Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,239,932	G→A	12.5%	A144V (GCG→GTG)	ldh ←	leucine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,239,932	0	G	A	12.5%	160.1 / 13.1	64	A144V (GCG→GTG)	ldh	leucine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (25/31);  new base A (4/4);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

CAGCTCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGC  >  NC_002947/5239784‑5240077                                                                                                                                                     |                                                                                                                                                  cagcTCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGCCCGCTACCCGCAAGTATTTCTGCTTGCCGTGGGCTGGCCTGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATcgccgc                                                                                                                                                  >  1:151877/1‑150 (MQ=255)       cgcgCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTgg                                                                                                                                            <  2:838445/150‑1 (MQ=255)        gcgcCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCTCATCGCCACATGCGGc                                                                                                                                           >  1:1246330/1‑150 (MQ=255)            ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATg                                                                                                                                       >  1:649855/1‑150 (MQ=255)              aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATggg                                                                                                                                     >  2:389935/1‑150 (MQ=255)               ccGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCTCATCGCCACATGCGGCGATgggg                                                                                                                                    <  2:1246330/150‑2 (MQ=255)                     gCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCg                                                                                                                              >  1:909122/1‑150 (MQ=255)                     gCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCg                                                                                                                              >  1:185553/1‑150 (MQ=255)                       aaTTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGAt                                                                                                                            <  2:649855/150‑1 (MQ=255)                        aTTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATg                                                                                                                           >  2:101126/1‑150 (MQ=255)                           gCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAg                                                                                                                        <  2:442281/150‑1 (MQ=255)                               cGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAgtggt                                                                                                                    <  2:1162132/150‑1 (MQ=255)                                 ccAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGc                                                                                                                  <  2:659332/150‑1 (MQ=255)                                      gCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTc                                                                                                             <  2:543855/150‑1 (MQ=255)                                        aTAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCAc                                                                                                           <  1:101126/150‑1 (MQ=255)                                           aCCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTa                                                                                                        >  2:1283323/1‑150 (MQ=255)                                           aCCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACCTa                                                                                                        <  1:1283323/150‑1 (MQ=255)                                             cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTa                                                                                                        <  2:69667/148‑1 (MQ=255)                                             cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTa                                                                                                        >  1:69667/1‑148 (MQ=255)                                             cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAgg                                                                                                      >  2:1167581/1‑150 (MQ=255)                                             cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACACCCATCGCCACATGCGGCGATgggg                                                                                                                                    >  2:941104/1‑119 (MQ=255)                                             cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACACCCATCGCCACATGCGGCGATgggg                                                                                                                                    <  1:941104/120‑2 (MQ=255)                                               aCATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGc                                                                                                    <  2:136627/150‑1 (MQ=255)                                                 aTTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGt                                                                                                  >  1:831778/1‑150 (MQ=255)                                                 aTTGCACACCACCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGt                                                                                                  <  2:32424/150‑1 (MQ=255)                                                  ttGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGGGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGAAGTGGTGCTGGTCACGTAGGGCGTg                                                                                                 <  2:40479/150‑1 (MQ=255)                                                      ccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgt                                                                                             <  2:989490/150‑1 (MQ=255)                                                      ccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATAGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgt                                                                                             >  2:988461/1‑150 (MQ=255)                                                        aaCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                           <  1:1159224/150‑1 (MQ=255)                                                        aaCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                           <  2:210260/150‑1 (MQ=255)                                                             cTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAAt                                                                                      >  1:895357/1‑150 (MQ=255)                                                                gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCa                                                                                   >  1:217311/1‑150 (MQ=255)                                                                   cGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTc                                                                                <  1:205413/150‑1 (MQ=255)                                                                     cTACCCGCAAGCCTCTGAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCAGCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCa                                                                              <  2:151877/150‑1 (MQ=255)                                                                     cTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCa                                                                              >  1:609086/1‑150 (MQ=255)                                                                      tACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCAt                                                                             >  1:196054/1‑150 (MQ=255)                                                                      tACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACACCCATCGCCACATGCGGCGATgggggt                                                                                                                                  <  2:938484/97‑4 (MQ=38)                                                                      tACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAAGTTGTGGCACGTATGCCCGCGAACACACCCATCGCCACATGCGGCGATgggggt                                                                                                                                  >  1:938484/1‑94 (MQ=38)                                                                       aCCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGGATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGAAGTGCTTAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATg                                                                            <  1:32922/150‑1 (MQ=255)                                                                         ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGc                                                                                                    >  2:163755/1‑124 (MQ=255)                                                                         ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGc                                                                                                    <  1:163755/124‑1 (MQ=255)                                                                         ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTc                                                                          >  2:166822/1‑150 (MQ=255)                                                                            cAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCaa                                                                       <  1:389935/150‑1 (MQ=255)                                                                                   ctcATGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAg                                                                <  2:909122/150‑1 (MQ=255)                                                                                   ctcAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAg                                                                <  2:217311/150‑1 (MQ=255)                                                                                           aTCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACt                                                        >  2:302776/1‑150 (MQ=255)                                                                                                       ccGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACGGGCCACGGCgat                                            <  2:185553/150‑1 (MQ=255)                                                                                                          gAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                           >  1:283643/1‑100 (MQ=255)                                                                                                          gAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                           <  2:283643/100‑1 (MQ=255)                                                                                                                  ttGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGcc                                 <  1:1167581/150‑1 (MQ=255)                                                                                                                     tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                             >  2:370187/1‑135 (MQ=255)                                                                                                                     tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                             <  1:370187/135‑1 (MQ=255)                                                                                                                             aTGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTa                      >  1:975787/1‑150 (MQ=255)                                                                                                                                 ccGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCACTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGa                  <  1:175695/150‑1 (MQ=255)                                                                                                                                          gacccATCGCCACATGCGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCa                               >  1:709516/3‑128 (MQ=255)                                                                                                                                          gacccATCGCCACATGCGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCa                               <  2:709516/126‑1 (MQ=255)                                                                                                                                           ccccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGCCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACGGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGcc        >  2:861730/1‑150 (MQ=255)                                                                                                                                           ccccATCGCCGCATGTGGCGATGGGGCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGATGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGcc        >  1:808222/1‑150 (MQ=255)                                                                                                                                            cccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa       >  2:1042032/1‑150 (MQ=255)                                                                                                                                             ccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCaa      >  2:861576/1‑150 (MQ=255)                                                                                                                                              cATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCaa                          <  2:411622/129‑1 (MQ=255)                                                                                                                                              cATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCaa                          >  1:411622/1‑129 (MQ=255)                                                                                                                                              cATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCaaa     <  1:302776/150‑1 (MQ=255)                                                                                                                                               aTCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCCCCGGCCATTGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAg    <  1:673989/150‑1 (MQ=255)                                                                                                                                               aTCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGACACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAg    <  2:831778/150‑1 (MQ=255)                                                                                                                                                 cgccgcATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGc  <  1:861576/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CAGCTCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGC  >  NC_002947/5239784‑5240077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑