| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,161,940 | T→C | 6.5% | F98L (TTT→CTT) | PP_5403 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,161,940 | 0 | T | C | 6.5% | 179.8 / 2.3 | 61 | F98L (TTT→CTT) | PP_5403 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (30/27); new base C (2/2); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
GGAGAGCGCTATGAGGTGCACCTATGTGAACCTTGCTTCTTCCAGACCATCGCTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTTCGTCGGTCGCCTTTGGAAGCGGTTTACGACGGG > NC_002947/6161796‑6162089 | ggAGAGCGCTATGAGGTGCACCTATGTGAACCTTGCTTCAACCAGTCTAGCGCTTACCACAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTc > 2:1008922/1‑150 (MQ=255) gagCGCTATGAGGTGCACCTATGTGAACCTTGCTTCTTCCAGACCATCGCTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGt < 2:1146728/150‑1 (MQ=255) cTATGAGGTGCACCTATGTGAACCTTGCTTCTTCCAGACCATCGCTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACCTTTTCCGTGATgg > 1:136979/1‑150 (MQ=255) tGAGGTGCACCTATGTGAACCTTGCTTCTTCCAGACCATCGCTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACttttt < 1:154112/138‑1 (MQ=255) tGAGGTGCACCTATGTGAACCTTGCTTCTTCCAGACCATCGCTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACttttt > 2:154112/1‑138 (MQ=255) cACCTATGTGAACCTTGCTTCTTCCAGACCATCGCTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACCTTTTCCGTGATGGTCACTGAttt < 2:136979/150‑1 (MQ=255) ttcttcCAGACCATCGCTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACAt > 2:352173/1‑150 (MQ=255) ccATCGCTTACCTCAATCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCgg < 2:917258/150‑1 (MQ=255) cTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGt < 1:575218/101‑1 (MQ=255) cTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGt > 2:575218/1‑101 (MQ=255) cTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTc > 1:1106277/1‑137 (MQ=255) cTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTc < 2:1106277/137‑1 (MQ=255) cTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAgggg > 2:37733/1‑150 (MQ=255) cTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAgggg > 2:1272050/1‑150 (MQ=255) tCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGaaa < 2:629438/114‑1 (MQ=255) tCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGaaa > 1:629438/1‑114 (MQ=255) aagcaagAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCGTCCAGGGATGACTTTTTCCGTGATGGTCACTGAGTTGAAAGCCAAGCTGGACATGCTTGATAGGCTTTTTAGGGGGTa > 1:390079/1‑150 (MQ=255) agCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACgg < 1:1003065/150‑1 (MQ=255) gcggcgCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATg < 1:1206140/89‑1 (MQ=255) gcggcgCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATg > 2:1206140/1‑89 (MQ=255) gcggcgCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACggg < 1:37733/150‑1 (MQ=255) cggcgCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATg > 2:554278/1‑88 (MQ=255) cggcgCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATg < 1:554278/88‑1 (MQ=255) gcgcATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTaa < 2:1017638/140‑1 (MQ=255) gcgcATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTaa > 1:1017638/1‑140 (MQ=255) tAACCAACTTGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGTCTTTTTAGGGGGTAAGGACGGGGACTGTGa > 2:165345/1‑150 (MQ=255) tAACCAACATGATTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGa > 1:1162181/1‑150 (MQ=255) aCCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGt > 1:958725/1‑150 (MQ=255) cAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGc > 1:453523/1‑94 (MQ=255) cAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGc < 2:453523/94‑1 (MQ=255) aaCATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGGCTTTTTCCGCGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAgggg < 2:888459/126‑1 (MQ=255) aaCATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCcg > 2:888261/1‑150 (MQ=255) aaCATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGCGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAgggg > 1:888459/1‑126 (MQ=255) ggTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTa > 2:368866/1‑110 (MQ=255) ggTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTa < 1:368866/110‑1 (MQ=255) gTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGaa > 1:1024652/1‑150 (MQ=255) tCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAgg < 2:105988/107‑1 (MQ=255) tCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAgg > 1:105988/1‑107 (MQ=255) tCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCt > 1:388110/1‑150 (MQ=255) ccACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGGCTGTGAGTCCGCGTATCGATGGAAGGCTa > 1:224426/1‑150 (MQ=255) acaGCACCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCCGGCAACCCTCCTTCGGCTCCTTACATCTTTAGGGCGTTGACTGTGAGTCCGCGTATCGATGGAAGGCTAGt < 1:350218/150‑1 (MQ=255) cagtCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTa < 1:9077/150‑1 (MQ=255) cagcCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGaa > 2:1205896/1‑139 (MQ=255) cagcCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGaa < 1:1205896/139‑1 (MQ=255) cccGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGAt > 2:510885/1‑132 (MQ=255) cccGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGAt < 1:510885/132‑1 (MQ=255) ccGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGa < 2:1142830/111‑1 (MQ=255) ccGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGa > 1:1142830/1‑111 (MQ=255) cGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTg > 1:1255392/1‑54 (MQ=255) cGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTg < 2:1255392/54‑1 (MQ=255) cGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGaaa < 1:722353/62‑1 (MQ=255) cGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGaaa > 2:722353/1‑62 (MQ=255) cGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGACAAGCCCGACATGCTAATCCGGCTTCATCAGCGGTAAGCCCCTGGACGGGGTGTTCGCGTATCGATGGAAGGCTATTGCTAGGTCt < 2:787791/150‑1 (MQ=255) aTGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTtcgtc < 2:795209/150‑1 (MQ=255) cTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTTCgtcggtc < 2:388110/150‑1 (MQ=255) ttCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTAc > 1:397819/1‑71 (MQ=255) ttCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTAc < 2:397819/71‑1 (MQ=255) cTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTTCGTCGGTCGCCTTTg < 2:1162181/150‑1 (MQ=255) tCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTTCGTCGGTCGCCTTTgg > 2:232917/1‑150 (MQ=255) aGGGATGACCTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGa < 2:839573/91‑1 (MQ=255) aGGGATGACCTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGa > 1:839573/1‑91 (MQ=255) aTGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTTCGTCGGTCGCCTTTGGAAGCGGTTTacg > 1:1047466/1‑150 (MQ=255) cTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTTCGTCGGTCGCCTTTGGAAGCGGTTTACGACgg < 2:1047466/150‑1 (MQ=255) tttttCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTtc < 1:278584/118‑1 (MQ=255) tttttCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTtc > 2:278584/1‑118 (MQ=255) tttttCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTTCGTCGGTCGCCTTTGGAAGCGGTTTACGACggg > 2:1202861/1‑150 (MQ=255) | GGAGAGCGCTATGAGGTGCACCTATGTGAACCTTGCTTCTTCCAGACCATCGCTTACCTCAAGCAAGAGCGGCGCATAACCAACATGTTTGAAGGTGATCCACAGCAGCCCGAGGATGACTTCGGCCTCGCTTCCAGGGATGACTTTTTCCGTGATGGTCACTGATTTGAAAGCCAAGCTGGACATGCTACTTCGGCTTTTTAGGGGGTAAGGACGGGGACTGTGAGTCCGCGTATCGATGGAAGGCTAGTGCTAGGTCTTCGTCGGTCGCCTTTGGAAGCGGTTTACGACGGG > NC_002947/6161796‑6162089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |