Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,848,653 A→T 8.2% intergenic (+13/‑93) PP_4263 → / → hemN cytochrome biogenesis protein/oxygen‑independent coproporphyrinogen III dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,848,6530AT 8.2% 143.6 / 2.9 48intergenic (+13/‑93)PP_4263/hemNcytochrome biogenesis protein/oxygen‑independent coproporphyrinogen III dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (22/22);  new base T (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGATCCGCCGCTACGATCTCGCCG  >  NC_002947/4848506‑4848800
                                                                                                                                                   |                                                                                                                                                   
aCCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTTGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAagc                                                                                                                                                   <  1:888811/150‑1 (MQ=255)
 ccGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCTAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAagca                                                                                                                                                  >  1:352835/1‑150 (MQ=255)
     gctggctgCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCTTTGCAGccc                                                                                                                                              <  1:1196802/150‑1 (MQ=255)
             ccGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCGGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGccc                                                                                                                                      >  1:235264/1‑150 (MQ=255)
              cGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCt                                                                                                                                     <  2:571505/150‑1 (MQ=255)
               gAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCtt                                                                                                                                    >  2:1007545/1‑150 (MQ=255)
                 aCGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGa                                                                                                                                  <  2:312318/150‑1 (MQ=255)
                 aCGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGa                                                                                                                                  <  1:226275/150‑1 (MQ=255)
                        aaCGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAAt                                                                                                                           >  2:143522/1‑150 (MQ=255)
                        aaCGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAAt                                                                                                                           <  1:143522/150‑1 (MQ=255)
                         aCGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATc                                                                                                                          >  2:764118/1‑150 (MQ=255)
                                    cggcgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTc                                                                                                               <  1:1007545/150‑1 (MQ=255)
                                     ggcgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTcc                                                                                                              <  1:220076/150‑1 (MQ=255)
                                     ggcgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTcc                                                                                                              <  1:764118/150‑1 (MQ=255)
                                       cgACGCAGAGTGCGAGAGGAAGGCGATGGGCTGGTGATGCTATTCGGTATCTGGGCTCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTa                                                                                                            <  2:235264/150‑1 (MQ=255)
                                        gACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCTCAATGCCCTTGATACAAATCAACACAGTTTCCTAc                                                                                                           <  1:303235/150‑1 (MQ=255)
                                               cGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACATACGGc                                                                                                    >  2:73523/1‑150 (MQ=255)
                                                   cGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATa                                                                                                <  2:155501/150‑1 (MQ=255)
                                                               ggTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGa                                                                                                                                  <  2:356092/104‑1 (MQ=255)
                                                               ggTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGa                                                                                                                                  >  1:356092/1‑104 (MQ=255)
                                                                       ggTGATGCTATTCGGTATCTGGCCCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTgctgct                                                                            <  1:493378/150‑1 (MQ=255)
                                                                                    ggTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTa                                                                                                            >  1:171878/1‑105 (MQ=255)
                                                                                    ggTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTa                                                                                                            <  2:171878/105‑1 (MQ=255)
                                                                                          tGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTAccc                                                           <  2:90771/148‑1 (MQ=255)
                                                                                          tGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTAccc                                                           >  1:90771/1‑148 (MQ=255)
                                                                                          tGGACCATGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTc                                                         >  1:930017/1‑150 (MQ=255)
                                                                                           ggACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCa                                                        >  2:1113557/1‑150 (MQ=255)
                                                                                               ccTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCt                                                    >  1:1200687/1‑150 (MQ=255)
                                                                                               ccTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCt                                                    >  2:1142464/1‑150 (MQ=255)
                                                                                                 tGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTc                                                                                                               >  1:1040946/1‑89 (MQ=255)
                                                                                                 tGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTc                                                                                                               <  2:1040946/89‑1 (MQ=255)
                                                                                                           acacCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTcc                                                                      <  2:250171/120‑1 (MQ=255)
                                                                                                           acacCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTcc                                                                      >  1:250171/1‑120 (MQ=255)
                                                                                                             acCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGtt                                      >  1:273014/1‑150 (MQ=255)
                                                                                                             acCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCAATGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGtt                                      >  1:1008611/1‑150 (MQ=255)
                                                                                                               cAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTgg                                    <  1:1142464/150‑1 (MQ=255)
                                                                                                                aGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACCGACGGCCATAGACTCCGGTGCAATGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTggg                                   <  2:1008611/150‑1 (MQ=255)
                                                                                                                   aCTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATa                                <  2:930017/150‑1 (MQ=255)
                                                                                                                       gCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGa                                                                                                                                  >  2:106187/1‑48 (MQ=255)
                                                                                                                       gCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGa                                                                                                                                  <  1:106187/48‑1 (MQ=255)
                                                                                                                        cTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGAc                           <  1:73523/150‑1 (MQ=255)
                                                                                                                         tGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACTCAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCgg                                                                    <  2:1250826/108‑1 (MQ=255)
                                                                                                                         tGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACTCAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCgg                                                                    >  1:1250826/1‑108 (MQ=255)
                                                                                                                              ggCCATTGACGGGGGCTGCAAACCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATACACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGCCGACCTCCGTTGGGATACCGACCTGATc                     >  1:215576/1‑150 (MQ=255)
                                                                                                                                  aTTGACGGGGGCTGCAAAGGAGCCCCAATGCCCTTGATACAAATCAACACAGGTTCCTACAGACGGCCATAGAATCCGGTGCACTGCTGCCCTTTCCGGGGACTACCCTCATTCTCGACGACCTCCCTTGGGATTCCGACCTGATccgcc                 >  2:944636/1‑150 (MQ=255)
                                                                                                                                     cattggggCTGCTTTGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGa                       >  1:451944/5‑141 (MQ=255)
                                                                                                                                     cattggggCTGCTTTGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGa                       <  2:451944/137‑1 (MQ=255)
                                                                                                                                      attggggCTGCTTTGCAGCCCCCATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGATCCGCCGCTa             >  1:449432/4‑150 (MQ=255)
                                                                                                                                              tGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGATCCGCCGCTACGATCTCg     >  2:242342/1‑150 (MQ=255)
                                                                                                                                              tGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGATCCGCCGCTACGATCTCg     >  1:236971/1‑150 (MQ=255)
                                                                                                                                                 aaaGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGATCCGCCGCTACGATCTCGCCg  >  2:889121/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGATCCGCCGCTACGATCTCGCCG  >  NC_002947/4848506‑4848800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑