Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,194,935	T→G	12.5%	intergenic (+29/+24)	PP_4574 → / ← PP_4575	hypothetical protein/urea amidolyase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,194,935	0	T	G	12.5%	66.0 / 5.8	32	intergenic (+29/+24)	PP_4574/PP_4575	hypothetical protein/urea amidolyase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (12/16);  new base G (2/2);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-01

CCCAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCGC  >  NC_002947/5194787‑5195084                                                                                                                                                     |                                                                                                                                                      cccAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAGGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTc                                                                                                                                                      >  1:334620/1‑150 (MQ=255)   cAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGc                                                                                                                                                    <  2:666905/150‑1 (MQ=255)       cAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCcgc                                                                                                                                                <  2:798357/150‑1 (MQ=255)           ccTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTTCACTTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                            <  1:936801/150‑1 (MQ=255)                   tACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCcagc                                                                                                                                    >  2:260410/1‑150 (MQ=255)                              gACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGAc                                                                                                                         <  1:260410/150‑1 (MQ=255)                                          gcgcGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                              <  1:202641/149‑1 (MQ=255)                                          gcgcGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                              >  2:202641/1‑149 (MQ=255)                                                       gAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctgctgcc                                                                                                >  2:608523/1‑149 (MQ=255)                                                        aGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGAc                                                                                                                         >  1:51107/1‑124 (MQ=255)                                                        aGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGAc                                                                                                                         <  2:51107/124‑1 (MQ=255)                                                            cAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGGATTACGACCAACGCCCCAGGTAAAGCTGCTGGTCTCGc                                                                                           >  2:112617/1‑150 (MQ=255)                                                             aaGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTa                                                                                                                             >  1:142036/1‑115 (MQ=255)                                                             aaGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTa                                                                                                                             <  2:142036/115‑1 (MQ=255)                                                              aGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCCGGTAAAGCTGCTGCTCTCGCCa                                                                                         <  2:1244676/150‑1 (MQ=255)                                                               gATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                              <  1:756269/128‑1 (MQ=255)                                                               gATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                              >  2:756269/1‑128 (MQ=255)                                                               gATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAg                                                                                        <  1:646323/150‑1 (MQ=255)                                                                        gCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGc                                                                                                                                                    <  1:598536/81‑1 (MQ=255)                                                                        gCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGc                                                                                                                                                    >  2:598536/1‑81 (MQ=255)                                                                                tGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGcaccac                                                                       <  2:334620/150‑1 (MQ=255)                                                                                tGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGcaccac                                                                       >  2:835254/1‑150 (MQ=255)                                                                                ggCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGACTCGCCCGCGAAAGTGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGcaccac                                                                       <  2:198592/149‑1 (MQ=255)                                                                                          ggaggaCGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAAc                                                             <  1:835254/150‑1 (MQ=255)                                                                                                                   aaGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGcacca                                                                        <  2:539454/114‑1 (MQ=255)                                                                                                                   aaGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGcacca                                                                        >  1:539454/1‑114 (MQ=255)                                                                                                                              ccTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgc                         <  2:1006241/150‑1 (MQ=255)                                                                                                                                          cTTTCGCGGGCGAGCCCGCGAAAGGGCCGGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCgccagcgccagc             >  1:279838/1‑150 (MQ=255)                                                                                                                                           tttCGCGGGCGAGCCCGCGAAAGGGCCGGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCaccacc                                                                      >  1:753464/1‑92 (MQ=38)                                                                                                                                           tttCGCGGGCGAGCCCGCGAAAGGGCCGGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCaccacc                                                                      <  2:753464/92‑1 (MQ=38)                                                                                                                                            ttCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAg                                                                                        <  1:692589/73‑1 (MQ=255)                                                                                                                                            ttCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAg                                                                                        >  2:692589/1‑73 (MQ=255)                                                                                                                                                     tCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAgcgc  >  1:823378/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CCCAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCGC  >  NC_002947/5194787‑5195084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑