Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,744,792 T→C 6.7% S27G (AGC→GGC)  PP_4198 ← OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,744,7920TC 6.7% 206.7 / 2.6 70S27G (AGC→GGC) PP_4198OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (33/32);  new base C (2/3);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTGCTCTTC  >  NC_002947/4744652‑4744935
                                                                                                                                            |                                                                                                                                               
gCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTggat                                                                                                                                        <  2:666974/150‑4 (MQ=255)
   gATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgc                                                                                                                                     >  2:489302/1‑150 (MQ=255)
   gATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGAACTGCTGCGCTGACTGgc                                                                                                                                     <  1:43795/150‑1 (MQ=255)
    aTGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGgcg                                                                                                                                    >  2:563230/1‑150 (MQ=255)
             gACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGc                                                                                                                           >  2:765939/1‑150 (MQ=255)
                aGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCaaa                                                                                                                        <  1:507994/150‑1 (MQ=255)
                aGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCACGGCACTGCTGCGCTGACTGGCGCAACCTTGCaaa                                                                                                                        >  1:7215/1‑150 (MQ=255)
                 ggCGCTCGGCGCGAGCCAGCTACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAAc                                                                                                                       <  1:518469/150‑1 (MQ=255)
                  gcgcTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAAcc                                                                                                                      >  2:1133421/1‑150 (MQ=255)
                      tCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGcc                                                                                                                  >  1:635365/1‑150 (MQ=255)
                          cgcgAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCa                                                                                                              <  2:413309/150‑1 (MQ=255)
                             gAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAgcg                                                                                                           >  1:1229063/1‑150 (MQ=255)
                                  aGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGc                                                                                                      >  1:188715/1‑150 (MQ=255)
                                        tCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAAc                                                                                                                       >  1:1091575/1‑127 (MQ=255)
                                        tCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAAc                                                                                                                       <  2:1091575/127‑1 (MQ=255)
                                        tCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGcc                                                                                                <  1:120766/150‑1 (MQ=255)
                                          aCCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAg                                                                                              >  2:650872/1‑150 (MQ=255)
                                            ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCCTATTGGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTg                                                                                            >  1:873682/1‑150 (MQ=255)
                                            ccgcccgAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTg                                                                                            >  1:858536/1‑150 (MQ=255)
                                                  gAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCa                                                                                      >  1:830284/1‑150 (MQ=255)
                                                   aaTCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCaa                                                                                     >  1:489014/1‑150 (MQ=255)
                                                     tcacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAg                                                                                   >  2:1071416/1‑150 (MQ=255)
                                                      cacgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAgg                                                                                  <  2:635365/150‑1 (MQ=255)
                                                       acgtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCCGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGc                                                                                 <  2:858536/150‑1 (MQ=255)
                                                         gtcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGt                                                                               >  1:168224/1‑150 (MQ=255)
                                                          tcacgCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTa                                                                              <  1:563230/150‑1 (MQ=255)
                                                              gcggcgCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTacgac                                                                          >  2:711592/1‑150 (MQ=255)
                                                              gcggcgCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTacgac                                                                          >  2:539463/1‑150 (MQ=255)
                                                                 gcgcgcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGa                                                                       <  1:309929/150‑1 (MQ=255)
                                                                 gcgcgcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGa                                                                       <  1:650872/150‑1 (MQ=255)
                                                                     gcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCa                                                                    <  2:681728/149‑1 (MQ=255)
                                                                     gcTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCa                                                                    >  1:681728/1‑149 (MQ=255)
                                                                     gcTGCGCAGAACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGAGAGGCATGGTTGCGCTGCCCGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCAt                                                                   <  1:1055083/150‑1 (MQ=255)
                                                                       tGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATgg                                                                 >  1:939083/1‑150 (MQ=255)
                                                                          gcagcaCATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTg                                                              >  1:121961/1‑150 (MQ=255)
                                                                           cagcaCATCGGAATCATCTTTGTCCCCCTGGAACGCAACGATGGACTAATACAGCGCGGTACTTCCGCGTTAATTTTCTTATCCTTGTAAACCTGCCTTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGc                                                             <  2:1109078/150‑1 (MQ=255)
                                                                             gcaCATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCAc                                                           >  2:831151/1‑150 (MQ=255)
                                                                                caTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                        >  2:1051766/1‑150 (MQ=255)
                                                                                caTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                        <  1:489302/150‑1 (MQ=255)
                                                                                caTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTCGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                        <  1:711592/150‑1 (MQ=255)
                                                                                 aTCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTcc                                                       >  1:451072/1‑150 (MQ=255)
                                                                                  tCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCa                                                      >  2:1025970/1‑150 (MQ=255)
                                                                                     gAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGtt                                                   <  2:939083/150‑1 (MQ=255)
                                                                                      aaTCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                        <  1:442620/144‑1 (MQ=255)
                                                                                      aaTCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTc                                                        >  2:442620/1‑144 (MQ=255)
                                                                                         catcTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgctt                                               <  2:489014/150‑1 (MQ=255)
                                                                                               tGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgct                                                <  1:638985/143‑1 (MQ=255)
                                                                                               tGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgct                                                >  2:638985/1‑143 (MQ=255)
                                                                                               tGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCaa                                         <  1:765939/150‑1 (MQ=255)
                                                                                                  cccccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCACCAATTCCTTGTGCAAgcg                                      >  2:1197379/1‑150 (MQ=255)
                                                                                                   ccccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAgcgc                                     >  2:565250/1‑150 (MQ=255)
                                                                                                    cccTGGAAGTCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                    <  1:429225/150‑1 (MQ=255)
                                                                                                    cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCACCGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                    <  2:1175246/150‑1 (MQ=255)
                                                                                                    cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                    <  1:819083/150‑1 (MQ=255)
                                                                                                    cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                    >  2:819083/1‑150 (MQ=255)
                                                                                                    cccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCt                                    >  2:695699/1‑150 (MQ=255)
                                                                                                     ccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgcttgc                                             <  2:603090/140‑1 (MQ=255)
                                                                                                     ccTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGttgcttgc                                             >  1:603090/1‑140 (MQ=255)
                                                                                                         gAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGAcc                               <  2:121961/150‑1 (MQ=255)
                                                                                                          aaGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGAccc                              <  1:831151/150‑1 (MQ=255)
                                                                                                                 cGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTg                       <  2:168224/150‑1 (MQ=255)
                                                                                                                 cGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTg                       <  2:451072/150‑1 (MQ=255)
                                                                                                                  ggTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTGCGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTgc                      <  2:830284/150‑1 (MQ=255)
                                                                                                                       ccTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCAc                 >  2:507617/1‑150 (MQ=255)
                                                                                                                          cATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTg              <  2:1229063/150‑1 (MQ=255)
                                                                                                                            gtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGAccc                              <  1:531002/130‑1 (MQ=255)
                                                                                                                            gtcAGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGAccc                              >  2:531002/3‑132 (MQ=255)
                                                                                                                               aGCGCAGCAGTGCCGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAgct         >  2:179871/1‑150 (MQ=255)
                                                                                                                                gcgcGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAgctg        <  1:1133421/150‑1 (MQ=255)
                                                                                                                                      cACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTgctcttc  <  1:390491/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                               
GCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGACTGGCGCAACCTTGCAAACCTGCCGTCAGCGCCAGCAATGCCAGTGCAGCCAAAGGCGTACGACGGATCATGGCTGCACCTCCAGTTGCTTGCGCAAGCGCTTGACCCGCGACTGCAGCACCTGCAGCTGCTCTTC  >  NC_002947/4744652‑4744935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑