Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,708,858 T→A 8.9% H104L (CAC→CTC)  PP_4165 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,708,8580TA 8.9% 124.3 / 5.5 45H104L (CAC→CTC) PP_4165hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/18);  new base A (2/2);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

CCAGGGTGCGCCCGGCCGGTGTGATACGGTCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCTGGGCCAGGTGCTGCACCTGA  >  NC_002947/4708709‑4709007
                                                                                                                                                     |                                                                                                                                                     
ccAGGGTGCGCCCGGCCGGTGTGATACGGTCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGt                                                                                                                                                       >  1:614181/1‑150 (MQ=255)
                  gtgtGATACGGTCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCg                                                                                                                                     >  1:1045035/1‑150 (MQ=255)
                   tgtgATACGGTCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGa                                                                                                                                    >  1:1026736/1‑150 (MQ=255)
                      gATACGGTCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATg                                                                                                                                 <  2:614181/150‑1 (MQ=255)
                       aTACGGTCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATgg                                                                                                                                <  2:1026736/150‑1 (MQ=255)
                       aTACGGTCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATgg                                                                                                                                >  2:1053773/1‑150 (MQ=255)
                             tCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAAt                                                                                                                                  >  2:59281/1‑142 (MQ=255)
                             tCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAAt                                                                                                                                  <  1:59281/142‑1 (MQ=255)
                             tCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACg                                                                                                                          >  2:1199103/1‑150 (MQ=255)
                                     aCCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGTCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGAc                                                                                                                  >  1:226702/1‑150 (MQ=255)
                                     aCCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCATCGCCCCAGAc                                                                                                                  <  2:1244734/150‑1 (MQ=255)
                                                   cTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTTGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCctgct                                                                                                    >  1:1140516/1‑150 (MQ=255)
                                                      tgCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTgcg                                                                                                 >  2:797523/1‑150 (MQ=255)
                                                         gcagcaGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGAGCAATGCGACGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGCCCGGCTGATCCTGCTGCGCCa                                                                                              >  1:498131/1‑150 (MQ=255)
                                                               gcCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGAt                                                                                        <  2:502985/150‑1 (MQ=255)
                                                                 cATTGATCAGCGCGGGTGAGCACCGGATAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGATGCGCCAGGTGATGc                                                                                      >  2:609697/1‑150 (MQ=255)
                                                                   ttGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGcaa                                                                                    >  1:159419/1‑150 (MQ=255)
                                                                         aGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTc                                                                              >  2:665370/1‑150 (MQ=255)
                                                                          gcgcGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCa                                                                             >  1:1253360/1‑150 (MQ=255)
                                                                           cgcgGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCAt                                                                            <  1:1163165/150‑1 (MQ=255)
                                                                               ggTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAAAAGGCAAACCAGCGAATAGCGAACGCTCGAGACCGGCGGATCCTGCTGCGCCCGGTGATGCAACAATTCATCGAc                                                                        >  2:1042164/1‑150 (MQ=255)
                                                                                              gTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGtt                                                         <  2:958041/150‑1 (MQ=255)
                                                                                               tCGAGGATCAGCAGCCCCCGGTAGTACGCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCGTCGACCAGGCTTTGCCAGttt                                                        >  2:660136/1‑150 (MQ=255)
                                                                                               tCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGttt                                                        >  1:810773/1‑150 (MQ=255)
                                                                                                   gCATCACCAGCCCCCGGTAGTACTCGGGGCAGTGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGt                                                    >  2:1239889/1‑150 (MQ=255)
                                                                                                      tCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGAGCAACACGCCACCCAGCGAATGGCCTACGCCCCAGACCGGCTGATCctgc                                                                                                     <  1:495273/98‑1 (MQ=255)
                                                                                                      tCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGAGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCctgc                                                                                                     <  1:1138147/98‑1 (MQ=255)
                                                                                                      tCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGAGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCctgc                                                                                                     >  2:495273/1‑98 (MQ=255)
                                                                                                      tCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGAGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCctgc                                                                                                     >  2:1138147/1‑98 (MQ=255)
                                                                                                       cACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCAc                                                >  1:1061419/1‑150 (MQ=255)
                                                                                                        aCCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACg                                               >  1:255920/1‑150 (MQ=255)
                                                                                                         ccAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACgg                                              >  1:970056/1‑150 (MQ=255)
                                                                                                               cccGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAAc                                        <  2:255920/150‑1 (MQ=255)
                                                                                                                   gtagtaCTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCgg                                    <  1:1199103/150‑1 (MQ=255)
                                                                                                                       taCTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGGCCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCa                                >  1:464854/1‑150 (MQ=255)
                                                                                                                            ggggCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATgcgc                           <  2:1253360/150‑1 (MQ=255)
                                                                                                                            ggggCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATgcgc                           >  1:1107875/1‑150 (MQ=255)
                                                                                                                              ggCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCACCAGGTGATGCAACAATGGATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCAt                         <  1:797523/150‑1 (MQ=255)
                                                                                                                               gCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATg                        <  2:1129280/150‑1 (MQ=255)
                                                                                                                                 aGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCt                      <  2:1061419/150‑1 (MQ=255)
                                                                                                                                   cgcAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCTgg                    <  1:1053773/150‑1 (MQ=255)
                                                                                                                                   cgcAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCTgg                    <  2:1045035/150‑1 (MQ=255)
                                                                                                                                    gcAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCTggg                   <  2:970056/150‑1 (MQ=255)
                                                                                                                                        tGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCTGGGCCAg               >  2:1073899/1‑150 (MQ=255)
                                                                                                                                           cgcgTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCTGGGCCAGGtg            <  2:159419/150‑1 (MQ=255)
                                                                                                                                            gcgTGCAGGTGCAACACGCCACCCAGCGAATGGCCACCGCCCCAGACCGGCTGATCCTGCTGCGCCAGTTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCTGGGCCAGGtgc           <  1:609697/150‑1 (MQ=255)
                                                                                                                                                     tGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCTGGGCCAGGTGCTGCACCTGa  <  1:1046410/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGGGTGCGCCCGGCCGGTGTGATACGGTCGATGAAACCAAAGCGCTTGGCTGTGCGCAGCAGCCATTGATCAGCGCGGGTGAGCACCGGAGAGTCGAGCATCACCAGCCCCCGGTAGTACTCGGGGCAGCGCAATGCCGCGTGCAGGTGCAACACGCCACCCAGCGAATGGCCAACGCCCCAGACCGGCTGATCCTGCTGCGCCAGGTGATGCAACAATTCATCGACCAGGCTTTGCCAGTTTTCGTCCACGGGAAAACGCGGGTCATGCGCATGCTGGGCCAGGTGCTGCACCTGA  >  NC_002947/4708709‑4709007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑