Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,239,937	T→C	12.3%	P142P (CCA→CCG)	ldh ←	leucine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,239,937	0	T	C	12.3%	166.4 / 13.2	65	P142P (CCA→CCG)	ldh	leucine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (25/32);  new base C (4/4);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

CGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGC  >  NC_002947/5239790‑5240077                                                                                                                                                    |                                                                                                                                             cgcgCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTgg                                                                                                                                            <  2:838445/150‑1 (MQ=255)  gcgcCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCTCATCGCCACATGCGGc                                                                                                                                           >  1:1246330/1‑150 (MQ=255)      ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATg                                                                                                                                       >  1:649855/1‑150 (MQ=255)        aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATggg                                                                                                                                     >  2:389935/1‑150 (MQ=255)         ccGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCTCATCGCCACATGCGGCGATgggg                                                                                                                                    <  2:1246330/150‑2 (MQ=255)               gCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCg                                                                                                                              >  1:909122/1‑150 (MQ=255)               gCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCg                                                                                                                              >  1:185553/1‑150 (MQ=255)                 aaTTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGAt                                                                                                                            <  2:649855/150‑1 (MQ=255)                  aTTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATg                                                                                                                           >  2:101126/1‑150 (MQ=255)                     gCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAg                                                                                                                        <  2:442281/150‑1 (MQ=255)                         cGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAgtggt                                                                                                                    <  2:1162132/150‑1 (MQ=255)                           ccAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGc                                                                                                                  <  2:659332/150‑1 (MQ=255)                                gCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTc                                                                                                             <  2:543855/150‑1 (MQ=255)                                  aTAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCAc                                                                                                           <  1:101126/150‑1 (MQ=255)                                     aCCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTa                                                                                                        >  2:1283323/1‑150 (MQ=255)                                     aCCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACCTa                                                                                                        <  1:1283323/150‑1 (MQ=255)                                       cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTa                                                                                                        <  2:69667/148‑1 (MQ=255)                                       cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTa                                                                                                        >  1:69667/1‑148 (MQ=255)                                       cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAgg                                                                                                      >  2:1167581/1‑150 (MQ=255)                                       cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACACCCATCGCCACATGCGGCGATgggg                                                                                                                                    >  2:941104/1‑119 (MQ=255)                                       cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACACCCATCGCCACATGCGGCGATgggg                                                                                                                                    <  1:941104/120‑2 (MQ=255)                                         aCATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGc                                                                                                    <  2:136627/150‑1 (MQ=255)                                           aTTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGt                                                                                                  >  1:831778/1‑150 (MQ=255)                                           aTTGCACACCACCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGt                                                                                                  <  2:32424/150‑1 (MQ=255)                                            ttGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGGGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGAAGTGGTGCTGGTCACGTAGGGCGTg                                                                                                 <  2:40479/150‑1 (MQ=255)                                                ccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgt                                                                                             <  2:989490/150‑1 (MQ=255)                                                ccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATAGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgt                                                                                             >  2:988461/1‑150 (MQ=255)                                                  aaCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                           <  1:1159224/150‑1 (MQ=255)                                                  aaCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                           <  2:210260/150‑1 (MQ=255)                                                       cTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAAt                                                                                      >  1:895357/1‑150 (MQ=255)                                                          gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCa                                                                                   >  1:217311/1‑150 (MQ=255)                                                             cGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTc                                                                                <  1:205413/150‑1 (MQ=255)                                                               cTACCCGCAAGCCTCTGAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCAGCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCa                                                                              <  2:151877/150‑1 (MQ=255)                                                               cTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCa                                                                              >  1:609086/1‑150 (MQ=255)                                                                tACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCAt                                                                             >  1:196054/1‑150 (MQ=255)                                                                tACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACACCCATCGCCACATGCGGCGATgggggt                                                                                                                                  <  2:938484/97‑4 (MQ=38)                                                                tACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAAGTTGTGGCACGTATGCCCGCGAACACACCCATCGCCACATGCGGCGATgggggt                                                                                                                                  >  1:938484/1‑94 (MQ=38)                                                                 aCCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGGATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGAAGTGCTTAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATg                                                                            <  1:32922/150‑1 (MQ=255)                                                                   ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGc                                                                                                    >  2:163755/1‑124 (MQ=255)                                                                   ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGc                                                                                                    <  1:163755/124‑1 (MQ=255)                                                                   ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTc                                                                          >  2:166822/1‑150 (MQ=255)                                                                      cAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCaa                                                                       <  1:389935/150‑1 (MQ=255)                                                                             ctcATGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAg                                                                <  2:909122/150‑1 (MQ=255)                                                                             ctcAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAg                                                                <  2:217311/150‑1 (MQ=255)                                                                                     aTCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACt                                                        >  2:302776/1‑150 (MQ=255)                                                                                                 ccGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACGGGCCACGGCgat                                            <  2:185553/150‑1 (MQ=255)                                                                                                    gAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                           >  1:283643/1‑100 (MQ=255)                                                                                                    gAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                           <  2:283643/100‑1 (MQ=255)                                                                                                            ttGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGcc                                 <  1:1167581/150‑1 (MQ=255)                                                                                                               tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                             >  2:370187/1‑135 (MQ=255)                                                                                                               tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                             <  1:370187/135‑1 (MQ=255)                                                                                                                       aTGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTa                      >  1:975787/1‑150 (MQ=255)                                                                                                                           ccGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCACTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGa                  <  1:175695/150‑1 (MQ=255)                                                                                                                                    gacccATCGCCACATGCGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCa                               >  1:709516/3‑128 (MQ=255)                                                                                                                                    gacccATCGCCACATGCGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCa                               <  2:709516/126‑1 (MQ=255)                                                                                                                                     ccccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGCCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACGGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGcc        >  2:861730/1‑150 (MQ=255)                                                                                                                                     ccccATCGCCGCATGTGGCGATGGGGCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGATGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGcc        >  1:808222/1‑150 (MQ=255)                                                                                                                                      cccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa       >  2:1042032/1‑150 (MQ=255)                                                                                                                                       ccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCaa      >  2:861576/1‑150 (MQ=255)                                                                                                                                        cATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCaa                          <  2:411622/129‑1 (MQ=255)                                                                                                                                        cATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCaa                          >  1:411622/1‑129 (MQ=255)                                                                                                                                        cATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCaaa     <  1:302776/150‑1 (MQ=255)                                                                                                                                         aTCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCCCCGGCCATTGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAg    <  1:673989/150‑1 (MQ=255)                                                                                                                                         aTCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGACACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAg    <  2:831778/150‑1 (MQ=255)                                                                                                                                           cgccgcATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGc  <  1:861576/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                             CGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGC  >  NC_002947/5239790‑5240077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑