| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,846,019 | G→A | 8.5% | intergenic (+35/+56) | yfhL → / ← mutM | 4Fe‑4S cluster‑containing protein/formamidopyrimidine‑DNA glycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,846,019 | 0 | G | A | 8.5% | 156.2 / 5.5 | 59 | intergenic (+35/+56) | yfhL/mutM | 4Fe‑4S cluster‑containing protein/formamidopyrimidine‑DNA glycosylase |
| Reads supporting (aligned to +/- strand): ref base G (27/27); new base A (3/2); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01 | |||||||||||
AGCCGCAGTGCCAGCAGGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGC > NC_002947/5845872‑5846166 | aGCCGCAGTGCCAGCAGGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCggg < 2:444614/150‑1 (MQ=255) cAGTGCCAGCAGGTCTGCCCGGTCGACTGCATACCGCGGTATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCAGTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGc < 2:743141/150‑1 (MQ=255) aGTGCCAGCAGGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGcc < 2:672290/150‑1 (MQ=255) tGCCAGCAGGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTg > 2:835642/1‑150 (MQ=255) ccAGCAGGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCt < 1:457253/150‑1 (MQ=255) ggTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGCTACCGAAGAACATCTGATGGCCAAGTCTATCTTGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCcaca < 1:926729/150‑1 (MQ=255) gTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCcaca > 1:1204928/1‑149 (MQ=255) gTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCcaca < 2:1204928/149‑1 (MQ=255) tCTGCCGGGTCGACTGCATCCCGCTGGATGAAGAGAATCCAGATTCCGAATAACAGCTGATGGCCAAGTATAGTAATTTTACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGGGAGCAGGCATGCCCGCTCTCACAgg < 1:926445/150‑2 (MQ=255) gCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTa > 1:109695/1‑150 (MQ=255) gCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCAAGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTa < 1:1232285/150‑1 (MQ=255) gTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGttt < 2:200220/150‑1 (MQ=255) tCGACTGCATCCCGCTGGATGAAGCGCATCCGGACACCGAAGAACAGCTGATGGCCAAGTATGGCCGGATGACTGGCAAGGGCTGATCATTGCCCTTCCCTTCCCGGTCCCTGTGAGAGCGGGCCTGCCTGCTCCCAGCGTTTAGATTTc > 2:101148/1‑150 (MQ=255) aCTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCt > 2:1214770/1‑150 (MQ=255) gCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGtt > 1:1128637/1‑150 (MQ=255) cTGGATGAAGCGCATCAGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGtcc < 2:566284/150‑3 (MQ=255) gATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCAAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTGCCCTGCACGGTGCCTGTGAGAGCGGGCATGCCGGCTCCGTCAGTTTAGGTTTCGCTTCAACAGGTTCGCa < 2:292100/150‑1 (MQ=255) aGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGaa < 2:527885/150‑1 (MQ=255) gcgcATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAAc > 2:849815/1‑150 (MQ=255) cATCCCGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGTTCATTGCCCTTCCCTTCACCGTCCCTGTGAGGGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGtt < 1:101148/150‑1 (MQ=255) aTCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCa < 1:1117740/140‑1 (MQ=255) aTCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCa > 2:1117740/1‑140 (MQ=255) aTCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGAGGGCGTGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGttt > 2:676003/1‑150 (MQ=255) ggAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATc > 1:1147692/1‑150 (MQ=255) ggAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTACATCTGATGGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTCCAAACGTTTCGCATTGAACggggcttc > 1:921680/1‑143 (MQ=255) ccGAAGAACAGCTGATGGCCAAGTATCGCCGGATCCCTGGCAAGGGCTGATCATTGCCCTTTCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTgg < 1:650996/150‑1 (MQ=255) cAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTa < 1:801661/100‑1 (MQ=255) cAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTa > 2:801661/1‑100 (MQ=255) aGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCggg < 2:1147692/150‑1 (MQ=255) aGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCggg < 1:835642/150‑1 (MQ=255) tGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAAAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAg > 2:32540/1‑150 (MQ=255) gATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGt < 2:695836/150‑1 (MQ=255) tGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTac > 1:155128/1‑150 (MQ=255) aGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTc < 2:793068/150‑1 (MQ=255) gTATCGCCTGATCACTCGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAATTTAGGTTTCGCCTCAACAGGTTCGCAGCGAACGTTTCATTGTTACCAGCGCGGGCAGTACAGCCTCg > 2:28716/1‑150 (MQ=255) tCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGccc > 1:1285714/1‑150 (MQ=255) cTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCCTCGTTgg > 2:140452/1‑114 (MQ=255) cTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTgg < 1:140452/114‑1 (MQ=255) tGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGtt > 2:11131/1‑111 (MQ=255) tGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGtt < 1:11131/111‑1 (MQ=255) cAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCa < 2:1086473/150‑1 (MQ=255) aaGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGtt > 2:447693/1‑107 (MQ=255) aaGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGtt < 1:447693/107‑1 (MQ=255) gCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTcg > 1:119976/1‑150 (MQ=255) aTTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACt > 1:520367/1‑150 (MQ=255) cccTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATc > 1:1225895/1‑89 (MQ=255) cccTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATc < 2:1225895/89‑1 (MQ=255) cccTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGc > 1:941924/1‑150 (MQ=255) ctaaacTGTGGGAGCAGGCATGCCCGCTCTCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATAc < 1:1045890/141‑1 (MQ=39) ctaaacTGTGGGAGCAGGCATGCCCGCTCTCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATAc > 2:1045890/6‑146 (MQ=39) ccTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACg > 2:1012133/1‑150 (MQ=255) acTGTGGGAGCAGGCATGCCCGCTCTCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACg > 2:906267/2‑150 (MQ=255) cTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACgg < 1:1214770/150‑1 (MQ=255) tgtgAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGc > 2:446251/1‑105 (MQ=255) tgtgAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGc < 1:446251/105‑1 (MQ=255) tgtgAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATCACTTGCACGGc > 1:379118/1‑150 (MQ=255) gtgGGAGCAGGCATGCCCGCTCTCACAGTTTAGGTTTCGCTTCAACAGGGTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCt > 2:501855/1‑150 (MQ=255) gagaGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGc < 2:379118/150‑1 (MQ=255) gagaGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGc > 2:256541/1‑150 (MQ=255) gCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGc < 1:256541/150‑1 (MQ=255) | AGCCGCAGTGCCAGCAGGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATCACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCCCGC > NC_002947/5845872‑5846166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |