTAccgc                                                                                                                                                >  1:356888/1‑150 (MQ=255)
           gAGTCAACTCGCTGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCa                                                                                                                                        >  2:996375/1‑150 (MQ=255)
           gAGTCAACTCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCa                                                                                                                                        <  2:1133944/150‑1 (MQ=255)
            aGTCAACTCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGGTACGCCAAGGCCGGCGGCGTTTACCGCAGCCGCCAg                                                                                                                                       >  1:12197/1‑150 (MQ=255)
                aaCTCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCa                                                                                                                                   >  2:854951/1‑150 (MQ=255)
                 aCTCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAg                                                                                                                                  >  2:119383/1‑150 (MQ=255)
                   tCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTg                                                                                                                                <  1:1188773/150‑1 (MQ=255)
                    cGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTTGTTAAGCGACGCGG‑CGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTgg                                                                                                                               <  2:997742/150‑1 (MQ=255)
                     gCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTAc                                                                                                                                                   <  1:1070018/129‑1 (MQ=255)
                     gCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTAc                                                                                                                                                   >  2:1070018/1‑129 (MQ=255)
                       cGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCa                                                                                                                            <  1:854951/150‑1 (MQ=255)
                        gTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCAc                                                                                                                           >  2:900828/1‑150 (MQ=255)
                        gTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCAc                                                                                                                           <  1:900828/150‑1 (MQ=255)
                               cGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTgcc                                                                                                                    <  2:1221595/150‑1 (MQ=255)
                                 aCCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTgccgc                                                                                                                  >  1:836911/1‑150 (MQ=255)
                                   ccTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGccgccg                                                                                                                >  2:992102/1‑150 (MQ=255)
                                   ccTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGGTAAacgccgccgg                                                                                                                                          >  2:764852/1‑123 (MQ=255)
                                   ccTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGGTAAacgccgccgg                                                                                                                                          <  1:764852/124‑2 (MQ=255)
                                      gACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTgg                                                                                                                               >  2:19161/1‑132 (MQ=255)
                                      gACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTgg                                                                                                                               <  1:19161/132‑1 (MQ=255)
                                       aCGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGTGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGgc                                                                                                            <  1:320336/150‑1 (MQ=255)
                                         ggTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGgcgc                                                                                                          >  2:396428/1‑150 (MQ=255)
                                                       tGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTa                                                                                            <  2:665541/150‑1 (MQ=255)
                                                         gATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAgc                                                                                          >  1:515973/1‑150 (MQ=255)
                                                             tCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTCgcgcgc                                                                                      >  1:77014/1‑150 (MQ=255)
                                                                       cTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTgg                                                                             <  1:996375/150‑1 (MQ=255)
                                                                       cTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTgg                                                                             <  1:119383/150‑1 (MQ=255)
                                                                               ggACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGAt                                                                     <  2:709573/150‑1 (MQ=255)
                                                                                gACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAgc                                                                                          >  2:797816/1‑128 (MQ=255)
                                                                                gACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAgc                                                                                          <  1:797816/128‑1 (MQ=255)
                                                                                  ccAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGACAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTa                                                                  <  1:281715/150‑1 (MQ=255)
                                                                                   cAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTAc                                                                 <  2:515973/150‑1 (MQ=255)
                                                                                     gCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAg                                                               >  1:497614/1‑150 (MQ=255)
                                                                                      cGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAgg                                                              <  2:745663/150‑1 (MQ=255)
                                                                                          aGGTCAGGCCCCCTCCCACCGGGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCATTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGa                                                          <  2:145283/150‑1 (MQ=255)
                                                                                              cAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTg                                                      <  2:836911/150‑1 (MQ=255)
                                                                                               aGGGCCACTTCACCCGTGGCTGCAGTCGCCGCTACGCCAAGGCCGGCGGCGTTTACCGCCGCCGACAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTgg                                                     <  2:683576/150‑1 (MQ=255)
                                                                                               aGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTgg                                                     <  2:583412/150‑1 (MQ=255)
                                                                                                        tCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCCTGGCGCCGATGTACAGGTTGACCTGGCCACCCTcc                                            <  1:103484/150‑1 (MQ=255)
                                                                                                          aCCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGt                                          >  1:663027/1‑150 (MQ=255)
                                                                                                            ccGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTgg                                        <  2:356903/150‑1 (MQ=255)
                                                                                                            ccGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTgg                                        <  2:356888/150‑1 (MQ=255)
                                                                                                            ccGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTgg                                        <  1:140691/150‑1 (MQ=255)
                                                                                                            ccGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCCCCGTgg                                        >  2:576059/1‑150 (MQ=255)
                                                                                                                  cTGCAGTCACCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGc                                  >  2:1133557/1‑150 (MQ=255)
                                                                                                                    gCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGAGATCGCCGCCGATGTACAGGTTGACCTGGCCCCCCTCCGACTCGGTGCCg                                <  2:689713/150‑1 (MQ=255)
                                                                                                                    gCAGTCACCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCg                                <  1:1133557/150‑1 (MQ=255)
                                                                                                                      aGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGt                              >  2:860952/1‑150 (MQ=255)
                                                                                                                          gCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTGATg                          <  1:860952/150‑1 (MQ=255)
                                                                                                                          gCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCCAGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTTATg                          <  1:91541/150‑1 (MQ=255)
                                                                                                                           ccGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTGATgg                         >  2:363184/1‑150 (MQ=255)
                                                                                                                                  gCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGTTGCCGGTGATGGTCACCtt                  >  2:937112/1‑150 (MQ=255)
                                                                                                                                  gCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTGATGGTCACCtc                  >  2:538604/1‑149 (MQ=255)
                                                                                                                                   ccAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTGATGGTCACCTTg                 >  1:1100389/1‑150 (MQ=255)
                                                                                                                                       tggcggcggcggTAAACGCCGCCGGCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTAc                                                                                                <  2:245425/64‑1 (MQ=21)
                                                                                                                                       tggcggcggcggTAAACGCCGCCGGCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTAc                                                                                                >  1:245425/4‑67 (MQ=21)
                                                                                                                                       ggCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGt                              >  2:1083163/1‑133 (MQ=255)
                                                                                                                                       ggCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGt                              <  1:1083163/133‑1 (MQ=255)
                                                                                                                                               gcgTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTGATGGTCACCTTGCCGGCCGCCTTg     <  2:313507/150‑1 (MQ=255)
                                                                                                                                                 gTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTGATGGTCACCTTGCCGGCCGCCTTGGt   >  2:241421/1‑150 (MQ=255)
                                                                                                                                                  tttACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTGATGGTCACCTTGCCGGCCGCCTTGGTa  >  2:640133/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGGCCAGCAGGAGTCAACTCGCCGTTGCTGCGACCCTGACGGTTCAGTTCCAGCTGGATGTCGTT‑GCCGCTGAGGCCGGACCAGCGGCAGGTCAGGGCCACTTCACCCGTGGCTGCAGTCGCCGATACGCCAAGGCCGGCGGCGTTTACCGCCGCCGCCAGCGCAGTGGCCACCGCTGCCGCCGAGGCGCCACTGGCTACCGTAGCGCGCACGCGCTGGCCGCCGATGTACAGGTTGACCTGGCCACCCTCCGTGGCGGTGCCGGTGATGGTCACCTTGCCGGCCGCCTTGGTA  >  NC_002947/4394431‑4394725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑