Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,577,872 C→T 8.4% intergenic (+7/‑38) PP_4906 → / → rhdA hypothetical protein/thiosulfate sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,577,8720CT 8.4% 125.8 / 3.2 48intergenic (+7/‑38)PP_4906/rhdAhypothetical protein/thiosulfate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base T (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

GCAGCCAACGACCAGGTGCTGATGCTGCTGGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTGATCCTGGTCGACCTGAGCAGCGCCAACCGCTATGT  >  NC_002947/5577725‑5578019
                                                                                                                                                   |                                                                                                                                                   
gCAGCCAACGACCAGGTGCTGATGCTGGTGGTGGCCGACCAGGGCTGCCGGACTGTGGCCGATATCAGCGTGCAAGCCTTCGGTAAGACCGCGAAATACATCGAGCTGGCCTTGTCGGTGTTTACAAACCGGAAGAGTTGAGCCTTgcgc                                                                                                                                                   >  2:104842/1‑150 (MQ=255)
  aGCCAACGACCAGGTGCTGATGCTGCTGGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTa                                                                                                                                                 >  2:546308/1‑150 (MQ=255)
  aGCCAACGACCAGGTGCTGATGCTGCTGGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTa                                                                                                                                                 >  1:919817/1‑150 (MQ=255)
  aGCCAACGACCAGGTGCTGATGCTGCTGCTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAACCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTTTTTACCAACCGCAAGGGTTGAGCCTTGCGCTa                                                                                                                                                 >  2:1166812/1‑150 (MQ=255)
    ccAACGACCAGGTGCTGATGCTGCTGGTTGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACa                                                                                                                                               >  2:778495/1‑150 (MQ=255)
      aaCGACAAGGTGCTGATGCTGCTGGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAAt                                                                                                                                             >  1:1276884/1‑150 (MQ=255)
         gACCAGGTGCTGATGCTGCTGGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGc                                                                                                                                          <  2:15142/150‑1 (MQ=255)
            cAGGTGCTGATGCTGCTGGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGcccc                                                                                                                                       <  2:1144816/150‑1 (MQ=255)
                    gATGCTGCTGGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGAAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGAGCTACACTCGGCCCTCTTGCca                                                                                                                               <  2:282717/150‑1 (MQ=255)
                         tgctgGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGcc                                                                                                                                         <  2:534864/135‑1 (MQ=255)
                         tgctgGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGcc                                                                                                                                         >  1:534864/1‑135 (MQ=255)
                                 gCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCCCCATGGAGACCGt                                                                                                                  >  1:268197/1‑150 (MQ=255)
                                       cAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGa                                                                                                            <  1:778495/150‑1 (MQ=255)
                                         gggCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGAcc                                                                                                          <  2:919817/150‑1 (MQ=255)
                                            cTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGTAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGAc                                                                                                       <  1:440910/150‑1 (MQ=255)
                                            cTGCCGCCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGGCGAACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGAc                                                                                                       <  1:977855/150‑1 (MQ=255)
                                                                  aGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGa                                                                                 >  1:1252112/1‑150 (MQ=255)
                                                                  aGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGa                                                                                 >  2:1128152/1‑150 (MQ=255)
                                                                   gCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGAt                                                                                >  2:558171/1‑150 (MQ=255)
                                                                        cAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAg                                                                           <  1:546308/150‑1 (MQ=255)
                                                                        cAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAg                                                                           >  1:696289/1‑150 (MQ=255)
                                                                         aaGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCttt                                                                                                                                  >  1:291076/1‑94 (MQ=255)
                                                                         aaGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCttt                                                                                                                                  <  2:291076/94‑1 (MQ=255)
                                                                           gCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTgg                                                                                    >  1:15628/1‑138 (MQ=255)
                                                                           gCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTgg                                                                                    <  2:15628/138‑1 (MQ=255)
                                                                             cTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCa                                                                      <  2:1252112/150‑1 (MQ=255)
                                                                                 ggCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGTGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGAcc                                                                  >  1:1206051/1‑150 (MQ=255)
                                                                                     aGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACctgct                                                              <  1:1128152/150‑1 (MQ=255)
                                                                                     aGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACctgct                                                              <  2:268197/150‑1 (MQ=255)
                                                                                          gcgcAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCAc                                                         >  2:808414/1‑150 (MQ=255)
                                                                                            gcAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGAt                                                                                                              <  1:236997/95‑1 (MQ=255)
                                                                                            gcAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGAt                                                                                                              >  2:236997/1‑95 (MQ=255)
                                                                                            gcAATGCAGCGAGCGCGCCTTGTCGGTGTTTACCAACCACAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGc                                                       <  2:125281/150‑1 (MQ=255)
                                                                                              aaTGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGTTCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCt                                                     <  2:1276884/150‑1 (MQ=255)
                                                                                               aTGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGTGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCt                                                     >  2:593314/1‑149 (MQ=255)
                                                                                               aTGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGTGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCt                                                     <  1:593314/149‑1 (MQ=255)
                                                                                                           ggCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCaa                                        <  1:220001/150‑1 (MQ=255)
                                                                                                            gCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCtgctgc                                                            >  2:39303/1‑129 (MQ=255)
                                                                                                            gCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCtgctgc                                                            <  1:39303/129‑1 (MQ=255)
                                                                                                              cTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTg                                     >  1:889916/1‑150 (MQ=255)
                                                                                                                     gtgtTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGGTGACCGACTTTTCCGCCCTGCCCCTGGCGATCGACGCTGCAGACCTGCTGCCACGCCTGGATTCGCGGCAACTGATCCTgg                              >  1:1053716/1‑150 (MQ=255)
                                                                                                                        tttACCAACCGCAAGGGTTGAGCCTTGTGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTGATCCTGGTCg                           <  2:1206051/150‑1 (MQ=255)
                                                                                                                          tACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGATGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTGATCCTGGTCGAc                         >  2:782305/1‑150 (MQ=255)
                                                                                                                              aaCCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTGATCCTGGTCGACCTGa                     <  2:889916/150‑1 (MQ=255)
                                                                                                                               aCCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTGATCCTGGTCGACCTGag                    <  1:782305/150‑1 (MQ=255)
                                                                                                                                 cgaaaGGGTTCAGCCTGGCGGTACAATCCCTCCTC‑TTCCGCACGGGAGACCTTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTGATCCTGGTCGACCTGagcag                 >  2:1013125/4‑150 (MQ=255)
                                                                                                                                 cGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCGCCATCCTGATTCTCCGCAACTGATCCTGGTCGACCTGagca                  >  2:304007/1‑150 (MQ=255)
                                                                                                                                         ttGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTGATCCTGGTCGACCTGAGCAGCGCCAAc          <  1:808414/150‑1 (MQ=255)
                                                                                                                                                 tGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTGATCCTGGTCGACCTGAGCAGCGCCAACCGCTATGt  >  1:1269523/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCAGCCAACGACCAGGTGCTGATGCTGCTGGTGGCCGACCAGGGCTGCCGGCCTTTGGCCGATATCAGCGTGCAAGCCTTCGGCAAGACCGCGCAATGCATCGAGCGGGCCTTGTCGGTGTTTACCAACCGCAAGGGTTGAGCCTTGCGCTACAATCGCCCCTCTTTCCACACTGGAGACCGTGGATGACCGACTTTTCCGGCCTGCCCCTGGTGATCGAAGCTGCAGACCTGCTGCCACGCCTGGATTCGCCGCAACTGATCCTGGTCGACCTGAGCAGCGCCAACCGCTATGT  >  NC_002947/5577725‑5578019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑