Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,635,810 C→A 64.3% intergenic (‑9/+55) uvrY ← / ← PP_4100 BarA/UvrY two‑componenent system response regulator/Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,635,8100CA64.3% 39.9 / 35.3 47intergenic (‑9/+55)uvrY/PP_4100BarA/UvrY two‑componenent system response regulator/Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (10/7);  new base A (17/13);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCACTATTTGCCGGGGCTTGGCGTCGTTG  >  NC_002947/4635665‑4635943
                                                                                                                                                 |                                                                                                                                     
aGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACCTCCCTCTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGt                                                                                                                                   >  1:404024/1‑150 (MQ=255)
                           tCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCt                                                                                                        >  1:405701/1‑150 (MQ=255)
                                ggAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGGGCGTACGAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCGCGTCAGCAGGGCGATTGGATGCGCCCACCTTGACa                                                                                                   >  2:126699/1‑150 (MQ=255)
                                        ccTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAAttttt                                                                                           >  1:166535/1‑150 (MQ=255)
                                            acccaccACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTca                                                                                                                                 >  1:650460/1‑108 (MQ=255)
                                            acccaccACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTca                                                                                                                                 <  2:650460/108‑1 (MQ=255)
                                                accaccTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTgg                                                                                   >  2:980558/1‑150 (MQ=255)
                                                  caccTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAAc                                                                                                    >  1:824810/1‑131 (MQ=255)
                                                  caccTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAAc                                                                                                    <  2:824810/131‑1 (MQ=255)
                                                          ggCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCa                                                                         >  2:1162769/1‑150 (MQ=255)
                                                           gCCGGCGATGTCGCCCAACATCCGTGTCATTCCGCTTTGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCGCACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCAt                                                                        <  2:166535/150‑1 (MQ=255)
                                                              gTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGc                                                                     <  2:405701/150‑1 (MQ=255)
                                                                  aTGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGcttcc                                                                 >  1:17682/1‑150 (MQ=255)
                                                                    gTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAAtttt                                                                                            <  1:347039/121‑1 (MQ=255)
                                                                    gTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAAtttt                                                                                            >  2:347039/1‑121 (MQ=255)
                                                                       ggCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAAc                                                                            >  2:751265/1‑134 (MQ=255)
                                                                       ggCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAAc                                                                            <  1:751265/134‑1 (MQ=255)
                                                                       ggCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCAt                                                                        <  1:432596/138‑1 (MQ=255)
                                                                       ggCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCAt                                                                        >  2:432596/1‑138 (MQ=255)
                                                                       ggCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAg                                                            >  1:1176144/1‑150 (MQ=255)
                                                                              aTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAAc                                                                            >  2:895262/1‑127 (MQ=255)
                                                                              aTCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAAc                                                                            <  1:895262/127‑1 (MQ=255)
                                                                                   tgtAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACg                                                >  2:949580/1‑150 (MQ=255)
                                                                                     tAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGAc                                                     <  2:55236/143‑1 (MQ=255)
                                                                                     tAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGAc                                                     >  1:55236/1‑143 (MQ=255)
                                                                                       aTACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCg                                            >  1:566523/1‑150 (MQ=255)
                                                                                                  tACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCgg                                 >  1:1058078/1‑150 (MQ=255)
                                                                                                    ccAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGcc                               >  1:1158790/1‑150 (MQ=255)
                                                                                                     cAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAACCGACTGACGTTCGACAGTAAGCGGCCg                              <  1:126699/150‑1 (MQ=255)
                                                                                                     cAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGcttccttc                                                              >  2:1080495/1‑118 (MQ=255)
                                                                                                     cAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGcttccttc                                                              <  1:1080495/118‑1 (MQ=255)
                                                                                                            tGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGAt                                                                                                                  <  2:987987/59‑1 (MQ=255)
                                                                                                            tGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGAt                                                                                                                  >  1:987987/1‑59 (MQ=255)
                                                                                                            tGATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCACTAtt                       >  2:683367/1‑150 (MQ=255)
                                                                                                             gATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTgg                                                                                   <  2:613985/89‑1 (MQ=255)
                                                                                                             gATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTgg                                                                                   >  1:613985/1‑89 (MQ=255)
                                                                                                             gATCATCGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCACTAttt                      >  1:570676/1‑150 (MQ=255)
                                                                                                                 atcTACTACTACGACCCGAACAAACCACACACATCGTCAGCAGGGCGGTTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCATAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCACTATTTGCCg                  <  2:566523/150‑1 (MQ=255)
                                                                                                                 atcGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTc                                                                                          >  2:256937/1‑78 (MQ=255)
                                                                                                                 atcGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTc                                                                                          <  1:256937/78‑1 (MQ=255)
                                                                                                                 atcGACCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCACTATTTGCCg                  <  2:1058078/150‑1 (MQ=255)
                                                                                                                     aCCACTAAGACCCTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCACTATTTGCCGGGGc              <  2:17682/150‑1 (MQ=255)
                                                                                                                        aCTAAGACCCTAATCCAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCATGATTTCCCcggccgtt           <  2:404024/150‑6 (MQ=255)
                                                                                                                           aaGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAAttttt                                                                                           <  2:591766/67‑1 (MQ=255)
                                                                                                                           aaGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAAttttt                                                                                           >  1:591766/1‑67 (MQ=255)
                                                                                                                                 cTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCACTATTTGCCGGGGCTTGGCGTCGTTg  >  1:478785/1‑150 (MQ=255)
                                                                                                                                 cTAATCAAGCACACACATCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCACTATTTGCCGGGGCTTGGCGTCGTTg  <  2:1158790/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                     
AGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTCACGGTGGGCCTAACGCATTGCTTCCTTCAGAAACGACTGACGTTCGACAGTAAGCGGCCGCACTATTTGCCGGGGCTTGGCGTCGTTG  >  NC_002947/4635665‑4635943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑