Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,501,386 T→C 7.5% noncoding (62/88 nt) PP_t60 ← tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,501,3860TC 7.5% 152.4 / 2.9 53noncoding (62/88 nt)PP_t60tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (26/23);  new base C (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

GCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTCGCGGGTTGCAGGAGCTCTGATAGACTC  >  NC_002947/4501238‑4501533
                                                                                                                                                    |                                                                                                                                                   
gCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATTGCGGAAGCGTAGAGATTCGAACTCTa                                                                                                                                                    <  2:640409/150‑1 (MQ=255)
        cATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGtt                                                                                                                                            >  2:74393/1‑150 (MQ=255)
        cATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGtt                                                                                                                                            <  1:751276/150‑1 (MQ=255)
                 gATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGa                                                                                                                                   >  2:764629/1‑150 (MQ=255)
                 gATGCGGAAACGATCAGAGAATTGCGAAAACGACCCGGATGACAAAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGa                                                                                                                                   <  2:864364/150‑1 (MQ=255)
                      ggAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGtt                                                                                                                              <  1:709929/150‑1 (MQ=255)
                        aaaCGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGGAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGt                                                                                                                                             <  1:16435/133‑1 (MQ=255)
                        aaaCGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGGAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGt                                                                                                                                             >  2:16435/1‑133 (MQ=255)
                                agagAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCg                                                                                                                    >  1:601043/1‑150 (MQ=255)
                                 gagaATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGAc                                                                                                                                  <  2:756017/135‑1 (MQ=255)
                                 gagaATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGAc                                                                                                                                  >  1:756017/1‑135 (MQ=255)
                                          ggAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTaaa                                                                                                          <  2:354807/150‑1 (MQ=255)
                                           gAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAAc                                                                                                         >  1:141674/1‑150 (MQ=255)
                                                gACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTc                                                                                                    >  2:785400/1‑150 (MQ=255)
                                                      gATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCAc                                                                                              >  1:726508/1‑150 (MQ=255)
                                                            aGAAGGGAAAAAACTAGAGCCTCAGAAACGCACAAAGAAATCCATGCCAAGAGCGATGAAGAGGGGGGTAGTTTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTcc                                                                                        >  1:23959/1‑150 (MQ=255)
                                                                  gAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCg                                                                                  >  2:256738/1‑150 (MQ=255)
                                                                  gAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCg                                                                                  >  1:784895/1‑150 (MQ=255)
                                                                          tAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAAc                                                                                                         >  2:1051596/1‑119 (MQ=255)
                                                                          tAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAAc                                                                                                         <  1:1051596/119‑1 (MQ=255)
                                                                          tAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCgg                                                                          >  1:749787/1‑150 (MQ=255)
                                                                          tAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCgg                                                                          >  1:785403/1‑150 (MQ=255)
                                                                              gCCTCAGAAACGCAAAAAGCCCTGAATATGCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGgccgc                                                                      >  1:874981/1‑150 (MQ=255)
                                                                              gCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGcc                                                                                                               <  1:514153/109‑1 (MQ=255)
                                                                              gCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGcc                                                                                                               >  2:514153/1‑109 (MQ=255)
                                                                                cTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCa                                                                    >  1:808879/1‑150 (MQ=255)
                                                                                  cAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCata                                                                  <  2:784895/150‑1 (MQ=255)
                                                                                   aGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCATGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCataa                                                                 <  2:829808/150‑1 (MQ=255)
                                                                                   aGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCataa                                                                 <  2:726508/150‑1 (MQ=255)
                                                                                     aaaCGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAAACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCataata                                                               >  1:1207814/1‑150 (MQ=255)
                                                                                       aCGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATAcc                                                             <  2:749787/150‑1 (MQ=255)
                                                                                           aaaaaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTaa                                                         <  2:23959/150‑1 (MQ=255)
                                                                                             aaaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCCAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTaaa                                                                                                          >  1:470833/1‑99 (MQ=255)
                                                                                             aaaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCCAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTaaa                                                                                                          <  2:470833/99‑1 (MQ=255)
                                                                                              aaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGa                                                      <  1:74393/150‑1 (MQ=255)
                                                                                                    tGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACg                                                <  1:785400/150‑1 (MQ=255)
                                                                                                    tGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACg                                                >  1:47713/1‑150 (MQ=255)
                                                                                                         aatCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACCCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTc                                           <  2:874981/150‑1 (MQ=255)
                                                                                                           tCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGGACTCTAGGATAGTTGCCCATCGACGGTTTACAAGACCGTTGCCTTAAACCACTCAGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCaa                                         >  2:1041077/1‑150 (MQ=255)
                                                                                                                gCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCataa                                                                 >  1:774207/1‑121 (MQ=255)
                                                                                                                gCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCataa                                                                 <  2:774207/121‑1 (MQ=255)
                                                                                                                 cTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAActctc                                   <  2:141674/150‑1 (MQ=255)
                                                                                                                 cTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCCAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAActctc                                   >  1:333910/1‑150 (MQ=255)
                                                                                                                  tttGAATATGGCGGAAGCGTAGAGATTCGAACTCCAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAActctct                                  <  2:333910/150‑1 (MQ=255)
                                                                                                                                    gTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCAGGATTCCGGCTAGCATTGCGTCCGCAATAATACCTTAATGAAACACGCTGTCAAACTCTCCTGGCCGCGGGTGGCAGGa                >  2:15836/1‑150 (MQ=255)
                                                                                                                                    gTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTcg                            >  2:1176955/1‑138 (MQ=255)
                                                                                                                                    gTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTcg                            <  1:1176955/138‑1 (MQ=255)
                                                                                                                                    gTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTCGCGGGTTGCAg                  >  2:112678/1‑148 (MQ=255)
                                                                                                                                    gTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTCGCGGGTTGCAg                  <  1:112678/148‑1 (MQ=255)
                                                                                                                                    gTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTCGCGGGTTGCAGGa                >  2:159389/1‑150 (MQ=255)
                                                                                                                                      agagATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTCGCGGGTTGCAGGAGc              <  1:159389/150‑1 (MQ=255)
                                                                                                                                        agaTTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTCGCGGGTTGCAGGAGctc            <  1:764629/150‑1 (MQ=255)
                                                                                                                                          atgcGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTCGCGGGTTGCAGGAGCTCTg          >  1:45345/4‑150 (MQ=255)
                                                                                                                                                  tctAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTCGCGGGTTGCAGGAGCTCTGATAGACTc  >  1:771741/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACGCTGTCAAACTCTCTGTGTCGCGGGTTGCAGGAGCTCTGATAGACTC  >  NC_002947/4501238‑4501533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑