Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,483,958 T→C 14.2% L82S (TTG→TCG)  PP_3976 → isochorismatase family hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,483,9580TC14.2% 76.7 / 4.8 35L82S (TTG→TCG) PP_3976isochorismatase family hydrolase
Reads supporting (aligned to +/- strand):  ref base T (15/15);  new base C (2/3);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

GGCAGACGCTGAAGAACAACACGGTAGGTTTGGCCAAAGCAGCCAGGATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCTGCAACACCACCTTCGCGCTGTCCGCCATGCACGATGC  >  NC_002947/4483814‑4484102
                                                                                                                                                |                                                                                                                                                
ggCAGACGCTGAAGAACAACACGGTAGGTTTGGCCAAAGCAGCCAGGATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTg                                                                                                                                             >  2:682320/1‑150 (MQ=255)
 gCAGACGCTGAAGAACAACACGGTAGGTTTGGCCAAAGCAGCCAGGATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGa                                                                                                                                            >  1:450234/1‑150 (MQ=255)
                 aacaCGGTAGGTTTGGCCAAAGCAGCCAGGATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAAc                                                                                                                            <  2:946681/150‑1 (MQ=255)
                       gTAGGTTTGGCCAAAGCAGCCAGGATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCCAGCGCGCCCTAGCGCACCTCCATGAACTCCtag                                                                                                                      <  1:997569/150‑3 (MQ=255)
                                   aaaGCAGCCAGGATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAg                                                                                                          >  1:150289/1‑150 (MQ=255)
                                   aaaGCAGCCAGGATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAg                                                                                                          >  1:288704/1‑150 (MQ=255)
                                             ggATCTTTAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCAt                                                                                                                                <  2:745549/118‑1 (MQ=255)
                                             ggATCTTTAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCAt                                                                                                                                >  1:745549/1‑118 (MQ=255)
                                             ggATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTAGAAGTATACCCTGATGCGCCATTGCTTGAGCGCACCTCCCTGAACTCCTGGGACGACCAGAAGGTTCGAGAcg                                                                                                >  1:988034/1‑150 (MQ=255)
                                              gATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGAcgc                                                                                               >  1:122426/1‑150 (MQ=255)
                                               aTCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGAcgcg                                                                                              <  1:867670/150‑1 (MQ=255)
                                                    cAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTgaa                                                                                         <  2:455823/150‑1 (MQ=255)
                                                    cAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTgaa                                                                                         <  2:888570/150‑1 (MQ=255)
                                                       tgtgCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTCCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTgaagaa                                                                                      <  1:1032350/150‑1 (MQ=255)
                                                         tgCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGaa                                                                                                                             <  1:268729/109‑1 (MQ=255)
                                                         tgCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGaa                                                                                                                             >  2:268729/1‑109 (MQ=255)
                                                            cGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATg                                                                                 <  1:813664/150‑1 (MQ=255)
                                                                              tCGAAACCGAGAGTTTCTCCGGGCATCCCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCg                                                               >  1:570654/1‑150 (MQ=255)
                                                                                   aCCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGACCTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCg                                                          <  2:450234/150‑1 (MQ=255)
                                                                                           tttCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATCGCTTGAGCGCACCTCCATGAACTCCTGGgacgac                                                                                                                >  1:607500/1‑88 (MQ=255)
                                                                                           tttCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATCGCTTGAGCGCACCTCCATGAACTCCTGGgacgac                                                                                                                <  2:607500/88‑1 (MQ=255)
                                                                                              ctcCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCg                                                          <  1:888693/139‑1 (MQ=255)
                                                                                              ctcCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCg                                                          >  2:888693/1‑139 (MQ=255)
                                                                                                    gCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAAGAAGATCATCGTTTCGGGCTTGTGGACAGAGGt                                         <  2:988034/150‑1 (MQ=255)
                                                                                                          ctaccCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCTGCaa                                   >  2:465372/1‑150 (MQ=255)
                                                                                                                               cTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCt                                       >  2:1247483/1‑125 (MQ=255)
                                                                                                                               cTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCt                                       <  1:1247483/125‑1 (MQ=255)
                                                                                                                                   ccTGATGCGCCATCGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCgag                                                                                                   >  2:26566/1‑61 (MQ=255)
                                                                                                                                   ccTGATGCGCCATCGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCgag                                                                                                   <  1:26566/61‑1 (MQ=255)
                                                                                                                                    cTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCTGCAACACCACCTTc                         <  2:234270/134‑1 (MQ=255)
                                                                                                                                    cTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCTGCAACACCACCTTc                         >  1:234270/1‑134 (MQ=255)
                                                                                                                                     tGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCTGCAAcaccac                             >  2:1142769/1‑129 (MQ=255)
                                                                                                                                     tGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCTGCAAcaccac                             <  1:1142769/129‑1 (MQ=255)
                                                                                                                                         gcgcCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCTGCAACACCACCTTCGCGCTGTCCGCCATGCACGAt    <  1:682320/150‑1 (MQ=255)
                                                                                                                                           gcCATTGCTTGAGCGCACCTCCATGAACTCCTAGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAAAAAGATCATCGTTTCGGGCTTGTGGACAAAGGTCTACAACACCACCTTCGAGCTGTCCGCCATGCACGATGc  >  2:423950/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GGCAGACGCTGAAGAACAACACGGTAGGTTTGGCCAAAGCAGCCAGGATCTTCAATGTGCCGACCGTCTTCACCACGGTCGAAACCGAGAGTTTCTCCGGGCATACCTACCCCGAGCTGCTCGCGGTCTTCCCTGATGCGCCATTGCTTGAGCGCACCTCCATGAACTCCTGGGACGACCAGAAGGTTCGAGACGCGCTGAAGAAGAATGGTCGGAACAAGATCATCGTTTCGGGCTTGTGGACAGAGGTCTGCAACACCACCTTCGCGCTGTCCGCCATGCACGATGC  >  NC_002947/4483814‑4484102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑