Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,844,171 C→T 8.3% A246V (GCC→GTC)  PP_5122 → GMC family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,844,1710CT 8.3% 165.9 / 4.9 62A246V (GCC→GTC) PP_5122GMC family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (30/26);  new base T (3/2);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

CTGCCCGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAACCTTCCT  >  NC_002947/5844022‑5844315
                                                                                                                                                     |                                                                                                                                                
cTGCCCGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGc                                                                                                                                                  <  1:117512/150‑1 (MQ=255)
      ggTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAggg                                                                                                                                            <  1:738612/150‑1 (MQ=255)
      ggTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCCCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAggg                                                                                                                                            <  2:1240303/150‑1 (MQ=255)
         gAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCGCAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGGCGTCCAGGGcct                                                                                                                                         <  2:1128323/150‑3 (MQ=255)
               cAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACAccc                                                                                                                                   >  2:318134/1‑150 (MQ=255)
                 aaCAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGGCGTCCAGGGcctg                                                                                                                                        >  1:94229/1‑140 (MQ=255)
                 aaCAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGGCGTCCAGGGcctg                                                                                                                                        <  2:94229/143‑4 (MQ=255)
                    aagcGATGCTGGTGACCCGTATACCTGCCACCCTCGTGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCg                                                                                                                              <  2:563075/147‑1 (MQ=255)
                    aaTCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCg                                                                                                                              <  1:314307/150‑1 (MQ=255)
                     aTCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCgg                                                                                                                             >  1:48444/1‑150 (MQ=255)
                      tCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCggc                                                                                                                            >  1:558910/1‑150 (MQ=255)
                      tCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCggc                                                                                                                            <  1:515714/150‑1 (MQ=255)
                       cGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCggca                                                                                                                           <  2:61553/150‑1 (MQ=255)
                         aTGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGc                                                                                                                                                  >  2:361791/1‑125 (MQ=255)
                         aTGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGc                                                                                                                                                  <  1:361791/125‑1 (MQ=255)
                            cTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGgcaggcag                                                                                                                      >  2:1212104/1‑150 (MQ=255)
                                tGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCa                                                                                                                  >  1:302198/1‑150 (MQ=255)
                                 gACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAg                                                                                                                 >  2:209771/1‑150 (MQ=255)
                                     cGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTg                                                                                                             >  2:100174/1‑150 (MQ=255)
                                       tataCCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCa                                                                                                           <  2:1186496/150‑1 (MQ=255)
                                            cTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCAc                                                                                                      >  2:539766/1‑150 (MQ=255)
                                             tGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACg                                                                                                     >  2:946614/1‑150 (MQ=255)
                                                cACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCa                                                                                                  >  1:135773/1‑150 (MQ=255)
                                                  cccTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGCCGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCAtt                                                                                                >  1:1226200/1‑150 (MQ=255)
                                                   ccTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGGGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTAAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCACGCACGCCAtga                                                                                               >  1:967163/1‑148 (MQ=255)
                                                   ccTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTa                                                                                               >  2:521293/1‑150 (MQ=255)
                                                   ccTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTa                                                                                               >  2:903926/1‑150 (MQ=255)
                                                     tCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAcc                                                                                             >  1:1219989/1‑150 (MQ=255)
                                                     tCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAc                                                                                              >  2:630942/1‑149 (MQ=255)
                                                     tCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAc                                                                                              <  1:630942/149‑1 (MQ=255)
                                                         gCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAcctcct                                                                                         <  1:903926/150‑1 (MQ=255)
                                                            aGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGc                                                                                      <  2:558910/150‑1 (MQ=255)
                                                                 gcgAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTg                                                                                 >  2:1016231/1‑150 (MQ=255)
                                                                    aaCTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCACTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCg                                                                              <  1:1152355/150‑1 (MQ=255)
                                                                       tgctgTATCTAGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAACTCCTGGCCGGTGGCGGTa                                                                           >  2:473063/1‑150 (MQ=255)
                                                                         ctgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGTACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGGCGGGAGCGGAAta                                                                         >  1:221263/1‑149 (MQ=255)
                                                                         ctgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTgg                                                                                       <  2:264221/136‑1 (MQ=255)
                                                                         ctgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTgg                                                                                       >  1:264221/1‑136 (MQ=255)
                                                                         ctgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATc                                                                         <  2:221460/150‑1 (MQ=255)
                                                                          tgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGCCCCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACCCCCTTCCCTCCTGGCCCGTCGCGGTATCa                                                                        >  2:161996/1‑150 (MQ=255)
                                                                           gTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCaa                                                                       >  2:242851/1‑150 (MQ=255)
                                                                              tCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAg                                                                    <  1:318134/150‑1 (MQ=255)
                                                                                 ggCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGccc                                                                 <  1:1016231/150‑1 (MQ=255)
                                                                                            aGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCTCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCctgctgc                                                      <  1:1126145/150‑1 (MQ=255)
                                                                                                cTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTg                                                                                 <  2:191114/119‑1 (MQ=255)
                                                                                                cTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTg                                                                                 >  1:191114/1‑119 (MQ=255)
                                                                                                cTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTgcg                                                  >  1:4662/1‑150 (MQ=255)
                                                                                                   cGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGctc                                               >  2:840681/1‑150 (MQ=255)
                                                                                                           aCGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTgcgc                                                 >  1:629111/1‑140 (MQ=255)
                                                                                                           aCGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTgcgc                                                 <  2:629111/140‑1 (MQ=255)
                                                                                                                     cATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCa                             <  2:4662/150‑1 (MQ=255)
                                                                                                                      aTTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCAt                            <  1:209771/150‑1 (MQ=255)
                                                                                                                      aTTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCAt                            >  2:168815/1‑150 (MQ=255)
                                                                                                                      aTTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCAt                            <  2:96584/150‑1 (MQ=255)
                                                                                                                             gCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGcc                     >  2:1162459/1‑150 (MQ=255)
                                                                                                                                tGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTgg                  <  1:168815/150‑1 (MQ=255)
                                                                                                                                     tGCCAGGCCCTGTACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCTGAGTGCAGGCACACGATTACCGCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAg             <  1:473063/150‑1 (MQ=255)
                                                                                                                                        tatgCCCTGGGCGTCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGa          >  2:523865/4‑150 (MQ=255)
                                                                                                                                         aGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATgg                          <  2:1140499/133‑1 (MQ=255)
                                                                                                                                         aGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATgg                          >  1:1140499/1‑133 (MQ=255)
                                                                                                                                          tgctccggACGGCCAGGGCAGGCACCCTCCCGGCAGATAGCTTAGCGTGCAGGCACGCCATTGCCTCCTGGCCGGTGGCGGTATCCACAGCCCCGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCCGGGCAGCCGAAc        <  2:221263/144‑1 (MQ=255)
                                                                                                                                            cccTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAACCt      >  1:722096/1‑150 (MQ=255)
                                                                                                                                              cTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAACCTTc    <  1:946614/150‑1 (MQ=255)
                                                                                                                                               tGGGCGTCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCgg                                                                             >  1:246489/1‑76 (MQ=255)
                                                                                                                                                ggACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAACCTTCCt  <  2:1163560/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CTGCCCGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACATGCCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAACCTTCCT  >  NC_002947/5844022‑5844315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑