Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,503,188	C→T	6.4%	A30A (GCC→GCT)	oprC →	copper receptor OprC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,503,188	0	C	T	6.4%	177.5 / 2.5	62	A30A (GCC→GCT)	oprC	copper receptor OprC
Reads supporting (aligned to +/- strand):  ref base C (29/29);  new base T (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGAC  >  NC_002947/5503046‑5503335                                                                                                                                               |                                                                                                                                                    ttaCGAGGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGcc                                                                                                                                              >  2:961502/3‑150 (MQ=255) gcACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGTTGCTCGCGCCCATGGCCAGGGCCGGCGGcc                                                                                                                                              <  1:247460/150‑1 (MQ=255)   aCGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCt                                                                                                                                            >  1:1179106/1‑150 (MQ=255)           aggaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGa                                                                                                                                    >  1:816643/1‑150 (MQ=255)             gaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTTCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATgggc                                                                                                                                  <  1:1224429/150‑4 (MQ=255)                 cTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCa                                                                                                                              <  1:759700/150‑1 (MQ=255)                  ttCGCGCTAACTCATGACTGATCGACTGGACTCATTATGTAGGGCTTGGGCTCGCTTTTTGCCTTGTCCCTGCGTCGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCAt                                                                                                                             <  1:1249630/150‑1 (MQ=255)                  ttCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCAt                                                                                                                             >  2:73514/1‑150 (MQ=255)                  ttCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCGGTTGTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGTCCTGGCCGCCGGCCCTGGCCATGAAGACCAt                                                                                                                             <  2:494884/150‑1 (MQ=255)                    cgcgCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCAtgt                                                                                                                           <  2:1179106/150‑1 (MQ=255)                        cTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCac                                                                                                                       <  1:73514/150‑1 (MQ=255)                             tCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGc                                                                                                                  >  2:201885/1‑150 (MQ=255)                             tCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGc                                                                                                                  >  2:1176585/1‑150 (MQ=255)                               aTGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCAc                                                                                                                >  2:592824/1‑150 (MQ=255)                                tGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTTCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGa                                                                                                                                 <  1:162151/132‑1 (MQ=255)                                tGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTTCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGa                                                                                                                                 >  2:162151/1‑132 (MQ=255)                                      aTCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGtt                                                                                                         <  2:936345/150‑1 (MQ=255)                                       tCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCAt                                                                                                                             <  1:457662/129‑1 (MQ=255)                                       tCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCAt                                                                                                                             >  2:457662/1‑129 (MQ=255)                                       tCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTg                                                                                                        <  1:201885/150‑1 (MQ=255)                                         gACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAg                                                                                                      <  2:1125284/150‑1 (MQ=255)                                          aCTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTgg                                                                                                                                          >  1:176461/1‑113 (MQ=255)                                          aCTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTgg                                                                                                                                          <  2:176461/113‑1 (MQ=255)                                          aCTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGc                                                                                                     >  2:383411/1‑150 (MQ=255)                                            tGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGccc                                                                                                   >  2:387406/1‑150 (MQ=255)                                               aaTCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCTATGAAGACCATGTGTACGACGCACCCTAGTTGAGCCCgtc                                                                                                >  1:881854/1‑148 (MQ=255)                                                   aTTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCTGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTg                                                                                            >  2:610296/1‑150 (MQ=255)                                                   aTTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCTGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTg                                                                                            >  2:1251794/1‑150 (MQ=255)                                                   aTTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCacgac                                                                                                                    >  2:270543/1‑126 (MQ=255)                                                   aTTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCacgac                                                                                                                    <  1:270543/126‑1 (MQ=255)                                                    ttATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTgg                                                                                                                                          <  2:158296/103‑1 (MQ=255)                                                    ttATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTgg                                                                                                                                          >  1:158296/1‑103 (MQ=255)                                                        gTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGa                                                                                                            <  2:1268613/129‑1 (MQ=255)                                                        gTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGa                                                                                                            >  1:1268613/1‑129 (MQ=255)                                                         tCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACgg                                                                                              <  1:123592/142‑1 (MQ=255)                                                         tCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACgg                                                                                              >  2:123592/1‑142 (MQ=255)                                                              cTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCAATGGCGCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGt                                                                                 <  2:304668/150‑1 (MQ=255)                                                                 cACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCTTGTGCGGCTCGCTCCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGGAGACCAGGTGCACGACGCACCCCAGTTGAGCCCGACGGTGATCGCCGCCGTGGc                                                                              >  1:1172481/1‑150 (MQ=255)                                                                     ccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCg                                                                          >  2:814174/1‑150 (MQ=255)                                                                      cTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGa                                                                         <  1:504495/150‑1 (MQ=255)                                                                        gTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCTGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGt                                                                       <  1:1251794/150‑1 (MQ=255)                                                                        gTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCTGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGt                                                                       <  1:610296/150‑1 (MQ=255)                                                                            ttGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgc                                                                   <  1:387406/150‑1 (MQ=255)                                                                               ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgccgc                                                                <  2:1248820/150‑1 (MQ=255)                                                                                      ccTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGGGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgt                                                         >  1:1175298/1‑150 (MQ=255)                                                                                       cTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgtg                                                        <  1:814174/150‑1 (MQ=255)                                                                                       cTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgtg                                                        <  1:592824/150‑1 (MQ=255)                                                                                          cGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTc                                                     <  2:1175298/150‑1 (MQ=255)                                                                                            tGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCAGGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATCTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTCATtgtgttacc                                                   >  2:436993/1‑145 (MQ=255)                                                                                                aCCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAc                                               >  2:209007/1‑150 (MQ=255)                                                                                                 ccATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                              <  1:1176585/150‑1 (MQ=255)                                                                                                       ccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAg                                        <  1:209007/150‑1 (MQ=255)                                                                                                           ccTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGAccc                                    >  2:1121958/1‑150 (MQ=255)                                                                                                            cTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcg                                   >  2:1130481/1‑150 (MQ=255)                                                                                                                  ggCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGAc                                                            >  2:1092175/1‑119 (MQ=255)                                                                                                                  ggCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGAc                                                            <  1:1092175/119‑1 (MQ=255)                                                                                                                    cTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagcc                           >  1:967189/1‑150 (MQ=255)                                                                                                                     tCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagcca                          <  1:1130831/150‑1 (MQ=255)                                                                                                                            tCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCgg                   >  1:93618/1‑150 (MQ=255)                                                                                                                              gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGcc                 >  1:469126/1‑150 (MQ=255)                                                                                                                                        cccTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGgcg       >  1:516898/1‑150 (MQ=255)                                                                                                                                            ggCCGCCGGCCCTGGGCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGa   <  1:961502/150‑1 (MQ=255)                                                                                                                                             gccgccGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGAGCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGAc  <  2:141535/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    GCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGAC  >  NC_002947/5503046‑5503335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑