Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,971 G→C 6.3% H271D (CAT→GAT)  PP_4563 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,9710GC 6.3% 252.1 / 6.9 80H271D (CAT→GAT) PP_4563hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (38/37);  new base C (2/3);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

CTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGAT  >  NC_002947/5182824‑5183118
                                                                                                                                                   |                                                                                                                                                    
cTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGa                                                                                                                                                    >  2:455616/1‑150 (MQ=255)
  ggCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATg                                                                                                                                                  >  1:1253534/1‑150 (MQ=255)
  ggCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATg                                                                                                                                                  <  2:1253534/150‑1 (MQ=255)
      tgcgcAGCTCCACCCTCCGCCTGGCCCTCAGCGCCGACCGGACCTCGCTGCTGCGCGACACCCGTCCGGGTGGCATGCCGCTGGACTTCCGCAACTTCGTAAATGGGTAATGCCGTTCGGCTGTCTCGGTACGATCGTCCCGGATCACGa                                                                                                                                              <  2:343463/149‑1 (MQ=255)
             cTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGAGTCAGGCCGTCAGGCTTTCTCGATGCGATCGTCATCCATGACGATcgcatc                                                                                                                                       <  2:501628/150‑6 (MQ=255)
              tCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGccc                                                                                                                                      >  2:813409/1‑150 (MQ=255)
                caccaTCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTg                                                                                                                                    >  2:647817/1‑150 (MQ=255)
                   caTCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCtt                                                                                                                                 >  1:404387/1‑150 (MQ=255)
                     tCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGATTGa                                                                                                                               <  2:660696/150‑1 (MQ=255)
                      cAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGaa                                                                                                                              <  2:1129958/150‑1 (MQ=255)
                           tGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAAcc                                                                                                                         <  2:1070652/150‑1 (MQ=255)
                               ccTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGcc                                                                                                                     >  1:852916/1‑150 (MQ=255)
                                           ccGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCcttctt                                                                                                         <  2:404387/150‑1 (MQ=255)
                                              ggACTCGCTGCTTCTCTACACCCGTCCTGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑aa                                                                                                     >  2:982578/1‑150 (MQ=255)
                                              ggACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑aa                                                                                                     <  2:626069/150‑1 (MQ=255)
                                                 cTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGtt                                                                                                  >  2:546593/1‑150 (MQ=255)
                                                  tCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTg                                                                                                 >  2:1173158/1‑150 (MQ=255)
                                                  tCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTg                                                                                                 >  1:624153/1‑150 (MQ=255)
                                                  tCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGAAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTg                                                                                                 >  1:553248/1‑150 (MQ=255)
                                                    gctgctGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCTGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGcc                                                                                               >  1:550487/1‑150 (MQ=255)
                                                    gctgctGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCCATGGACTTCTTG‑AATTTGcc                                                                                               >  2:713139/1‑150 (MQ=255)
                                                         tgcGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGc                                                                                          >  1:686718/1‑150 (MQ=255)
                                                           cgcgACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTg                                                                                        >  2:90432/1‑150 (MQ=255)
                                                             cgACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGAc                                                                                      <  1:468281/150‑1 (MQ=255)
                                                                cacCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGtt                                                                                   <  1:455616/150‑1 (MQ=255)
                                                                   ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGcc                                                                                                                                       <  2:636357/96‑1 (MQ=255)
                                                                   ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGcc                                                                                                                                       >  1:636357/1‑96 (MQ=255)
                                                                   ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGACATCTTG‑AAGTTGCCCTTGCTGACGTTGaa                                                                                >  1:839341/1‑150 (MQ=255)
                                                                      tCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAg                                                                             <  1:90432/150‑1 (MQ=255)
                                                                        cGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCt                                                                           <  1:1136030/150‑1 (MQ=255)
                                                                           gTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGc                                                                        >  1:4764/1‑150 (MQ=255)
                                                                            tGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCt                                                                       <  1:648877/150‑1 (MQ=255)
                                                                            tGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCt                                                                       >  2:212039/1‑150 (MQ=255)
                                                                              gCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGa                                                                     <  2:790020/150‑1 (MQ=255)
                                                                              cgcagcagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGa                                                                     <  1:96937/147‑1 (MQ=255)
                                                                                gAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATc                                                                   >  2:260920/1‑150 (MQ=255)
                                                                                   cagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAAc                                                                <  1:647817/150‑1 (MQ=255)
                                                                                     gcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑aa                                                                                                     >  1:160524/1‑111 (MQ=255)
                                                                                     gcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑aa                                                                                                     <  2:160524/111‑1 (MQ=255)
                                                                                      tccgatctCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAAT‑TCCTTGGGGTAATTTGCCCTTGCTGACGTTGAACAGTTTGCTGATCAACGCa                                                             <  1:863660/143‑1 (MQ=255)
                                                                                      cTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATg                                                                                                                 >  2:1085634/1‑99 (MQ=255)
                                                                                      cTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATg                                                                                                                 <  1:1085634/99‑1 (MQ=255)
                                                                                            ttacGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCa                                                       >  1:175856/4‑150 (MQ=255)
                                                                                            ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGaa                                                                                <  2:968867/125‑1 (MQ=255)
                                                                                            ttCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGaa                                                                                >  1:968867/1‑125 (MQ=255)
                                                                                               cGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGa                                                                     <  2:535016/133‑1 (MQ=255)
                                                                                               cGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGa                                                                     >  1:535016/1‑133 (MQ=255)
                                                                                               cGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActc                                                    >  2:666678/1‑150 (MQ=255)
                                                                                                gCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAAGAACCCGCCAATGGCATTCTTG‑AACTTGACATTGTTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActct                                                   >  1:725936/1‑150 (MQ=255)
                                                                                                gCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActct                                                   >  1:1238631/1‑150 (MQ=255)
                                                                                                gCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActct                                                   >  2:295109/1‑150 (MQ=255)
                                                                                                  aaCTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAACgtca                                                            >  2:412065/1‑136 (MQ=255)
                                                                                                  aaCTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAACgtca                                                            <  1:412065/139‑4 (MQ=255)
                                                                                                    cTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGc                                                                                                <  2:746302/101‑1 (MQ=255)
                                                                                                    cTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGc                                                                                                >  1:746302/1‑101 (MQ=255)
                                                                                                    cTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTc                                               <  2:852916/150‑1 (MQ=255)
                                                                                                      tCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGc                                             <  2:411264/150‑1 (MQ=255)
                                                                                                           cAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACAcc                                        <  1:713139/150‑1 (MQ=255)
                                                                                                             ggCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACAcccc                                      <  1:1173158/150‑1 (MQ=255)
                                                                                                                    ggCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACAcc                               <  1:763647/150‑1 (MQ=255)
                                                                                                                    ggCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACAcc                               >  1:379908/1‑150 (MQ=255)
                                                                                                                       cGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCg                            >  2:951351/1‑150 (MQ=255)
                                                                                                                         tCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCtt                                                  >  2:447868/1‑126 (MQ=255)
                                                                                                                         tCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCtt                                                  <  1:447868/126‑1 (MQ=255)
                                                                                                                          cAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCt                         <  2:4764/150‑1 (MQ=255)
                                                                                                                           aGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCtt                        >  1:1006736/1‑150 (MQ=255)
                                                                                                                           aGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCtt                        <  1:666678/150‑1 (MQ=255)
                                                                                                                             gCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCg                      <  2:175856/150‑1 (MQ=255)
                                                                                                                              cTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCgg                     >  2:292895/1‑150 (MQ=255)
                                                                                                                               tttCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGc                    <  1:546593/150‑1 (MQ=255)
                                                                                                                               tttCTCGAAGCGATGGTCATGGAAAACGATCAGGCTCTGGTTGAAAAACCGGCCAATGGCCTACTTG‑AAATTGCCGTTGCTGACGTTGAACAGCTTGATGATCAACGCAGGGTAACTCTTGTCGCACACCCCCCACACCCCTCCTTCCGc                    >  2:199287/1‑150 (MQ=255)
                                                                                                                               tgtgtcGATGCGATCGTCAAGGACGACGATCAGGCCCTGCTTAAACAACCCGCCAATGGCCCTCTTG‑AAGTTGCCCTTGCTGACGTTGCACAGCTTGCTGATCAACGCACTGTCACTCTTGTCGCACACCCCCAACACTCCGCCTTCGGc                    <  2:583657/146‑1 (MQ=255)
                                                                                                                                   tcGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTcc                >  2:334565/1‑150 (MQ=255)
                                                                                                                                      gcctgATCGTCATCCATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActc                                                    >  2:285835/5‑111 (MQ=255)
                                                                                                                                      gcctgATCGTCATCCATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActc                                                    <  1:285835/107‑1 (MQ=255)
                                                                                                                                          gATCGTCATCCATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAggg                                                          >  1:110167/1‑101 (MQ=255)
                                                                                                                                          gATCGTCATCCATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAggg                                                          <  2:110167/101‑1 (MQ=255)
                                                                                                                                             cGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCa      <  2:1006736/150‑1 (MQ=255)
                                                                                                                                               tCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATg    <  1:260920/150‑1 (MQ=255)
                                                                                                                                                 aTGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGAt  <  1:951351/150‑1 (MQ=255)
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CTGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTG‑AAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGCCATGAT  >  NC_002947/5182824‑5183118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑