Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,567,229 A→T 8.8% L103* (TTG→TAG) ‡ mutL ← DNA mismatch repair protein MutL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,567,2290AT 8.8% 157.3 / 5.9 55L103* (TTG→TAG) ‡mutLDNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base A (24/26);  new base T (3/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTCAGCAGTTTGACGCC  >  NC_002947/5567084‑5567377
                                                                                                                                                 |                                                                                                                                                    
cACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGc                                                                                                                                                  <  2:58167/150‑1 (MQ=255)
 aCTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGcc                                                                                                                                                 >  1:262411/1‑150 (MQ=255)
   ttCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCTTGGCCTc                                                                                                                                               >  1:506518/1‑150 (MQ=255)
   ttCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTc                                                                                                                                               >  1:527684/1‑150 (MQ=255)
      cACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCTTGGCCTCgat                                                                                                                                            <  2:506518/150‑3 (MQ=255)
             gTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCATGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAg                                                                                                                                     <  1:518266/150‑1 (MQ=255)
              tACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGc                                                                                                                                    <  1:743325/150‑1 (MQ=255)
                 cgaccgGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCa                                                                                                                                 >  2:839458/1‑150 (MQ=255)
                    ccgGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTGCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGc                                                                                                                              >  1:644833/1‑150 (MQ=255)
                        gTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCGCGCCACGGAAGCCCAGGCTCaa                                                                                                                          >  2:1271811/1‑150 (MQ=255)
                           cgccgcCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATAc                                                                                                                       <  1:1062901/150‑1 (MQ=255)
                            gccgccGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCTTGGCCTCga                                                                                                                                             >  1:87094/1‑126 (MQ=255)
                            gccgccGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCTTGGCCTCga                                                                                                                                             <  2:87094/127‑2 (MQ=255)
                            gccgccGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGaa                                                                                                                                      <  1:735673/134‑1 (MQ=255)
                            gccgccGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGaa                                                                                                                                      >  2:735673/1‑134 (MQ=255)
                                    cTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAg                                                                                                              <  1:1025502/150‑1 (MQ=255)
                                        aCCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCt                                                                                                          <  1:1059437/150‑1 (MQ=255)
                                          ccGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTc                                                                                                        <  1:643940/150‑1 (MQ=255)
                                                          cgGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAg                                                                                                              <  2:1040381/128‑1 (MQ=255)
                                                          cgGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAg                                                                                                              >  1:1040381/1‑128 (MQ=255)
                                                              cTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCtgg                                                                                    >  1:1152134/1‑150 (MQ=255)
                                                               ttCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCtggt                                                                                   >  2:484935/1‑150 (MQ=255)
                                                                tCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTTATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCtggtg                                                                                  <  1:642168/150‑1 (MQ=255)
                                                                tCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCtggtg                                                                                  <  1:109628/150‑1 (MQ=255)
                                                                   gTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTc                                                                                                        <  2:979930/125‑1 (MQ=255)
                                                                   gTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTc                                                                                                        >  1:979930/1‑125 (MQ=255)
                                                                      tCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCgtgt                                                                            >  1:61525/1‑150 (MQ=255)
                                                                      tCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCgtgt                                                                            <  2:61525/150‑1 (MQ=255)
                                                                       cTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTTGTTTCGTGTc                                                                           <  1:437477/150‑1 (MQ=255)
                                                                        tACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGc                                                                                >  1:663047/1‑144 (MQ=255)
                                                                        tACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGc                                                                                <  2:663047/144‑1 (MQ=255)
                                                                        tACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCg                                                                          >  1:133285/1‑150 (MQ=255)
                                                                        tACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCg                                                                          >  1:442945/1‑150 (MQ=255)
                                                                              ccAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAg                                                                    >  1:354188/1‑150 (MQ=255)
                                                                              ccAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGATCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAg                                                                    >  1:1039947/1‑150 (MQ=255)
                                                                               cAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAgg                                                                   <  2:527684/150‑1 (MQ=255)
                                                                                    cTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCAc                                                                                                                                          <  2:409131/74‑1 (MQ=255)
                                                                                    cTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCAc                                                                                                                                          >  1:409131/1‑74 (MQ=255)
                                                                                             actggcGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGgtc                                                     <  1:484935/150‑1 (MQ=255)
                                                                                                 gcGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGtcgtcg                                                 <  2:1039947/150‑1 (MQ=255)
                                                                                                 gcGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTAGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGtcgtcg                                                 >  1:1123364/1‑150 (MQ=255)
                                                                                                   ggTACGCGCGGTCAGGGTCAGCCGCGCCACCGACCTGATCCAGGCCAAGGCCTCGCCACGGACGCCCACGCTCAATACCCCTTCCAGGTCTTCCAGCTCGCGGCTCTTGCTCGCGGCGTGTCGAGCCCCCGCCAGCGGCAGGccgtcgtc                                               >  2:51887/1‑148 (MQ=255)
                                                                                                        gcgAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGaaa                                          >  1:297515/1‑150 (MQ=255)
                                                                                                               cagggtcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCt                                   <  2:354188/150‑1 (MQ=255)
                                                                                                                 gggtcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGc                                 <  1:889456/150‑1 (MQ=255)
                                                                                                                 gggtcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGc                                 >  1:381623/1‑150 (MQ=255)
                                                                                                                 ggggcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGc                                 <  2:262411/150‑1 (MQ=255)
                                                                                                                         cgcgcCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATcc                         <  1:839458/150‑1 (MQ=255)
                                                                                                                                 cGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCAc                    <  1:896411/147‑1 (MQ=255)
                                                                                                                                 cGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCAc                    >  2:896411/1‑147 (MQ=255)
                                                                                                                                  gAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGTCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTc                <  2:922795/150‑1 (MQ=255)
                                                                                                                                     cgtggcGAGGCCTTGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTcagc             >  1:840109/6‑150 (MQ=255)
                                                                                                                                      tGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTc                                                                           >  2:759484/1‑87 (MQ=255)
                                                                                                                                      tGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTc                                                                           <  1:759484/87‑1 (MQ=255)
                                                                                                                                                cAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTCAGCAGTTTGAcgcc  >  2:662496/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTCAGCAGTTTGACGCC  >  NC_002947/5567084‑5567377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑