Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,199,693 G→C 7.9% intergenic (+20/+53) PP_4579 → / ← PP_4580 LysR family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,199,6930GC 7.9% 150.8 / 5.3 51intergenic (+20/+53)PP_4579/PP_4580LysR family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/26);  new base C (2/2);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

CTTGAGCTGGTGGTGGCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGGCCAGCA  >  NC_002947/5199551‑5199837
                                                                                                                                              |                                                                                                                                                
cTTGAGCTGGTGGTGGCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCt                                                                                                                                           >  2:1144596/1‑150 (MQ=255)
 ttGAGCTGGTGGTGGCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCtt                                                                                                                                          >  2:862109/1‑150 (MQ=255)
 ttGAGCTGGTGGTGACGTGGCAGACCGGGGTGGCGTTTGTCGATGAGGTGGTGGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCtt                                                                                                                                          >  2:471896/1‑150 (MQ=255)
   gAGCTGGTGGTGGCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTc                                                                                                                                        <  1:295874/150‑1 (MQ=255)
          tggtggCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCa                                                                                                                                 <  2:29620/150‑1 (MQ=255)
           ggtggCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCAt                                                                                                                                >  2:1017366/1‑150 (MQ=255)
                  tggcAGACCGGGGTGGCGTTGGTCGAAGAGGTGGTTGGGGTTTGCCGGCAGGTGTTAGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCCTTCGCGGGCATGACcgcg                                                                                                                         >  2:1228519/1‑150 (MQ=255)
                      aGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAg                                                                                                                     >  2:8432/1‑150 (MQ=255)
                       gACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCGCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAgg                                                                                                                    <  2:61960/150‑1 (MQ=255)
                          cGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGcc                                                                                                                 <  2:1032326/150‑1 (MQ=255)
                                  cGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCggtacagg                                                                                                         <  1:507436/150‑1 (MQ=255)
                                   gTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGTGCCggtacaggt                                                                                                        >  2:931175/1‑150 (MQ=255)
                                    ttGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAgg                                                                                                                    >  1:219730/1‑137 (MQ=255)
                                    ttGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAgg                                                                                                                    <  2:219730/137‑1 (MQ=255)
                                          gATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCACCTGTACCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCgctac                                                                                                            >  2:290228/1‑139 (MQ=255)
                                          gATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCACCTGTACCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCgctac                                                                                                            <  1:290228/139‑1 (MQ=255)
                                                gtggtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTa                                                                                           <  2:1208730/150‑1 (MQ=255)
                                                 tggtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGTCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAg                                                                                          <  1:97038/150‑1 (MQ=255)
                                                 tggtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAg                                                                                          <  1:8432/150‑1 (MQ=255)
                                                 tggtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAg                                                                                          <  1:1144596/150‑1 (MQ=255)
                                                  ggtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCTGGCATGCCCGCGAAAGGGCCggtacag                                                                                                          <  2:972915/133‑1 (MQ=255)
                                                  ggtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCTGGCATGCCCGCGAAAGGGCCggtacag                                                                                                          >  1:972915/1‑133 (MQ=255)
                                                   gtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAgcg                                                                                        <  2:259886/150‑1 (MQ=255)
                                                           ttGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGctct                                                                                >  1:327258/1‑150 (MQ=255)
                                                            tGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGctctc                                                                               <  1:1105952/150‑1 (MQ=255)
                                                                  cAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTcac                                                                         <  1:1017366/150‑1 (MQ=255)
                                                                   aGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTcacc                                                                        >  2:892316/1‑150 (MQ=255)
                                                                   aGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCCAAAGGGCCGGTACAGGGACAGCAGAACTTAGCGCCAGCTCTCTTTcacc                                                                        >  1:580588/1‑150 (MQ=255)
                                                                     gtgtTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTcaccgc                                                                      >  2:643120/1‑148 (MQ=255)
                                                                     gtgtTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTACCGGcc                                                                                                                                             >  2:30663/1‑79 (MQ=255)
                                                                     gtgtTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTACCGGcc                                                                                                                                             <  1:30663/79‑1 (MQ=255)
                                                                                      gcgGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCa                                                     >  2:899114/1‑150 (MQ=255)
                                                                                       gggtaTGGGGGGGGGCAGCGGATCGTGCTGGTCCGACTAACAGTCTCCGTCTGTAGCGTTCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAg                                                    <  2:235948/146‑1 (MQ=255)
                                                                                       cgGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAg                                                    <  2:327258/150‑1 (MQ=255)
                                                                                       cgGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAg                                                    <  1:279089/150‑1 (MQ=255)
                                                                                            tgtgGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATca                                               <  1:471896/150‑1 (MQ=255)
                                                                                             gtgggtggGCAGCGGATCGGGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATcag                                              <  1:138380/150‑1 (MQ=255)
                                                                                                ggtgggCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCAAGTGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATcagcca                                           <  1:862109/150‑1 (MQ=255)
                                                                                                         cGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGGAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATccc                                  >  1:680651/1‑150 (MQ=255)
                                                                                                                 gCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACa                          <  2:510091/150‑1 (MQ=255)
                                                                                                                      tCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTAcagc                                                                                                   >  2:287600/1‑72 (MQ=255)
                                                                                                                      tCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTAcagc                                                                                                   <  1:287600/72‑1 (MQ=255)
                                                                                                                          aCCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCCGCCACTCCCCAGCAACAGGCTTTCGa                 >  2:868464/1‑150 (MQ=255)
                                                                                                                          aCCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGa                 >  2:133806/1‑150 (MQ=255)
                                                                                                                           ccAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGAc                <  1:899114/150‑1 (MQ=255)
                                                                                                                            cAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGAcc               >  2:709572/1‑150 (MQ=255)
                                                                                                                                  tctcCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAg         <  1:892316/150‑1 (MQ=255)
                                                                                                                                  tctcCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAg         <  1:133806/150‑1 (MQ=255)
                                                                                                                                       gCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTc                   <  1:797143/135‑1 (MQ=255)
                                                                                                                                       gCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTc                   >  2:797143/1‑135 (MQ=255)
                                                                                                                                         cTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGGCCAGca  >  1:414160/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                
CTTGAGCTGGTGGTGGCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGGCCAGCA  >  NC_002947/5199551‑5199837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑