| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,032,849 | G→C | 6.8% | intergenic (+57/+79) | dppA‑IV → / ← PP_5284 | dipeptide transport protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,032,849 | 0 | G | C | 6.8% | 157.9 / 2.6 | 56 | intergenic (+57/+79) | dppA‑IV/PP_5284 | dipeptide transport protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (26/25); new base C (2/2); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
CGGCGACCTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAATGATGATGTGATCCA > NC_002947/6032704‑6032995 | cggcgACCTTGCTGCGCCAACGCGTCGCCGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑Gt < 2:599371/150‑1 (MQ=255) gctgcGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTAcc > 1:1214215/1‑150 (MQ=255) cAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTATCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAg > 2:245161/1‑150 (MQ=255) cAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGTCA‑‑ACTGCCA‑GGCTGGCGCTACCCCTGTAg < 2:320803/150‑1 (MQ=25) cAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAg > 1:240818/1‑150 (MQ=255) ggCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAg < 1:1127834/150‑1 (MQ=255) gcGAGGGGTACCAGGTGAGCCCGTGCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAACCCCGCGCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCggg < 2:437119/149‑1 (MQ=255) gTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTAccc < 2:240818/150‑1 (MQ=255) gTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCGTGCCA‑GT‑TGACGCTACCCCTGTAGTAGCGGGTTTACCCGCGagag > 1:637356/1‑150 (MQ=255) gAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAggg < 1:749297/150‑1 (MQ=255) ggCCCGTTGGGGCGGCTGGATTTCAGCAAGGGGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAACCCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGc < 2:1214215/149‑1 (MQ=255) cccGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGccc > 2:161767/1‑150 (MQ=255) cccGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGccc > 1:263459/1‑150 (MQ=255) ccGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTAccc < 1:134110/137‑1 (MQ=255) ccGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTAccc > 2:134110/1‑137 (MQ=255) gggCGGCTGGAGTTCAGCAAGGTGACGGTGACCAAGTAAGACTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGa < 1:53155/150‑1 (MQ=255) gcTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCta < 1:1219173/150‑1 (MQ=255) atctaTTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGTACTGACCCtat < 2:1254799/146‑1 (MQ=255) aTTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGGGCCA‑GACTGGCA‑GT‑TGCCCGTACCCGTGTAGTGGCTTGTTCACCCGCGAAAGGGCCCGTACTGACCCTATAAgg < 1:161767/150‑1 (MQ=37) tttCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGc > 2:23346/1‑150 (MQ=255) tttCAGCAAGGTGACGGTGACAAAGTCAGCCCATACCGGCCTCTTCGCGGGTAAACTCGCTCCTACAGGGGTAGAGCGATGGAATACA‑GG‑CG‑CGGGGCCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCATGTACTGACCCTATAAgat > 1:1254799/1‑148 (MQ=255) aGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCtctc < 2:263459/150‑1 (MQ=255) gacggtgacCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTcc > 1:914009/1‑150 (MQ=255) gacggtgacCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCCCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTcc > 2:749053/1‑150 (MQ=255) tgacCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAg > 1:690619/1‑92 (MQ=255) tgacCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAg < 2:690619/92‑1 (MQ=255) tgacCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTAGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAg > 1:1198575/1‑150 (MQ=255) gacGAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGa < 2:1019568/150‑1 (MQ=255) gCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCa > 1:922766/1‑150 (MQ=255) ccTGTACCGGCCTCTTCGCGGGTCAACCCGCTCCTACAGGTGTAGCGCCC‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGTAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCCGCCCCCAAtgctc < 2:637356/150‑5 (MQ=38) gTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTAGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCa < 2:1198575/150‑1 (MQ=255) tACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCAt < 2:174307/150‑1 (MQ=255) gCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCa < 2:1038193/117‑1 (MQ=255) gCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCg > 1:739730/1‑150 (MQ=255) gCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCCCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCa > 1:1038193/1‑117 (MQ=255) tctTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGAc < 2:558083/149‑1 (MQ=255) tctTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGAc > 1:558083/1‑149 (MQ=255) cccGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACgg > 2:1090516/1‑147 (MQ=255) cccGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACgg < 1:1090516/147‑1 (MQ=255) cccGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTc > 2:756403/1‑150 (MQ=255) cccGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTc < 2:922766/150‑1 (MQ=255) ccGCTCCTACAGGGGTAGCGTCA‑‑ACTGCCAGGC‑TGGCGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCa < 2:212150/110‑1 (MQ=14) ccGCTCCTACAGGGGTAGCGTCA‑‑ACTGCCAGGC‑TGGCGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCa > 1:212150/1‑110 (MQ=14) ccGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTcc > 2:396754/1‑150 (MQ=255) gCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTcccc > 2:1029153/1‑150 (MQ=255) tACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCa < 2:122118/92‑1 (MQ=255) tACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCa > 1:122118/1‑92 (MQ=255) cAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCggg > 1:733900/1‑48 (MQ=255) cAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCggg < 2:733900/48‑1 (MQ=255) gggTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAatg > 1:919555/1‑150 (MQ=255) ggTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAatga < 1:396754/150‑1 (MQ=255) gTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAatgat > 1:1026381/1‑150 (MQ=255) tAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAatgatg > 1:990355/1‑150 (MQ=255) ccA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAATGATGAtgtg > 1:1216024/1‑150 (MQ=255) cgggCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAATGATGATGTGATCCa < 1:245161/148‑1 (MQ=255) tGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGAcc > 2:549612/1‑88 (MQ=255) tGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGAcc < 1:549612/88‑1 (MQ=255) | CGGCGACCTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCA‑GCCTGGCA‑GT‑TGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAATGATGATGTGATCCA > NC_002947/6032704‑6032995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |