Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	417,375	C→T	11.6%	P361L (CCG→CTG)	waaG →	lipopolysaccharide glucosyltransferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	417,375	0	C	T	11.6%	148.4 / 10.4	62	P361L (CCG→CTG)	waaG	lipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base C (28/25);  new base T (4/3);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

GGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGC  >  NC_002947/417226‑417520                                                                                                                                                      |                                                                                                                                                  ggCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGcc                                                                                                                                                   <  1:536155/150‑1 (MQ=255)  gCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCg                                                                                                                                                  <  2:616459/150‑1 (MQ=255)     gACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCgcag                                                                                                                                               >  1:250759/1‑150 (MQ=255)        aGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAt                                                                                                                                            <  2:1012954/150‑1 (MQ=255)                 gTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGAc                                                                                                                                   >  1:703077/1‑150 (MQ=255)                            gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg                                                                                                                        <  1:1002362/150‑1 (MQ=255)                            gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg                                                                                                                        >  1:537456/1‑150 (MQ=255)                            gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg                                                                                                                        <  1:317212/150‑1 (MQ=255)                                ttCGAACAAGCGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGCTAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCaa                                                                                                                    <  2:194753/150‑1 (MQ=255)                                ttCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctgta                                                                                                                                      <  1:1171805/132‑3 (MQ=255)                                ttCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctgta                                                                                                                                      >  2:1171805/1‑130 (MQ=255)                                     aCAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAAc                                                                                                               <  1:1161726/150‑1 (MQ=255)                                       aggggcagcaCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCg                                                                                                             >  2:690356/5‑150 (MQ=255)                                          agcagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCAt                                                                                                          <  1:420591/150‑1 (MQ=255)                                          agcagcTCAACGGCTATCTGCAGCGCATGCTCAAAAACAAGCACGCGCGTGAACGGTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCAt                                                                                                          >  2:1009034/1‑150 (MQ=255)                                                tCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCt                                                                                                    >  2:291671/1‑150 (MQ=255)                                                 cAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTg                                                                                                   >  2:950751/1‑150 (MQ=255)                                                 aaaCGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTg                                                                                                   <  2:442003/149‑1 (MQ=255)                                                     ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctgta                                                                                                                                      >  2:493973/1‑109 (MQ=255)                                                     ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctgta                                                                                                                                      <  1:493973/111‑3 (MQ=255)                                                        tATCTGCAGCGCGTGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTgg                                                                                            <  2:167064/150‑1 (MQ=255)                                                          tCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGcc                                                                                          <  2:1265004/150‑1 (MQ=255)                                                                  gcATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGACGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCtt                                                                                  <  2:766171/150‑1 (MQ=255)                                                                    aTGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa                                                                                >  1:887983/1‑150 (MQ=255)                                                                        tCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGAc                                                                                                                                   >  1:365860/1‑95 (MQ=255)                                                                        tCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGAc                                                                                                                                   <  2:365860/95‑1 (MQ=255)                                                                         cGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGc                                                                           <  2:703077/150‑1 (MQ=255)                                                                          gaagaaCCGCAGGCGCGTGCCAGCTGGGCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCGGTACAGCATGCGGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGcc                                                                          >  2:292079/1‑150 (MQ=255)                                                                          gAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGcc                                                                          >  1:1243755/1‑150 (MQ=255)                                                                           aaGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCt                                                                         <  2:537456/150‑1 (MQ=255)                                                                            aGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCtg                                                                        <  1:1214842/150‑1 (MQ=255)                                                                                ccGCAGGCGCGTGCCAGCAGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCACACCATGCTGCCGACGTGATCCTGGGACAAAAAACCGCATGAAGCTGATATTGGCAGAACCCTTGAAACGCCTGTggg                                                                    >  2:460443/1‑150 (MQ=255)                                                                                      gcgcGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCaaa                                                                              <  1:922611/134‑1 (MQ=255)                                                                                      gcgcGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCaaa                                                                              >  2:922611/1‑134 (MQ=255)                                                                                           tGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGAt                                                         <  1:690356/150‑1 (MQ=255)                                                                                             ccAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGATCCCTTCAAACGCCTGTGGGCCGGGCGTGATGc                                                       <  1:159108/150‑1 (MQ=255)                                                                                                    gTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCgg                                                                <  2:62746/134‑1 (MQ=255)                                                                                                    gTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCgg                                                                >  1:62746/1‑134 (MQ=255)                                                                                                       gcgcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGcc                                             >  1:37600/1‑150 (MQ=255)                                                                                                               tcttGGCATTTGCTGATAGCGTCGATCTGTACAGCATGCAGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGgc                                     <  2:530410/147‑1 (MQ=255)                                                                                                                ccTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTggg                                                                    <  2:454186/118‑1 (MQ=255)                                                                                                                ccTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTggg                                                                    >  1:454186/1‑118 (MQ=255)                                                                                                                       tttGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAg                             >  2:953332/1‑150 (MQ=255)                                                                                                                            tGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGATGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg                        >  1:326558/1‑150 (MQ=255)                                                                                                                                   gcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCGTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACTCCTGTTGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATc                 >  2:182250/1‑150 (MQ=255)                                                                                                                                   gcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATc                 >  2:610931/1‑150 (MQ=255)                                                                                                                                     cGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATcgc               >  2:487035/1‑150 (MQ=255)                                                                                                                                     cGATCGGTACAGCAGGCCGCAGCATGCTGCCGACGTGATCCGGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTTGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATcgc               <  1:1184359/150‑1 (MQ=255)                                                                                                                                       aTCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCGTGGGGGCCGGGCGTGGTGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATGGCGa             >  1:271290/1‑150 (MQ=255)                                                                                                                                       aTCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGa             >  1:270536/1‑150 (MQ=255)                                                                                                                                       aTCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGa             >  2:1188374/1‑150 (MQ=255)                                                                                                                                        tCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAg            <  1:950751/150‑1 (MQ=255)                                                                                                                                          tGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAgg                                      >  1:120888/1‑122 (MQ=255)                                                                                                                                          tGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAgg                                      <  2:120888/122‑1 (MQ=255)                                                                                                                                           gTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCTGAATCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTg         >  1:291418/1‑150 (MQ=255)                                                                                                                                            tACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCt          <  2:266613/148‑1 (MQ=255)                                                                                                                                            tACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCt          >  1:266613/1‑148 (MQ=255)                                                                                                                                            ggcAGCATGCTGCGGCATGCTGTCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCAAATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTgg        >  2:175015/3‑150 (MQ=255)                                                                                                                                             gcAGCATGCTGCGGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg                        <  2:696241/132‑1 (MQ=255)                                                                                                                                             gcAGCATGCTGCGGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg                        >  1:696241/2‑133 (MQ=255)                                                                                                                                               aGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAg     >  1:196154/1‑150 (MQ=255)                                                                                                                                                 cATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAggg   <  2:982857/150‑1 (MQ=255)                                                                                                                                                  aTGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAgggg  >  1:1095772/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                  GGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGC  >  NC_002947/417226‑417520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑