Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,352,028 A→C 8.2% intergenic (‑233/+31) PP_4707 ← / ← pnp OsmY‑like protein/polyribonucleotide nucleotidyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,352,0280AC 8.2% 130.4 / 5.2 49intergenic (‑233/+31)PP_4707/pnpOsmY‑like protein/polyribonucleotide nucleotidyltransferase
Reads supporting (aligned to +/- strand):  ref base A (20/25);  new base C (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

GCTTCTGTTATTTCCTTTTAAATCAATAAGTTAGCAAATATCTATCACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTGGCCTTCCTTGAGGATGTCGGTGA  >  NC_002947/5351884‑5352177
                                                                                                                                                |                                                                                                                                                     
gctTCTGTTATTTCCTTTTAAATCAATAAGTTAGCAAATATCTATCACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGc                                                                                                                                                  <  1:854745/150‑1 (MQ=255)
   tCTGTTATTTCCTTTTAAATCAATAAGTTAGCAAATATCTATCACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCt                                                                                                                                               <  2:598276/150‑1 (MQ=255)
       ttATTTCCTTTTAAATCAATAAGTTAGCAAATATCTATCACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTtct                                                                                                                                           >  1:889736/1‑150 (MQ=255)
         aTTTCCTTTTAAATCAATAAGTTAGCAAATATCTATCACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCATCGATTGTCCCTTTctct                                                                                                                                         <  2:1017739/150‑1 (MQ=255)
                    aaTCAATAAGTTAGCAAATATCTATCACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACg                                                                                                                              >  2:1075216/1‑150 (MQ=255)
                       cAATAAGTTAGCAAATATCTATCACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTc                                                                                                                           <  1:1278685/150‑1 (MQ=255)
                            aGTTAGCAAATATCTATCACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTa                                                                                                                      <  2:966456/150‑1 (MQ=255)
                                        tctatcACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTTCGCAGGGCCCtttt                                                                                                                                            >  1:1236655/1‑115 (MQ=255)
                                        tctatcACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTTCGCAGGGCCCtttt                                                                                                                                            <  2:1236655/116‑2 (MQ=255)
                                         ctatcACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATTCAATTCCGCAAAAGTTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGcc                                                                                                         >  2:405609/1‑150 (MQ=255)
                                                ttCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGa                                                                                                                                >  2:356307/1‑120 (MQ=255)
                                                ttCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGa                                                                                                                                <  1:356307/120‑1 (MQ=255)
                                                          gTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAg                                                                                                                                                     <  1:75382/89‑1 (MQ=255)
                                                          gTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAg                                                                                                                                                     >  2:75382/1‑89 (MQ=255)
                                                                     aaTCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTtct                                                                                                                                           <  1:1264052/88‑1 (MQ=255)
                                                                     aaTCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTtct                                                                                                                                           >  2:1264052/1‑88 (MQ=255)
                                                                          gcgcGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGc                                                                                                                 >  1:661069/1‑109 (MQ=255)
                                                                          gcgcGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGc                                                                                                                 <  2:661069/109‑1 (MQ=255)
                                                                          gcgcGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTg                                                                        >  2:307146/1‑150 (MQ=255)
                                                                                    ccTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCcgc                                                              >  1:902443/1‑150 (MQ=255)
                                                                                     cTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCcgcg                                                             >  2:897189/1‑150 (MQ=255)
                                                                                         atgcaagacgcaaaCCCGTAAAAGTTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTg                                                         <  2:996949/150‑1 (MQ=255)
                                                                                          tgcaagatgcaagTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAgg                                                                                              >  2:908593/1‑112 (MQ=255)
                                                                                          tgcaagatgcaagTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAgg                                                                                              <  1:908593/112‑1 (MQ=255)
                                                                                           gcaagatgcaagTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTc                                                       <  2:889736/150‑1 (MQ=255)
                                                                                                 tgcaagTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTc                                                 <  1:897189/150‑1 (MQ=255)
                                                                                                       tCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCAc                                                    >  1:730448/1‑141 (MQ=255)
                                                                                                       tCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCAc                                                    <  2:730448/141‑1 (MQ=255)
                                                                                                       tCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTat                                           <  2:881780/150‑2 (MQ=255)
                                                                                                       tCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTAc                                           >  1:829020/1‑150 (MQ=255)
                                                                                                         cGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACaa                                         <  1:1075216/150‑1 (MQ=255)
                                                                                                                ggTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTc                                  >  2:854625/1‑150 (MQ=255)
                                                                                                                   tCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAAc                               <  2:1209355/150‑1 (MQ=255)
                                                                                                                   tCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAAc                               >  1:1209355/1‑150 (MQ=255)
                                                                                                                     ccGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCAc                                     <  2:129109/142‑1 (MQ=255)
                                                                                                                     ccGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCAc                                     >  1:129109/1‑142 (MQ=255)
                                                                                                                          cccATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCt                                                                                       <  2:1268987/87‑1 (MQ=255)
                                                                                                                          cccATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCt                                                                                       >  1:1268987/1‑87 (MQ=255)
                                                                                                                          cccATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTgg                        <  2:829020/150‑1 (MQ=255)
                                                                                                                           ccATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTGGc                       >  2:823711/1‑150 (MQ=255)
                                                                                                                             aTGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCAc                                     >  2:1241328/1‑134 (MQ=255)
                                                                                                                             aTGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCAc                                     <  1:1241328/134‑1 (MQ=255)
                                                                                                                              tGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTGGcctt                    >  1:354930/1‑150 (MQ=255)
                                                                                                                                aaaaaaGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTGGccttcc                  <  1:74486/150‑1 (MQ=255)
                                                                                                                                 aaaaaGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTGGccttcct                 <  2:902443/150‑1 (MQ=255)
                                                                                                                                   aaaGGGCCCTTCGCAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGtt                                                          >  1:567777/1‑107 (MQ=255)
                                                                                                                                   aaaGGGCCCTTCGCAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGtt                                                          <  2:567777/107‑1 (MQ=255)
                                                                                                                                    aaGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTCACGCCCGGATCGGGGCTCGAGTCCTTGGCCGCAGGGACGTTTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTGGCCTTCCTTGa              <  1:1249604/150‑1 (MQ=255)
                                                                                                                                              cGAAGGGCCCTTTCTCTATTGCGTGTCGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTGGCCTTCCTTGAGGATGTCGGt    <  2:350126/150‑1 (MQ=255)
                                                                                                                                                aaGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTGGCCTTCCTTGAGGATGTCGGTGa  <  1:405609/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GCTTCTGTTATTTCCTTTTAAATCAATAAGTTAGCAAATATCTATCACTTCGGTGGTCGTGCATTTTGCAATCAGCGCGGTAAACCTGCATGCAAGATGCAAGTCCGCAAAAGGTTCCCGTCCCCATGAAAAAAGGGCCCTGCGAAGGGCCCTTTCTCTATTGCGTGACGTTCGCTTAGACGCCCGAGGCCTTGGCCGCAGGGACGTCTTTGATCGACAGCTTGATACGGCCGCGGTTGTCCACGTCCAGTACAAGCACTTCAACTTCCTGGCCTTCCTTGAGGATGTCGGTGA  >  NC_002947/5351884‑5352177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑