| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,313,307 | A→G | 7.8% | intergenic (‑578/+136) | PP_16SG ← / ← msrQ | 16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,313,307 | 0 | A | G | 7.8% | 149.7 / 2.6 | 51 | intergenic (‑578/+136) | PP_16SG/msrQ | 16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base A (23/24); new base G (2/2); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01 | |||||||||||
AGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGC > NC_002947/5313159‑5313443 | aGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTAt < 2:808407/150‑1 (MQ=255)aGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTAt < 2:26482/150‑1 (MQ=255) ggAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATg > 2:1230834/1‑150 (MQ=255) tttttCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGAt > 2:1126404/1‑150 (MQ=255) ttCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGa > 1:749637/1‑150 (MQ=255) cGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAgc < 2:749637/150‑1 (MQ=255) gTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGCTG‑AAATACTGTATCGGGGCTTCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAgcg < 2:415164/150‑1 (MQ=255) gATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCgccgcc > 1:865948/1‑150 (MQ=255) tGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGccgcccg > 2:155604/1‑150 (MQ=255) aaGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCt < 1:1544/150‑1 (MQ=255) aGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTc < 1:1230834/150‑1 (MQ=255) cgcATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTca < 1:1126404/150‑1 (MQ=255) cATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTcaca > 1:1224591/1‑150 (MQ=255) taAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGc > 1:1219761/1‑150 (MQ=255) ggggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCgcggc > 1:328025/1‑129 (MQ=255) ggggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCgcggc < 2:328025/129‑1 (MQ=255) gagaGGGCGTCAACCTTTAATTTCAAGAAAGTGAAAGATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAAGAGTTTCAGCGCCTATGAGATCGAGCTCCGCCCGCGAGGCGCTCGATCTACCAGACGCCAGAGACACaa > 2:544136/1‑150 (MQ=17) gCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCgg < 2:1224591/150‑1 (MQ=255) tCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAAAGACCGTATCGGGGCTGCCAAGTGTTCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCg > 1:597372/1‑118 (MQ=11) tCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAAAGACCGTATCGGGGCTGCCAAGTGTTCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCg < 2:597372/118‑1 (MQ=11) cTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCcgcgcg > 2:843095/1‑105 (MQ=255) cTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCcgcgcg < 1:843095/105‑1 (MQ=255) cTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCagag < 2:958047/130‑1 (MQ=255) cTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCagag > 1:958047/1‑130 (MQ=255) tttCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAAAAACAAAGGCGGGGCCTCTCcacatc > 2:1038085/1‑148 (MQ=255) cAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCAt < 1:662374/150‑1 (MQ=255) aaGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCAtt > 1:1247830/1‑150 (MQ=255) gAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGc < 1:425274/107‑1 (MQ=255) gAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGc > 2:425274/1‑107 (MQ=255) gAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAACGGCGGGCCCTCTCCCCAGCCATTGCTAtctc > 1:21927/1‑150 (MQ=255) tataAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAt > 2:1185252/1‑143 (MQ=255) tataAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAt < 1:1185252/143‑1 (MQ=255) tataAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGa < 1:155604/150‑1 (MQ=255) ataAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGAc < 2:865948/150‑1 (MQ=255) aaGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGAGCTCGATCTAACAGACGCCAGAAACACAAAGGCGGTGCCTCTCCACAGCCATTGCTATCTCAAGACAg < 1:544136/150‑1 (MQ=25) g‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCt > 2:1026450/1‑150 (MQ=255) g‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCt > 2:941753/1‑150 (MQ=255) aTACCGTATCGGGGCCGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTgg < 2:892123/150‑1 (MQ=255) gaCCGTATCGGGGCTGCCAAGTGTTCGCCACGACAGTTTCAGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAggc < 2:1152692/88‑3 (MQ=1) gaCCGTATCGGGGCTGCCAAGTGTTCGCCACGACAGTTTCAGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAggc > 1:1152692/2‑87 (MQ=1) cGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGc < 2:1247830/150‑1 (MQ=255) gTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGcc < 1:1026450/150‑1 (MQ=255) tATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTgg > 1:912646/1‑144 (MQ=255) tATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTgg < 2:912646/144‑1 (MQ=255) cAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACtctc > 2:1258965/1‑150 (MQ=255) gtgtTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCaaa < 2:1188919/150‑1 (MQ=255) ttCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACCGGCCCTTGGCAGCCCCACCCCACTCTCAAAccc > 2:336024/1‑149 (MQ=255) tCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGc < 2:1219761/150‑1 (MQ=255) aacaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAg > 2:578985/1‑90 (MQ=255) aacaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAg < 1:578985/90‑1 (MQ=255) aGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGc > 1:677029/1‑150 (MQ=255) | AGGAGTTTTTCGTTCCGATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGG‑AAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGC > NC_002947/5313159‑5313443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |