| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,430,458 | C→T | 8.1% | intergenic (‑92/+69) | PP_4769 ← / ← PP_4770 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,430,458 | 0 | C | T | 8.1% | 160.0 / 3.4 | 58 | intergenic (‑92/+69) | PP_4769/PP_4770 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (26/26); new base T (2/3); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
TGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCAC > NC_002947/5430311‑5430606 | gggCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGt < 2:200796/149‑1 (MQ=255) aCGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGGCACGCCCGCTCCCACGGGTGTAC‑GCGCG‑ATACCTGTGGGAGc < 1:968657/150‑1 (MQ=25) aCGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTAC‑GCGCG‑ATACCTGTGGGAGc > 1:872956/1‑150 (MQ=37) gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTAC‑GCGCG‑ATACCTGTGGGAGCggg < 2:872956/150‑1 (MQ=37) gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCC‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGc < 2:1088042/150‑1 (MQ=255) tagtagAGGTTATGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGt < 2:1222963/150‑1 (MQ=255) tCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAg < 2:533949/150‑1 (MQ=255) cTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTTGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg < 2:22881/150‑1 (MQ=255) cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGgc < 1:278750/150‑1 (MQ=255) aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCa > 1:1067797/1‑150 (MQ=255) aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCa > 1:510328/1‑150 (MQ=255) gAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAggg < 2:339280/139‑1 (MQ=255) gAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAggg > 1:339280/1‑139 (MQ=255) gcgcGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCg > 2:1279596/1‑150 (MQ=255) gcgcGATCTTTTTCACCTGGGAACCTCATTTTTGGCATGGAGTGAAGGAGGGTTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAATGGGCGCAAAGCGCCCCCg > 1:863671/1‑150 (MQ=39) gcgATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGa > 1:1174772/1‑150 (MQ=255) gATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACCGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGaaa < 2:863671/150‑1 (MQ=255) tCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTg > 2:118081/1‑105 (MQ=255) tCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTg < 1:118081/105‑1 (MQ=255) tttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGcc > 2:807508/1‑150 (MQ=255) tttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGcc > 1:575580/1‑150 (MQ=255) tttttCACCTGGGAACCCCATTTTTTTTTTTTTTTTCCGTGGTGCTGCTGCGCAGACCTTCTTTTTCATGCCCGCTCCCACAGGTAT‑C‑GCGCTTACACCCGTGGTATCGTGCGTGCCCGCGAAGGGGCTCAAAGCGCCCCCGGAAATGcc > 2:487742/1‑150 (MQ=255) ttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca < 2:723926/150‑1 (MQ=255) tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCcag < 2:962226/150‑1 (MQ=255) ccTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTa < 2:1067797/150‑1 (MQ=255) gAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCa < 2:510328/150‑1 (MQ=255) gAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCa < 1:501002/150‑1 (MQ=255) aCCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGa < 1:363304/150‑1 (MQ=255) ccTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc < 2:575580/150‑1 (MQ=255) cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGGGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAcc > 1:785656/1‑150 (MQ=255) cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAcc < 2:94759/150‑1 (MQ=255) tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCt > 2:74390/1‑150 (MQ=255) aTTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCAGAAATGCCAGCAGTATGCCAGACCTGt > 2:263291/1‑150 (MQ=255) tttttGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg > 2:438908/1‑105 (MQ=255) tttttGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg < 1:438908/105‑1 (MQ=255) tGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGAc < 2:925874/150‑1 (MQ=255) ggCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACg < 1:807508/150‑1 (MQ=255) gCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGt > 1:1170953/1‑150 (MQ=255) cTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTaa > 1:1002555/1‑150 (MQ=255) cTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTaa > 2:372628/1‑150 (MQ=255) cTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTaa > 2:1085997/1‑150 (MQ=255) tGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAgc > 1:812129/1‑106 (MQ=255) tGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAgc < 2:812129/106‑1 (MQ=255) ccGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc < 1:1279596/150‑1 (MQ=255) ggCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga > 1:693691/1‑150 (MQ=255) tgcGCAGACCTTCGCGGACACGCCCGCTCCCACAGGAGT‑CAGCGC‑TTCGCCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAgaagaa > 2:887702/1‑150 (MQ=38) gcgcAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCGGACGTAAGAATCGTCGTCCGAGAAGAAc < 1:74390/150‑1 (MQ=255) cccacctccTTCGCGGACACGCCCCCTCCCCCAGCTCT‑C‑GCGCGTCCACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAAc < 2:1174652/143‑1 (MQ=255) aCCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCg > 2:748126/1‑150 (MQ=255) cTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAAccagatcgg > 1:1174652/1‑143 (MQ=255) ccGCTCCCACGGGTGTAC‑GCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAGGGGAGCAGGGAGCCCCCGGAGTATTCGGGAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAac > 1:156801/1‑150 (MQ=12) ccGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAac > 2:1029671/1‑150 (MQ=255) ccACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTgtc > 2:553923/1‑38 (MQ=255) ccACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTgtc < 1:553923/40‑3 (MQ=255) gcgcgTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGgc < 1:219329/40‑1 (MQ=255) gcgcgTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGgc > 2:219329/1‑40 (MQ=255) cgcgatacCTGTGGGAGCGGGCGTGTCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGcc < 2:156801/144‑1 (MQ=255) gTACACCAGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCt > 1:644977/1‑150 (MQ=255) ccGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCAc > 1:430279/1‑150 (MQ=255) ccGTGGGAGCGGGCGTACCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCAc > 1:164192/1‑150 (MQ=255) | TGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑C‑GCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCAC > NC_002947/5430311‑5430606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |