| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,528,397 | T→A | 13.9% | intergenic (+28/‑48) | purB → / → PP_4017 | adenylosuccinate lyase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,528,397 | 0 | T | A | 13.9% | 95.2 / 7.8 | 36 | intergenic (+28/‑48) | purB/PP_4017 | adenylosuccinate lyase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (18/13); new base A (2/3); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
GCGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTGGTA > NC_002947/4528253‑4528540 | gcgcTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCATGGCCg > 1:218011/1‑150 (MQ=255) gctgctGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCggg > 2:614682/1‑150 (MQ=255) ctgACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCGtt < 1:285770/150‑1 (MQ=255) gACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCGttgt < 2:1006369/150‑3 (MQ=255) cTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGaaaa > 2:614116/1‑150 (MQ=255) gCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGttt > 2:623951/1‑150 (MQ=255) gCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCAACTCCAGTGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGttt > 1:719775/1‑150 (MQ=255) cAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGttttt > 2:106344/1‑150 (MQ=255) ccAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGt > 2:974627/1‑150 (MQ=255) gCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGaa < 2:192827/150‑1 (MQ=255) aaGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCt > 2:22375/1‑150 (MQ=255) aaGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTAACAAAATAGCGCTGGCCGGGCGTTTTTATTCCCGTACGAAAATAACGTATTTACAGCAGGTTGCACGTGGAGCCt > 1:403888/1‑150 (MQ=255) gACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTTCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTcc > 1:180699/1‑150 (MQ=255) gACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTcc > 1:180581/1‑150 (MQ=255) cGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTAAGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCa < 2:1012381/150‑1 (MQ=255) gCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAg < 1:974627/150‑1 (MQ=255) cGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTcggcgg < 1:614682/150‑1 (MQ=255) aGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTAGGCCcg > 1:1214929/1‑150 (MQ=255) gCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCcgc < 1:106344/150‑1 (MQ=255) cAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCcgagca > 1:146285/1‑146 (MQ=255) ggCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAAtt < 2:666247/150‑1 (MQ=255) cTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGacta < 1:22375/150‑1 (MQ=255) tAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGactac < 1:614116/150‑1 (MQ=255) gCTGAGCGTCGCGTACAACGCGCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATGGGTTCAACATGAATCCTGATACTCCAGTGCAGCTGCTCGGCGGCATCTCGGCCCGGGAATGCATGCGCGACTATTgt < 2:403888/150‑2 (MQ=255) gTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGACGGCAtctc > 1:699561/1‑115 (MQ=255) gTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGACGGCAtctc < 2:699561/115‑1 (MQ=255) gcgTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTgg > 2:774618/1‑140 (MQ=255) gcgTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTgg < 1:774618/140‑1 (MQ=255) gcgTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAAAACTCCAGTTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGCACTCACCCGCAAATAGTAGCAGAAGAAGc > 1:404020/1‑150 (MQ=255) aaaCGCCCGGCCAGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTcggc > 2:1263655/2‑99 (MQ=255) aaaCGCCCGGCCAGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTcggc < 1:1263655/98‑1 (MQ=255) aaCGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCgctgc > 1:277238/1‑150 (MQ=255) aaCGCCCGGCCAGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCgctgc > 1:563637/1‑150 (MQ=255) cGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGACTTCATGCGCGACTACTGGCAGAAGAAGCCGctgctg < 1:1066230/150‑1 (MQ=255) cGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTAGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGctgctg < 2:1214929/150‑1 (MQ=255) ccGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTGGTa > 1:188496/1‑150 (MQ=255) | GCGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTGGTA > NC_002947/4528253‑4528540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |