Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,338,098 C→G 7.4% intergenic (+57/‑227) PP_4696 → / → PP_mr53 nitrogen regulation protein NR(I)/CrcZ ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,338,0980CG 7.4% 168.1 / 5.2 54intergenic (+57/‑227)PP_4696/PP_mr53nitrogen regulation protein NR(I)/CrcZ ncRNA
Reads supporting (aligned to +/- strand):  ref base C (20/30);  new base G (2/2);  total (22/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

GCACGCAAGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCT  >  NC_002947/5337949‑5338245
                                                                                                                                                     |                                                                                                                                                   
gCACGCAAGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCgccgcc                                                                                                                                                     >  2:627693/1‑150 (MQ=255)
  aCGCAAGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGccgcccg                                                                                                                                                   >  1:1233143/1‑150 (MQ=255)
      aaGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCcgcgcg                                                                                                                                               <  1:627693/150‑1 (MQ=255)
       aGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCgcgg                                                                                                                                              <  2:1233143/150‑1 (MQ=255)
       aGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCgcgg                                                                                                                                              <  1:845983/150‑1 (MQ=255)
          tCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGgcgc                                                                                                                                           >  2:485145/1‑150 (MQ=255)
            ggCGTAAGCCGCGAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                         <  1:183465/150‑1 (MQ=255)
            ggCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCCACCCTACCTGCATTTTGCCCACTGAGTCACCTCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                         <  1:1120329/150‑1 (MQ=255)
             gCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                                        <  2:79826/150‑1 (MQ=255)
                 aaGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCt                                                                                                                                    <  1:804765/150‑1 (MQ=255)
                    ccGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTcac                                                                                                                                 >  1:46341/1‑150 (MQ=255)
                        tgttaccTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGgc                                                                                                                             >  2:411047/6‑150 (MQ=255)
                          gTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGgcgc                                                                                                                           <  2:730899/150‑1 (MQ=255)
                           tGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCt                                                                                                                          <  1:660539/150‑1 (MQ=255)
                              cTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGaa                                                                                                                       >  1:758239/1‑150 (MQ=255)
                              cTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGaa                                                                                                                       >  1:4208/1‑150 (MQ=255)
                              cTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCCCGGCCTCACAGGCGCTGaa                                                                                                                       >  2:214379/1‑150 (MQ=255)
                                   ggAACGCCGCCGGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACgtgt                                                                                                                  >  2:705435/1‑150 (MQ=255)
                                    gAACGCCGCCCGCGCCTGGGCAGCCCCCGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGTGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTc                                                                                                                 <  2:477267/150‑1 (MQ=255)
                                         ccgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGACACCAGGCGCCGCCCGCTCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacgac                                                                                                            <  1:349312/150‑1 (MQ=255)
                                          cgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacgacg                                                                                                           >  1:1006684/1‑150 (MQ=255)
                                             cAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGc                                                                                                                                      >  1:1213430/1‑120 (MQ=255)
                                             cAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGc                                                                                                                                      <  2:1213430/120‑1 (MQ=255)
                                                     gggCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAgg                                                                                                <  2:1006684/150‑1 (MQ=255)
                                                          tCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAaca                                                                                           <  1:411047/150‑1 (MQ=255)
                                                                   gcAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGGCGGCGCTCg                                                                                                                                        >  2:129021/1‑96 (MQ=38)
                                                                   gcAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGGCGGCGCTCg                                                                                                                                        <  1:129021/96‑1 (MQ=38)
                                                                    cAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTcc                                                                                 >  1:458973/1‑150 (MQ=255)
                                                                     aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGc                                                                                                                                      >  2:1107131/1‑96 (MQ=255)
                                                                     aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGc                                                                                                                                      <  1:1107131/96‑1 (MQ=255)
                                                                     aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                <  2:458973/150‑1 (MQ=255)
                                                                     aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                <  1:485145/150‑1 (MQ=255)
                                                                           aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCGCGGGCGGCGCTCg                                                                                                                                        >  1:716121/1‑88 (MQ=255)
                                                                           aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCGCGGGCGGCGCTCg                                                                                                                                        <  2:716121/88‑1 (MQ=255)
                                                                           aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGc                                                                                                                                      >  2:559269/1‑90 (MQ=255)
                                                                           aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGc                                                                                                                                      <  1:559269/90‑1 (MQ=255)
                                                                           aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGc                                                                                                                                      >  2:1201497/1‑90 (MQ=255)
                                                                           aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGc                                                                                                                                      <  1:1201497/90‑1 (MQ=255)
                                                                           aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGACTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTaaaa                                                                          >  2:931426/1‑150 (MQ=255)
                                                                                tACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTg                                                                     <  2:4208/150‑1 (MQ=255)
                                                                                 aCCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGt                                                                    <  2:46341/150‑1 (MQ=255)
                                                                                          tAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGtt                                                                   <  2:808333/142‑1 (MQ=255)
                                                                                          tAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGtt                                                                   >  1:808333/1‑142 (MQ=255)
                                                                                              ccACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGcc                                                                                                                                     >  2:1260903/1‑72 (MQ=255)
                                                                                              ccACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGcc                                                                                                                                     <  1:1260903/72‑1 (MQ=255)
                                                                                                      gcaccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTaa                                               <  1:1268342/150‑1 (MQ=255)
                                                                                                       caccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACACAAGCTTTCCGCCGTAAc                                              <  1:705435/150‑1 (MQ=255)
                                                                                                          cgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAacac                                                                                          <  2:1145253/103‑1 (MQ=255)
                                                                                                          cgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAacac                                                                                          >  1:1145253/1‑103 (MQ=255)
                                                                                                           gcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAcc                                                                >  1:572098/1‑128 (MQ=255)
                                                                                                           gcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAcc                                                                <  2:572098/128‑1 (MQ=255)
                                                                                                             acGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTccc                                        <  2:1283332/150‑1 (MQ=255)
                                                                                                               gccgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTcccc                                       <  2:1026965/149‑1 (MQ=255)
                                                                                                               gccgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTcccc                                       >  1:1026965/1‑149 (MQ=255)
                                                                                                                ccgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAGTTCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGa                                     <  1:214379/150‑1 (MQ=255)
                                                                                                                                    gTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTg                 >  1:651719/1‑150 (MQ=255)
                                                                                                                                                   gcccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCt  <  1:109491/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCACGCAAGCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCT  >  NC_002947/5337949‑5338245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑