Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,325,559	T→A	16.1%	noncoding (133/149 nt)	PP_mr52 →	PrrF ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,325,559	0	T	A	16.1%	147.0 / 20.8	56	noncoding (133/149 nt)	PP_mr52	PrrF ncRNA
Reads supporting (aligned to +/- strand):  ref base T (26/21);  new base A (5/4);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

CGGGGCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAGCAGCAA  >  NC_002947/5325416‑5325702                                                                                                                                                |                                                                                                                                                cGGGGCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCg                                                                                                                                           >  1:1200905/1‑150 (MQ=255)  ggggCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCgg                                                                                                                                          <  2:477065/150‑1 (MQ=255)  ggggCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCgg                                                                                                                                          <  2:1268690/150‑1 (MQ=255)       aaaGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCtt                                                                                                                                     <  1:682473/150‑1 (MQ=255)                      tAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGt                                                                                                                                        <  2:45100/132‑1 (MQ=255)                      tAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGt                                                                                                                                        >  1:45100/1‑132 (MQ=255)                          tGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCttttt                                                                                                                  >  2:1176743/1‑149 (MQ=255)                               aTTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGAt                                                                                                             <  2:911718/150‑1 (MQ=255)                                      ttattatCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGcgcc                                                                                                      <  1:1079890/150‑1 (MQ=255)                                        attatCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAAATTGCCGGGTcaa                                                                                                                                     <  2:376512/117‑3 (MQ=255)                                        attatCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAAATTGCCGGGTcaa                                                                                                                                     >  1:376512/1‑115 (MQ=255)                                            tCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAAATTGCCGGGt                                                                                                                                        >  1:705659/1‑110 (MQ=255)                                            tCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAAATTGCCGGGt                                                                                                                                        <  2:705659/110‑1 (MQ=255)                                               cAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTgcg                                                                                             >  1:806062/1‑150 (MQ=255)                                                aaCTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCttttttt                                                                                                                                >  1:1033019/1‑114 (MQ=255)                                                aaCTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCttttttt                                                                                                                                <  2:1033019/114‑1 (MQ=255)                                                      tCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACaata                                                                                      >  1:328202/1‑150 (MQ=255)                                                         cgAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATc                                                                                   <  2:328202/150‑1 (MQ=255)                                                          gagaCTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAg                                                                                                          <  2:856011/126‑1 (MQ=255)                                                          gagaCTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAg                                                                                                          >  1:856011/1‑126 (MQ=255)                                                                 gTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTc                                                                                                                  <  1:592693/111‑1 (MQ=255)                                                                 gTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTc                                                                                                                  >  2:592693/1‑111 (MQ=255)                                                                     gATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCAt                                                                       >  2:1168040/1‑150 (MQ=255)                                                                      aTAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATc                                                                      >  1:1092870/1‑150 (MQ=255)                                                                         aGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCTCACAATAATCCTGCTCCGGCATcg                                                                     >  1:966268/1‑148 (MQ=255)                                                                         aGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATcg                                                                     <  2:966268/148‑1 (MQ=255)                                                                           cTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATcgccgc                                                                 >  1:169992/1‑150 (MQ=255)                                                                                  ccccTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTTCCTTTGCCTTTTCAGTCTCAGCGCCGCAGCTGCGCACACTAATCCTGCTCCGGCCTCCCCTCCgtgtgc                                                          >  2:390248/1‑148 (MQ=37)                                                                                    ccTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTTTTTTTTTTTTTTCAGGATCAGCGCCGCAGATGAGCACAATAATCCTGCTCCGGCATCGCCGTCGTGTAccc                                                        >  2:1054074/1‑149 (MQ=25)                                                                                    ccTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGGGCACAATAATCCTGCTCCCGCATCTCCGCCGTGTAcaa                                                        >  2:553924/1‑148 (MQ=255)                                                                                             gACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATc                                               >  2:368400/1‑150 (MQ=255)                                                                                             gACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATc                                               >  1:4992/1‑150 (MQ=255)                                                                                              aCTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCa                                              <  2:169992/150‑1 (MQ=255)                                                                                                  tcAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCgg                                                                          >  1:1112344/1‑118 (MQ=255)                                                                                                  tcAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCgg                                                                          <  2:1112344/118‑1 (MQ=255)                                                                                                            tcctcATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTc                                >  1:373077/1‑150 (MQ=255)                                                                                                             cctcATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATc                                                                      >  1:733687/1‑111 (MQ=255)                                                                                                             cctcATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATc                                                                      <  2:733687/111‑1 (MQ=255)                                                                                                               tcatcaGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGt                             <  2:373077/150‑1 (MQ=255)                                                                                                                catcaGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGtt                            <  2:352623/150‑1 (MQ=255)                                                                                                                       cTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTtcgctcgc                     <  2:807446/150‑1 (MQ=255)                                                                                                                            cACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCAcc                >  1:633222/1‑150 (MQ=255)                                                                                                                                   aaagACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGcc                                      <  2:209214/118‑1 (MQ=255)                                                                                                                                   aaagACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGcc                                      >  1:209214/4‑121 (MQ=255)                                                                                                                                   aaagACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGcc                    <  1:911876/136‑1 (MQ=255)                                                                                                                                   aaagACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGcc                    >  2:911876/4‑139 (MQ=255)                                                                                                                                      gACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAg                                                                                                          >  1:630176/1‑50 (MQ=255)                                                                                                                                      gACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAg                                                                                                          <  2:630176/50‑1 (MQ=255)                                                                                                                                      gACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCcagca      >  1:1108945/1‑150 (MQ=255)                                                                                                                                        cccGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCagcagc    <  2:1092870/150‑1 (MQ=255)                                                                                                                                         ccGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAgcagca   >  1:1015446/1‑150 (MQ=255)                                                                                                                                         ccGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAgcagca   >  2:814400/1‑150 (MQ=255)                                                                                                                                          cGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGcc                    >  1:1136620/1‑132 (MQ=255)                                                                                                                                          cGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGcc                    <  2:1136620/132‑1 (MQ=255)                                                                                                                                          cGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAGCAGCaa  >  1:1152508/1‑150 (MQ=255)                                                                                                                                          cGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAGCAGCaa  <  2:1200905/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                CGGGGCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAGCAGCAA  >  NC_002947/5325416‑5325702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑