| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,587,812 | G→T | 7.1% | Q463K (CAG→AAG) | parE ← | DNA topoisomerase IV subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,587,812 | 0 | G | T | 7.1% | 178.4 / 2.5 | 56 | Q463K (CAG→AAG) | parE | DNA topoisomerase IV subunit B |
| Reads supporting (aligned to +/- strand): ref base G (32/20); new base T (2/2); total (34/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
AACGAACAGCGCACACAGCAATGTGGCGATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATC > NC_002947/5587666‑5587960 | aacgaacAGCGCACACAGCAATGTGGCGATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCctggct > 1:6975/1‑150 (MQ=255) gaacAGCGCACACAGCAATGTGGCGATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCtggctggc > 1:264116/1‑150 (MQ=255) gcgcACACAGCAATGTGGCGATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGa > 2:506819/1‑150 (MQ=255) acaGCAATGTGGCGATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCCAGCCAGGaagtgca < 1:1028017/150‑7 (MQ=255) tgtgGCGATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTc > 2:1095066/1‑150 (MQ=255) gCGATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACt < 2:264116/150‑1 (MQ=255) gATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCctg > 2:631269/1‑120 (MQ=255) gATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCctg < 1:631269/120‑1 (MQ=255) gCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAgg > 1:1138523/1‑150 (MQ=255) cAGACCATCGGAGTCAGCGTCGGCGAGGATGGAGACATTGTACTGGTGTGGACTG‑GCTAGGTCCCTGGTTGCTGGAGCGACGCCACTGGCCACGGCAATGTTGTGCACTTCCTTTCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGgt > 1:348708/1‑150 (MQ=255) cAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATGTTCACATTACCTAG‑TAACGCAAGGTCGTTTGCACGCTGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTTGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGgt > 1:348791/1‑150 (MQ=255) gACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGgtgt < 1:841691/150‑1 (MQ=255) cATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGa < 1:89451/150‑1 (MQ=255) aTCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAg > 1:614492/1‑150 (MQ=255) gCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCcg > 1:1118421/1‑150 (MQ=255) gaagATGGAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCgg > 2:1216343/4‑150 (MQ=255) gCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGAt > 1:1070111/1‑150 (MQ=255) aTCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGcc < 1:506819/150‑1 (MQ=255) tCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCt < 2:6975/150‑1 (MQ=255) cATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATATTGCCGAGCCCGTTCAGGATGGCCTGGAAtt > 1:707138/1‑150 (MQ=255) aTAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTcc > 1:413456/1‑103 (MQ=255) aTAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTcc < 2:413456/103‑1 (MQ=255) aTAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTc < 1:72499/150‑1 (MQ=255) aTAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTc < 2:1118421/150‑1 (MQ=255) gcAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGt < 2:1138523/150‑1 (MQ=255) tGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTTGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCcgc < 2:348708/150‑1 (MQ=255) tGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTTGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCcgc < 2:348791/150‑1 (MQ=255) cAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCt > 1:721938/1‑150 (MQ=255) aaGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCa < 2:174234/65‑1 (MQ=255) aaGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCa > 1:174234/1‑65 (MQ=255) tCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCGGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTg > 2:685925/1‑150 (MQ=255) tCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGCATTCCTTGTAGCGCGCCTGCGTg > 2:1036291/1‑150 (MQ=255) gTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGTCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGcc < 1:1048631/150‑1 (MQ=255) gTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGcc > 2:1142979/1‑150 (MQ=255) cACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGCCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAac > 2:821267/1‑150 (MQ=255) aCCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAacc > 1:960481/1‑150 (MQ=255) aCCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAacc > 1:1239198/1‑150 (MQ=255) ccGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGc > 2:949077/1‑104 (MQ=255) ccGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGc < 1:949077/104‑1 (MQ=255) ccGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGaa < 1:1272763/118‑1 (MQ=255) ccGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGaa > 2:1272763/1‑118 (MQ=255) ccGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAaccac > 2:510087/1‑150 (MQ=255) tCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGct < 1:685925/150‑2 (MQ=255) tCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGcc > 2:442484/1‑150 (MQ=255) cGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCg > 2:1049031/1‑150 (MQ=255) aCGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAg > 1:1072242/1‑150 (MQ=255) aaTGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTcc > 2:297683/1‑115 (MQ=255) aaTGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTcc < 1:297683/115‑1 (MQ=255) gCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTcg > 2:261923/1‑150 (MQ=255) cACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGcc > 1:697972/1‑124 (MQ=255) cACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGcc < 2:697972/124‑1 (MQ=255) gCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAgg < 1:1216343/150‑1 (MQ=255) tgttgtGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGCGTCACCCTCGACGAGGcaca > 2:800857/1‑150 (MQ=255) ttgtGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGCCCGAGTCACCCTCGACGAGGaacaac > 2:293342/1‑150 (MQ=255) gCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCcgc > 2:400323/1‑98 (MQ=255) gCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCcgc < 1:400323/98‑1 (MQ=255) agtCCTGGCCAGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCAc > 2:1128963/3‑150 (MQ=255) agtCCTGGCCAGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACACTCGACGAGGAACAACTCGGCAc > 1:105238/3‑150 (MQ=255) ttCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCt < 1:1234429/53‑1 (MQ=255) ttCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCt > 2:1234429/1‑53 (MQ=255) ctggctggCCAGGACTTCGCCCCCGTCCACTTCCCAGGGGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCAt > 1:1134634/1‑150 (MQ=255) tggctggcCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATc < 2:1100962/150‑1 (MQ=255) | AACGAACAGCGCACACAGCAATGTGGCGATGTGCAGACCATCGGAGTCAGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAG‑CTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATC > NC_002947/5587666‑5587960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |