Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,129,229 A→T 7.8% intergenic (+35/+32) PP_5376 → / ← PP_5377 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,129,2290AT 7.8% 146.4 / 4.9 51intergenic (+35/+32)PP_5376/PP_5377hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/22);  new base T (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATA  >  NC_002947/6129085‑6129375
                                                                                                                                                |                                                                                                                                                  
ggggAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCCCTGAATGCTGGCCAAACGAAAACGGCGCCTTAAAAgc                                                                                                                                               >  2:724467/1‑150 (MQ=255)
       cTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGttt                                                                                                                                        <  1:893230/150‑1 (MQ=255)
           aaCGTTATGCCTGCTCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGt                                                                                                                                    <  2:518578/149‑1 (MQ=255)
             cGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCCAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGttt                                                                                                                                  <  1:1258298/150‑2 (MQ=38)
             cGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCGCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTg                                                                                                                                  <  2:314552/150‑1 (MQ=255)
              gTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTgg                                                                                                                                 >  1:58235/1‑150 (MQ=255)
                    ccTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAAc                                                                                                                           <  2:1129445/150‑1 (MQ=255)
                        cgcAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTg                                                                                                                       <  1:603276/150‑1 (MQ=255)
                         gcAAATGACCTATAAAGACTCGACAGTCAAGCTCAACATTCTCTAATACCGCACAGCTGGCATCAATTGCCGCGTCCAAAATTAAGCTCTAAATGCTGTCCNANCNAAAACGGCCCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTgg                                                                                                                      <  1:941607/150‑1 (MQ=255)
                                  cTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCCTCAATTGCCGCGGCCAAACTTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTgtc                                                                                                             >  2:533551/1‑148 (MQ=255)
                                    aTGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAGACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCt                                                                                                           <  2:520382/150‑1 (MQ=255)
                                    aTGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCt                                                                                                           >  1:1213987/1‑150 (MQ=255)
                                                       gCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACtt                                                                                        >  1:970999/1‑150 (MQ=255)
                                                        cTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTc                                                                                       >  2:1072563/1‑150 (MQ=255)
                                                           aaCATTCTCGAATACCGCGCAGCTGGCATCACCCGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGt                                                                                                                        <  1:1043241/114‑1 (MQ=255)
                                                           aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCTTTTAAGGCGCCGtt                                                                                                                                         <  2:1269561/97‑1 (MQ=39)
                                                           aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCTTTTAAGGCGCCGtt                                                                                                                                         >  1:1269561/1‑97 (MQ=39)
                                                           aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATc                                                                                    <  2:689002/150‑1 (MQ=255)
                                                           aaCATTCTCGAATACCGCGCAGCTGGCATCAACTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGt                                                                                                                        >  2:1043241/1‑114 (MQ=255)
                                                              aTTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCg                                                                                 >  1:771684/1‑150 (MQ=255)
                                                                          cgcgCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgtt                                                                     <  1:1072563/150‑1 (MQ=255)
                                                                              cAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGGGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTgaga                                                                 <  2:58235/150‑1 (MQ=255)
                                                                               aGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGAc                                                                >  2:1283875/1‑150 (MQ=255)
                                                                                      aTCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTg                                                                                                          >  1:356023/1‑101 (MQ=255)
                                                                                      aTCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTg                                                                                                          <  2:356023/101‑1 (MQ=255)
                                                                                      aTCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAAc                                                                                             >  2:852482/1‑114 (MQ=255)
                                                                                      aTCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGAc                                                         >  1:1166885/1‑150 (MQ=255)
                                                                                      aTCAATTGCCGCGGCAAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAAc                                                                                             <  1:852482/114‑1 (MQ=255)
                                                                                          aTTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTg                                                                                                               <  2:993533/92‑1 (MQ=255)
                                                                                          aTTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTg                                                                                                               >  1:993533/1‑92 (MQ=255)
                                                                                           ttGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTg                                                      >  2:687757/1‑148 (MQ=255)
                                                                                           ttGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTg                                                      <  1:687757/148‑1 (MQ=255)
                                                                                                   gCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATga                                            >  1:203997/1‑150 (MQ=255)
                                                                                                     cAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATCATAAGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                                          >  1:978741/1‑150 (MQ=255)
                                                                                                      aaaaTTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATtagag                                         >  2:1125948/1‑150 (MQ=255)
                                                                                                       aaaTTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTAAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgagaga                                        <  1:798223/150‑1 (MQ=255)
                                                                                                         aTTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGaca                                      <  2:1166885/150‑1 (MQ=255)
                                                                                                             aGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCAt                                  <  2:725281/150‑1 (MQ=255)
                                                                                                                tctGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGCTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCa                               >  2:771666/1‑150 (MQ=255)
                                                                                                                    aaTGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTc                           >  1:474013/1‑150 (MQ=255)
                                                                                                                            ccAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTg                   <  2:1213987/150‑1 (MQ=255)
                                                                                                                             cAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCAAATTCCATAccgag                  >  2:274026/1‑147 (MQ=255)
                                                                                                                              aaaCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGga                 >  2:900077/1‑150 (MQ=255)
                                                                                                                              aaaCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGga                 >  2:9117/1‑150 (MQ=255)
                                                                                                                              aaaCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACAACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGt                                                    <  2:507311/115‑1 (MQ=255)
                                                                                                                              aaaCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACAACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGt                                                    >  1:507311/1‑115 (MQ=255)
                                                                                                                                aCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATTAGAGACACCATCCACTTCCATACCTGgaga               <  1:1125948/150‑1 (MQ=255)
                                                                                                                                   aaaaCGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGcc            <  2:203997/150‑1 (MQ=255)
                                                                                                                                      aCGGCGCTTTTAAGGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACGGATGAACTGCTATACCTCAGAGACACCCTCCACTTCCATACCTGGAGAGCCTGc         >  2:599657/1‑150 (MQ=255)
                                                                                                                                           gcCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCa    >  2:1254709/1‑150 (MQ=255)
                                                                                                                                             cTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCGGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATa  >  1:614851/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATA  >  NC_002947/6129085‑6129375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑