| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,015,773 | C→A | 8.5% | A5S (GCT→TCT) | PP_5679 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,015,773 | 0 | C | A | 8.5% | 169.5 / 2.8 | 57 | A5S (GCT→TCT) | PP_5679 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (28/24); new base A (3/2); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01 | |||||||||||
GACGAGGATTCCGTTGGCATACTTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTT > NC_002947/5015624‑5015922 | gacgaGGATTCCGTTGGCATACTTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGc < 2:227420/150‑1 (MQ=255)gacgaGGATTCCGTTGGCATACTTGGCCTCATTAGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGc > 2:435966/1‑150 (MQ=255) ttCCGTTGGCATACTTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCgg > 1:170381/1‑150 (MQ=255) tCCGTTGGCATACTTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGt < 1:1059057/150‑1 (MQ=255) gCATACTTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCa > 1:523711/1‑136 (MQ=255) gCATACTTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCa < 2:523711/136‑1 (MQ=255) gCATACTTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTa < 1:786529/150‑1 (MQ=255) aTTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGa < 2:199545/150‑1 (MQ=255) tttGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAg < 2:170381/150‑1 (MQ=255) ggTTGTACATGCCCGTGCGGATACTCGCCCGTAGCCGCTTGCAGAGTGACTGCTGGGGACGAGGCTTCTCGCCAGCCCTGGCCGTATATGTAGCCATTGCTGCTTTGGTCGGTGCCAGAGCGGTCATCCTGGCGCTCTCGTGTGAGTCCa < 2:351593/150‑1 (MQ=255) gTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCAc > 2:1247825/1‑150 (MQ=255) gTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCCTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCAc > 2:928468/1‑150 (MQ=255) ttGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGt < 1:630960/122‑1 (MQ=255) ttGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGt > 2:630960/1‑122 (MQ=255) tGAACTGGCCCGTGCGGTTACGCACCTGTAGCCGCTTGCAGAGTGGCTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTccggta < 1:685475/150‑5 (MQ=255) gAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATc > 1:1144623/1‑124 (MQ=255) gAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATc < 2:1144623/124‑1 (MQ=255) tGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCAGGCTGGGTCATCCTAGCGCTCTCGTTTGAGTGCACgatgaga > 1:976744/1‑150 (MQ=255) gCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACgatgagatg > 2:688921/1‑150 (MQ=255) ccGTGCGGATCCTCCCCCGTAGCCCCTTGCACATCGACTGCTGGGCACTAAGCTTTTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGaa < 2:976744/150‑1 (MQ=255) ccGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGCATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGaa > 2:1257583/1‑150 (MQ=255) cGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAAt < 1:435966/150‑1 (MQ=255) gTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATa > 1:1100933/1‑150 (MQ=255) ggATACTCACCCCTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGcc > 1:29363/1‑150 (MQ=255) aCCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGCTCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCgg > 1:561524/1‑150 (MQ=255) aCCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCa > 2:1001557/1‑100 (MQ=255) aCCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCa < 1:1001557/100‑1 (MQ=255) gCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACgat < 2:497740/118‑1 (MQ=255) gCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACgat > 1:497740/1‑118 (MQ=255) ttGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGtc < 2:446350/140‑1 (MQ=255) ttGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGtc > 1:446350/1‑140 (MQ=255) agTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGc > 1:630619/1‑127 (MQ=255) agTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGc < 2:630619/127‑1 (MQ=255) gACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCCTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACt < 1:928468/150‑1 (MQ=255) tgGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGACAGCt < 2:1035781/67‑1 (MQ=255) tgGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGACAGCt > 1:1035781/1‑67 (MQ=255) tgGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGACAGCt > 1:1036222/1‑67 (MQ=255) tgGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGACAGCt < 2:1036222/67‑1 (MQ=255) aaGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGa < 1:688921/150‑1 (MQ=255) ttCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATaa > 2:966054/1‑100 (MQ=255) ttCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATaa < 1:966054/100‑1 (MQ=255) gCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGAccc > 1:1229347/1‑150 (MQ=255) cccTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCg > 2:334799/1‑150 (MQ=255) cTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCg < 1:590753/125‑1 (MQ=255) cTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCg > 2:590753/1‑125 (MQ=255) cTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGaa > 1:680807/1‑150 (MQ=255) tGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGaaa > 2:723448/1‑150 (MQ=255) ataGGTAGCCACTGCTTCCTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTc > 2:259539/1‑150 (MQ=255) ggTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCtt < 2:29363/150‑1 (MQ=255) gTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTtg < 2:1229347/150‑1 (MQ=255) gTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTtg > 2:144975/1‑150 (MQ=255) ccACTGCTTCTTTGCTCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGCGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGaa < 1:1226797/150‑1 (MQ=255) ccACTGCTTCCTTGATCGGCGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGaa < 1:259539/150‑1 (MQ=255) cACTGCTTCTTTGATCGGAGCCAGCTCGGTCCTCCTCGTGATCTCGTATGCGTTCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTGTCTGACGCGAAAGTACGTCCTTGTGAAc > 1:543416/1‑150 (MQ=255) aCTGCTTCTTTGATCGGAGACAGCTCTGTCAGCCGAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACt > 1:670484/1‑150 (MQ=255) gCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACtgat < 2:1100933/150‑1 (MQ=255) tttGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAg > 1:1131074/1‑150 (MQ=255) aTCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTc > 2:614087/1‑150 (MQ=255) gAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTgg > 2:953808/1‑150 (MQ=255) ccAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCtt < 2:1131074/150‑1 (MQ=255) | GACGAGGATTCCGTTGGCATACTTGGCCTCATTTGGGTTGAACTGGCCCGTGCGGATACTCACCCGTAGCCGCTTGCAGAGTGACTGCTGGGCACGAAGCTTCTCGGCAGCCCTGGACGTATAGGTAGCCACTGCTTCTTTGATCGGAGCCAGCTCGGTCATCCTAGCGCTCTCGTTTGAGTCCACGATGAGATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTT > NC_002947/5015624‑5015922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |