Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 335,799 G→C 11.3% noncoding (24/89 nt) PP_mr04 → C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947335,7990GC11.3% 156.6 / 10.5 53noncoding (24/89 nt)PP_mr04C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base C (3/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

TTCGGAAGTATCCTACGCGCGCCGCCGAAGTAACGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCCATGAAAAAAGATGTTTCTGAGCCAA  >  NC_002947/335657‑335942
                                                                                                                                              |                                                                                                                                               
ttCGGAAGTATCCTACGCGCGCCGCCGAAGTAACGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCt                                                                                                                                          >  1:106379/1‑150 (MQ=255)
  cGGAAGTATCCTACGCGCGCCGCCGAAGTAACGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCg                                                                                                                                        <  1:1163297/150‑1 (MQ=255)
  cGGAAGTATCCTACGCGCGCCGCCGAAGTAACGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCg                                                                                                                                        <  1:1271537/150‑1 (MQ=255)
          tCCTACGCGCGCCGCCGAAGTAACGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGcc                                                                                                                                >  2:1225155/1‑150 (MQ=255)
            cTACGCGCGCCGCCGAAGTAACGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACGCGACTTGAATAAGAGTTACACGCCATGGTGGTCGTACGTGGGCAGACTTGGGTCTGGCCgg                                                                                                                              >  2:541804/1‑150 (MQ=255)
            cTACGCGCGCCGCCGAAGTAACGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCgg                                                                                                                              <  2:106379/150‑1 (MQ=255)
                 cgaggcgcagAAGTAACGCTGTTCTTGGTGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTc                                                                                                                         <  1:541804/145‑1 (MQ=255)
                    gccgccGAAGTAACGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCtt                                                                                                                      <  1:482288/150‑1 (MQ=255)
                               aaCGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGt                                                                                                           <  2:819792/150‑1 (MQ=255)
                                  gCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTtc                                                                                                        >  2:352199/1‑150 (MQ=255)
                                  gCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTtc                                                                                                        >  1:312856/1‑150 (MQ=255)
                                      ttgtCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCAc                                                                                                    >  2:99449/1‑150 (MQ=255)
                                      ttgtCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCAc                                                                                                    >  1:779097/1‑150 (MQ=255)
                                               aaGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGCGGCCAGACCGAGGTCTgcccac                                                                                                                              >  2:872555/1‑110 (MQ=255)
                                               aaGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGCGGCCAGACCGAGGTCTgcccac                                                                                                                              <  1:872555/115‑6 (MQ=255)
                                                 gTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACgg                                                                                         >  2:807840/1‑150 (MQ=255)
                                                     cTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTgc                                                                                     <  2:1110914/150‑1 (MQ=255)
                                                      tCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTAt                                                                                                                    >  1:35948/1‑118 (MQ=255)
                                                      tCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTAt                                                                                                                    <  2:35948/118‑1 (MQ=255)
                                                           gATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGcccc                                                                               >  2:1022202/1‑150 (MQ=255)
                                                            aTACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCt                                                                                                 >  1:716483/1‑131 (MQ=255)
                                                            aTACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCt                                                                                                 <  2:716483/131‑1 (MQ=255)
                                                            aTACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCa                                                                              <  1:1090744/150‑1 (MQ=255)
                                                                cGTTTTCGGGTCGCTCTGGTGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATc                                                                          >  1:730082/1‑150 (MQ=255)
                                                                cGTTTTCGGGTCGCTCTGGCGACTGTGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATc                                                                          >  1:174435/1‑150 (MQ=255)
                                                                cGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATc                                                                          >  1:936400/1‑150 (MQ=255)
                                                                 gTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGttt                                                                                                         <  1:933543/118‑1 (MQ=255)
                                                                 gTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGttt                                                                                                         >  2:933543/1‑118 (MQ=255)
                                                                    ttCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTg                                                                      <  2:50611/150‑1 (MQ=255)
                                                                     tCGGGTCGCTCTGGTGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTga                                                                     <  2:730082/150‑1 (MQ=255)
                                                                       gggTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGt                                                                       >  1:392566/1‑146 (MQ=255)
                                                                       gggTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGt                                                                       <  2:392566/146‑1 (MQ=255)
                                                                                tGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGAttttt                                                          <  1:352199/150‑1 (MQ=255)
                                                                                tGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGCCAGACCGAGGTCTgcc                                                                                                                                 <  2:118245/79‑3 (MQ=255)
                                                                                tGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGCCAGACCGAGGTCTgcc                                                                                                                                 >  1:118245/1‑77 (MQ=255)
                                                                                  gCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGCCAGACCGAGGTCTGccca                                                                                                                               >  2:1002166/1‑78 (MQ=38)
                                                                                  gCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGCCAGACCGAGGTCTGccca                                                                                                                               <  1:1002166/79‑2 (MQ=38)
                                                                                           tgtgAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCt                                               >  1:1011435/1‑150 (MQ=255)
                                                                                             tgAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTc                                             >  2:450090/1‑150 (MQ=255)
                                                                                                gaACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGt                                          <  1:450090/150‑1 (MQ=255)
                                                                                                 aaCCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTg                                         <  1:99449/150‑1 (MQ=255)
                                                                                                    ccGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAg                                                     <  2:1076230/135‑1 (MQ=255)
                                                                                                    ccGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAg                                                     >  1:1076230/1‑135 (MQ=255)
                                                                                                      gACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATaa                                    <  2:936400/150‑1 (MQ=255)
                                                                                                      gACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATaa                                    <  1:1022202/150‑1 (MQ=255)
                                                                                                      gACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATACGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATaa                                    <  2:312856/150‑1 (MQ=255)
                                                                                                           gAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCg                                                                                                             >  1:947573/1‑72 (MQ=255)
                                                                                                           gAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCg                                                                                                             <  2:947573/72‑1 (MQ=255)
                                                                                                                   gTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCCATGa                       >  2:1257017/1‑150 (MQ=255)
                                                                                                                   gTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCCATGa                       >  1:920400/1‑150 (MQ=255)
                                                                                                                        ccGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGa                                        >  2:225915/1‑128 (MQ=255)
                                                                                                                        ccGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGa                                        <  1:225915/128‑1 (MQ=255)
                                                                                                                                        aCGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCCATGAAAAAAGATGTTTCTGAGCCaa  <  2:779097/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
TTCGGAAGTATCCTACGCGCGCCGCCGAAGTAACGCTGTTGTCGGGGAAGTGGCTCACGGATACCGTTTTCGGGTCGCTCTGGCGACTGGGTGTGAGAACCCGACTTGAATAAGCGTTACCCGCCATGGTGGTCGTACGTGGGCAGACCTCGGTCTGGCCGGGCTTCCTTATTCACTCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCCATGAAAAAAGATGTTTCTGAGCCAA  >  NC_002947/335657‑335942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑