Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,744,784 G→A 5.8% A29A (GCC→GCT)  PP_4198 ← OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,744,7840GA 5.8% 199.9 / 2.6 69A29A (GCC→GCT) PP_4198OmpA family protein
Reads supporting (aligned to +/- strand):  ref base G (33/32);  new base A (2/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

GCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTG  >  NC_002947/4744636‑4744929
                                                                                                                                                    |                                                                                                                                                 
gCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCgg                                                                                                                                                  >  1:413309/1‑150 (MQ=255)
                gCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggat                                                                                                                                  <  2:666974/150‑4 (MQ=255)
                   gATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgc                                                                                                                               >  2:489302/1‑150 (MQ=255)
                   gATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGAACTGCTGCGCTGACTGgc                                                                                                                               <  1:43795/150‑1 (MQ=255)
                    aTGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgcg                                                                                                                              >  2:563230/1‑150 (MQ=255)
                             gACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGc                                                                                                                     >  2:765939/1‑150 (MQ=255)
                                aGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCaaa                                                                                                                  <  1:507994/150‑1 (MQ=255)
                                aGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCACGGCACTGCTGCGCTGACTGGCGCAACCTTGCaaa                                                                                                                  >  1:7215/1‑150 (MQ=255)
                                 ggCGCTCGGCGCGAGCCAGCTACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAAc                                                                                                                 <  1:518469/150‑1 (MQ=255)
                                  gcgcTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAAcc                                                                                                                >  2:1133421/1‑150 (MQ=255)
                                      tCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGcc                                                                                                            >  1:635365/1‑150 (MQ=255)
                                          cgcgAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCa                                                                                                        <  2:413309/150‑1 (MQ=255)
                                             gAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAgcg                                                                                                     >  1:1229063/1‑150 (MQ=255)
                                                  aGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGc                                                                                                >  1:188715/1‑150 (MQ=255)
                                                        tCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAAc                                                                                                                 >  1:1091575/1‑127 (MQ=255)
                                                        tCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAAc                                                                                                                 <  2:1091575/127‑1 (MQ=255)
                                                        tCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGcc                                                                                          <  1:120766/150‑1 (MQ=255)
                                                          aCCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAg                                                                                        >  2:650872/1‑150 (MQ=255)
                                                            ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCCTATTGGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTg                                                                                      >  1:873682/1‑150 (MQ=255)
                                                            ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTg                                                                                      >  1:858536/1‑150 (MQ=255)
                                                                  gAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCa                                                                                >  1:830284/1‑150 (MQ=255)
                                                                   aaTCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCaa                                                                               >  1:489014/1‑150 (MQ=255)
                                                                     tcacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAg                                                                             >  2:1071416/1‑150 (MQ=255)
                                                                      cacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAgg                                                                            <  2:635365/150‑1 (MQ=255)
                                                                       acgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCCGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGc                                                                           <  2:858536/150‑1 (MQ=255)
                                                                        cgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCgg                                                                                                                                                  <  2:1237682/78‑1 (MQ=255)
                                                                        cgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCgg                                                                                                                                                  >  1:1237682/1‑78 (MQ=255)
                                                                         gtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGt                                                                         >  1:168224/1‑150 (MQ=255)
                                                                          tcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTa                                                                        <  1:563230/150‑1 (MQ=255)
                                                                              gcggcgCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTacgac                                                                    >  2:539463/1‑150 (MQ=255)
                                                                              gcggcgCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTacgac                                                                    >  2:711592/1‑150 (MQ=255)
                                                                                 gcgcgcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGa                                                                 <  1:650872/150‑1 (MQ=255)
                                                                                 gcgcgcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGa                                                                 <  1:309929/150‑1 (MQ=255)
                                                                                     gcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCa                                                              <  2:681728/149‑1 (MQ=255)
                                                                                     gcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCa                                                              >  1:681728/1‑149 (MQ=255)
                                                                                     gcTGCGCAGAACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGAGAGGCATGGTTGCGCTGCCCGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCAt                                                             <  1:1055083/150‑1 (MQ=255)
                                                                                       tGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                           >  1:939083/1‑150 (MQ=255)
                                                                                          gcagcaCATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTg                                                        >  1:121961/1‑150 (MQ=255)
                                                                                           cagcaCATCGGAATCATCTTTGTCCCCCTGGAACGCAACGATGGACTAATACAGCGCGGTACTTCCGCGTTAATTTTCTTATCCTTGTAAACCTGCCTTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGc                                                       <  2:1109078/150‑1 (MQ=255)
                                                                                             gcaCATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCAc                                                     >  2:831151/1‑150 (MQ=255)
                                                                                                caTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                  >  2:1051766/1‑150 (MQ=255)
                                                                                                caTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                  <  1:489302/150‑1 (MQ=255)
                                                                                                caTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTCGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                  <  1:711592/150‑1 (MQ=255)
                                                                                                 aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTcc                                                 >  1:451072/1‑150 (MQ=255)
                                                                                                  tCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCa                                                >  2:1025970/1‑150 (MQ=255)
                                                                                                     gAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                             <  2:939083/150‑1 (MQ=255)
                                                                                                      aaTCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                  <  1:442620/144‑1 (MQ=255)
                                                                                                      aaTCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                  >  2:442620/1‑144 (MQ=255)
                                                                                                         catcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgctt                                         <  2:489014/150‑1 (MQ=255)
                                                                                                               tGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgct                                          >  2:638985/1‑143 (MQ=255)
                                                                                                               tGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgct                                          <  1:638985/143‑1 (MQ=255)
                                                                                                               tGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCaa                                   <  1:765939/150‑1 (MQ=255)
                                                                                                                  cccccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCACCAATTCCTTGTGCAAgcg                                >  2:1197379/1‑150 (MQ=255)
                                                                                                                   ccccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAgcgc                               >  2:565250/1‑150 (MQ=255)
                                                                                                                    cccTGGAAGTCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                              <  1:429225/150‑1 (MQ=255)
                                                                                                                    cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCACCGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                              <  2:1175246/150‑1 (MQ=255)
                                                                                                                    cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                              <  1:819083/150‑1 (MQ=255)
                                                                                                                    cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                              >  2:695699/1‑150 (MQ=255)
                                                                                                                    cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                              >  2:819083/1‑150 (MQ=255)
                                                                                                                     ccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgcttgc                                       <  2:603090/140‑1 (MQ=255)
                                                                                                                     ccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgcttgc                                       >  1:603090/1‑140 (MQ=255)
                                                                                                                         gAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGAcc                         <  2:121961/150‑1 (MQ=255)
                                                                                                                          aaGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGAccc                        <  1:831151/150‑1 (MQ=255)
                                                                                                                                 cGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTg                 <  2:451072/150‑1 (MQ=255)
                                                                                                                                 cGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTg                 <  2:168224/150‑1 (MQ=255)
                                                                                                                                  ggTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTGCGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTgc                <  2:830284/150‑1 (MQ=255)
                                                                                                                                       ccTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAc           >  2:507617/1‑150 (MQ=255)
                                                                                                                                          cATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTg        <  2:1229063/150‑1 (MQ=255)
                                                                                                                                            gtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGAccc                        >  2:531002/3‑132 (MQ=255)
                                                                                                                                            gtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGAccc                        <  1:531002/130‑1 (MQ=255)
                                                                                                                                               aGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAgct   >  2:179871/1‑150 (MQ=255)
                                                                                                                                                gcgcGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAgctg  <  1:1133421/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTG  >  NC_002947/4744636‑4744929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑